Feather pulp: a novel substrate useful for proton nuclear magnetic resonance spectroscopy metabolomics and biomarker discovery

Brown, Catherine L. J.; Montina, Tony; Inglis, G. Douglas

doi:10.1016/j.psj.2022.101866

Cited by 3 publications

(2 citation statements)

References 104 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although there are many potential means of assessing stress, metabolomics has shown promise as a highly sensitive method of determining a stress condition based on relative concentrations of metabolites in biological samples [ 24 ]. In fact, recent nuclear magnetic resonance (NMR) spectroscopy-based metabolomics studies have shown that the metabolomes of eggs, breast muscle, liver, kidney, feces, and feathers can be used in a variety of ways to better understand domestic poultry physiology, including stress [ 6 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 ]. However, the use of metabolomics to identify biomarkers of health in poultry remains in its infancy.…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of the Temporal Impacts of Corticosterone and Simulated Production Stressors on the Metabolome of Broiler Chickens

et al. 2023

Self Cite

View full text Add to dashboard Cite

The impact of physiological stress on the metabolome of breast muscle, liver, kidney, and hippocampus was investigated in Ross 308 broiler chicks. Simulated on-farm stressors were compared to a corticosterone model of physiological stress. The three different stressors investigated were: (i) corticosterone at a dose of 15 mg/kg of feed; (ii) heat treatment of 36 °C and 40% RH for 8 h per day; and (iii) isolation for 1 h per day. Liver, kidney, breast muscle, and hippocampus samples were taken after 2, 4, 6, and 8 days of stress treatment, and subjected to untargeted 1H-nuclear magnetic resonance (NMR) spectroscopy-based metabolomic analysis to provide insights on how stress can modulate metabolite profiles and biomarker discovery. Many of the metabolites that were significantly altered in tissues were amino acids, with glycine and alanine showing promise as candidate biomarkers of stress. Corticosterone was shown to significantly alter alanine, aspartate, and glutamate metabolism in the liver, breast, and hippocampus, while isolation altered the same pathways, but only in the kidneys and hippocampus. Isolation also significantly altered the glycine, serine, and threonine metabolism pathway in the liver and breast, while the same pathway was significantly altered by heat in the liver, kidneys, and hippocampus. The study’s findings support corticosterone as a model of stress. Moreover, a number of potential metabolite biomarkers were identified in chicken tissues, which may allow producers to effectively monitor stress and to objectively develop and evaluate on-farm mitigations, including practices that reduce stress and enhance bird health.

show abstract

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of the Temporal Impacts of Corticosterone and Simulated Production Stressors on the Metabolome of Broiler Chickens

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…Third, metabolomics data are easy to analyse. Fourth, sample collection and pretreatment are convenient and can be performed with general technology [5,6]. Because of these advantages, metabolomics technology has been applied in the field of assisted reproductive technology (ART).…”

Section: Introductionmentioning

confidence: 99%

Metabolomics and its applications in assisted reproductive technology

Gao

Yan

2023

IET Nanobiotechnology

View full text Add to dashboard Cite

Metabolomics, an emerging omics technology developed in the post‐gene age, is an important part of systems biology. It interprets the pathophysiological state of the subject by quantitatively describing the dynamic changes of metabolites through analytical methods, mainly mass spectrometry (MS) and nuclear magnetic resonance (NMR). Assisted reproductive technology (ART) is a method used to manipulate sperm, oocytes, and embryos to achieve conception. Recently, several studies have reported that metabolomics methods can be used to measure metabolites in ART samples; these metabolites can be used to evaluate the quality of gametes and embryos. This article reviews the progress of research on metabolomics and the application of this technology in the field of ART, thus providing a reference for research and development directions in the future.

show abstract

Indigenous Knowledge and Phytochemistry: Deciphering the Healing Power of Herbal Medicine

Jacob,

Izah,

Nelson

et al. 2023

Reference Series in Phytochemistry

View full text Add to dashboard Cite

Feather pulp: a novel substrate useful for proton nuclear magnetic resonance spectroscopy metabolomics and biomarker discovery

Cited by 3 publications

References 104 publications

Comparative Analysis of the Temporal Impacts of Corticosterone and Simulated Production Stressors on the Metabolome of Broiler Chickens

Comparative Analysis of the Temporal Impacts of Corticosterone and Simulated Production Stressors on the Metabolome of Broiler Chickens

Metabolomics and its applications in assisted reproductive technology

Indigenous Knowledge and Phytochemistry: Deciphering the Healing Power of Herbal Medicine

Contact Info

Product

Resources

About