Feather HSP70: a novel non-invasive molecular marker for monitoring stress induced by heat exposure in broilers

Greene, Elizabeth S.; Rajaei-Sharifabadi, Hossein; Dridi, Sami

doi:10.3382/ps/pez120

Cited by 20 publications

(9 citation statements)

References 27 publications

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“…Large, abrupt, and widespread extreme heat waves have occurred repeatedly in the past (7), and predicted to increase for the next century (8). In modern broiler chickens, which supports the livelihoods and food security of billions of people (5), the strong negative effect of heat stress on growth, feed efficiency, meat yield and mortality is well documented (10)(11)(12)(13)(14)(15)(16)(17)(18), and such effects will take a heavy toll during the next decades as the distribution of heat anomalies continues to increase (9). Aforesaid effects will inflict heavy economic losses to the poultry industry (34).…”

Section: Discussionmentioning

confidence: 99%

“…Due to their high metabolic activity and lack of sweat glands, modern broilers are particularly sensitive to heat stress. The strong adverse effects of heat stress on growth, feed intake, feed efficiency, meat yield and quality, welfare, and mortality are well documented in broilers (10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20). Emerging evidence indicates that heat stress alters also the gut morphometry (lower crypt depth, mucous area, and villus height) which in turn affect nutrient absorption (21).…”

Section: Introductionmentioning

confidence: 99%

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Intestinal Barrier Integrity in Heat-Stressed Modern Broilers and Their Ancestor Wild Jungle Fowl

et al. 2020

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High environmental temperature has strong adverse effects on poultry production, welfare, and sustainability and, thereby, constitutes one of the most challenging stressors. Although colossal information has been published on the effects of heat stress on poultry productivity and gut health, the fundamemntal mechanisms associated with heat stress responses and intestinal barrier function are still not well defined. The aim of the present study was, therefore, to determine the effects of acute (2 h) heat stress on growth performance, gut integrity, and intestinal expression of heat shock and tight junction proteins in slow-(broilers of the 1950's, ACRB), moderate-(broilers of 1990's, 95RAN), rapid-(modern broilers, MRB) growing birds, and their ancestor wild jungle fowl (JF). Heat stress exposure significantly increased the core body temperature of 95RAN and MRB chickens by ∼0.5-1 • C, but not that of JF and ACRB compared to their counterparts maintained at thermoneutral conditions. Heat stress also depressed feed intake and increased serum fluorescein isothiocyanate-dextran (FITC-D) levels (P < 0.05) in modern broilers (95RAN and MRB) but not in JF and ACRB, indicating potential leaky gut syndrome. Molecular analyses showed that heat stress exposure significantly up regulated the duodenal expression of occludin (OCLN) and lipocalin (LCN2) in ACRB, zonula occludens (ZO-2), villin1 (VIL1), and calprotectin (CALPR) in 95 RAN, and only CALPR in MRB compared to their TN counterparts. In the jejunum however, heat stress down regulated the expression of PALS1-associated tight junction protein (PATJ) in ACRB, 95RAN, and MRB, and that of cadherin1 (CDH1) in MRB. In the ileum, heat stress significantly down regulated the expression of OCLN in 95 RAN, ZO-1 in MRB, gap junction protein alpha1 (GJA1) in JF, and VIL1 in ACRB compared to their TN counterparts. In summary, this is the first report, to our knowledge, showing that tight junction protein expression is environmental-, genotype-, and intestinal segment-dependent and identifying molecular signatures, such as CDH1, CALPR, and ZO-1, potentially involved in leaky gut syndrome-induced by heat stress in MRB.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Intestinal Barrier Integrity in Heat-Stressed Modern Broilers and Their Ancestor Wild Jungle Fowl

et al. 2020

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“…It has been reported that heat shock protein protects birds from high temperatures by preventing unwanted protein aggregation and channelizing their degradation [36]. The expression of mRNA HSP70 was measured in the gut [14,37], liver [38], hypothalamus [27,39], and blood and feather [40]. Here, we focused on detecting HSP70 in ileum because it plays a vital role in digestion and absorption, as well as immunity.…”

Section: Discussionmentioning

confidence: 99%

Threonine Requirements in Dietary Low Crude Protein for Laying Hens under High-Temperature Environmental Climate

Azzam

Alhotan

Al-Abdullatif

et al. 2019

Animals

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Simple SummaryThe threonine (Thr) requirement of laying hens in a high-temperature climate is scarcely referred in the review of literature. Therefore, our aim was to estimate the dietary Thr requirement in low CP diets in a high-temperature environmental climate. Based on our findings, the optimal dietary Thr requirements to optimize egg production, serum uric acid, and serum CuZn-SOD were 0.58%, 0.59%, and 0.56%, respectively, by regression analysis.AbstractLohmann Brown hens (n = 420), at 28 weeks of age, were divided into five dietary treatments, and each treatment included six replicates of 14 laying hens. Dietary crude protein (14%) was presented as the control diet. Dietary L-Thr was added to the control diet for 12 weeks. Dietary Thr levels are 0.43%, 0.49%, 0.57%, 0.66%, and 0.74%, based on digestible base. From 28 to 40 weeks, hen-day egg production presented a quadratic trend to supplementing dietary Thr (R2 = 0.96, p = 0.02), and reached a maximum level at 0.58%. Serum uric acid demonstrated a quadratic trend (R2 = 0.62, p = 0.02) at 0.59%. Both serum total cholesterol and 3-hydroxy-3-methylglutaryl (HMG-CoA) reductase showed lower levels (p < 0.05) at 0.66% Thr. Serum CuZn-SOD elevated (p < 0.05) at 0.49%, 0.57%, and 0.66% Thr, as compared to the control group, and showed a quadratic trend (R2 = 0.87, p = 0.003) at 0.56%. Supplemental L-Thr decreased (p < 0.05) the expression of ileal HSP70 at 0.66% Thr. In summary, the optimal dietary Thr requirements to optimize egg production, serum uric acid, and serum CuZn-SOD were 0.58%, 0.59%, and 0.56%, respectively, by regression analysis.

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“…Under heat and other stress conditions, HSF combines with the heat shock promoter element (HSE) to activate the transcriptional synthesis of HSP70 and increase the expression of HSP70. The level of HSP70 is positively correlated with temperature under high-temperature stress [10][11][12][13]. HSP70 is considered to be an important protective molecule that measures the functional state and anti-stress ability of cells under stress [14].…”

Section: Introductionmentioning

confidence: 99%

Effect of Glutamine on Antioxidant Capacity and Lipid Peroxidation in the Breast Muscle of Heat-stressed Broilers via Antioxidant Genes and HSP70 Pathway

Chen

Dai

et al. 2020

Animals

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This study investigated whether Glutamine (Gln) could be used as an additive to improve antioxidant capacity in the breast muscle of heat-stressed broilers. Two hundred and forty 22-day-old Arbor Acres broilers in the G1, G2, G3, and G4 groups (n = 60 each) were housed in a cyclic hot environment and fed the basal diet with 0%, 0.5%, 1.0%, and 1.5% Gln, respectively. Compared with the G1 group, dietary 1.5% Gln increased (p < 0.05) pH and b* values, but decreased (p < 0.05) L* cooking loss, drip loss, and water loss rate in breast meat of heat-stressed broilers. Malondialdehyde levels in the breast muscle were lower (p < 0.05) in 1.0% and 1.5% Gln groups than that of the heat-stress group. Compared with the G1 group, dietary 1.5% Gln increased (p < 0.05) catalase (CAT), glutathione, glutathione peroxidase (GSH-Px,) and total antioxidant capacity in the breast muscle of heat-stressed broilers. Furthermore, the CAT, GSH-Px, HSP70 mRNA expression levels, and HSP70 protein expression levels were increased (p < 0.05) in the G3 and G4 groups compared with the G1 group. In sum, Gln alleviated antioxidant capacity and lipid peroxidation in the breast muscle of heat-stressed broilers through antioxidant genes and HSP70 pathways.

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Feather HSP70: a novel non-invasive molecular marker for monitoring stress induced by heat exposure in broilers

Cited by 20 publications

References 27 publications

Intestinal Barrier Integrity in Heat-Stressed Modern Broilers and Their Ancestor Wild Jungle Fowl

Intestinal Barrier Integrity in Heat-Stressed Modern Broilers and Their Ancestor Wild Jungle Fowl

Threonine Requirements in Dietary Low Crude Protein for Laying Hens under High-Temperature Environmental Climate

Effect of Glutamine on Antioxidant Capacity and Lipid Peroxidation in the Breast Muscle of Heat-stressed Broilers via Antioxidant Genes and HSP70 Pathway

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