Feasible Options to Control Colonization of Enteric Pathogens With Designed Synbiotics

Peng, Mengfei; Patel, Parshva; Vinod, Nagarajan; Bernhardt, Cassandra; Carrion, Michael; Biswas, Debabrata

doi:10.1016/b978-0-12-814468-8.00011-9

Cited by 12 publications

(6 citation statements)

References 103 publications

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“…When comparing between prebiotics and synbiotics, Kridtayopas et al (2019) suggested that using synbiotics seems to have more benefits than using prebiotics on HSD broilers. In this case, the benefits of prebiotics, the function of probiotics, and the combinational effectiveness or synergistic effects of both are typically credited with synbiotics' more promising effects (Peng et al, 2019). The efficiency of prebiotics in ameliorating the negative effects of overcrowding stress on broilers appears to be influenced by the populations of birds per square meter, housing conditions and management used during rearing.…”

Section: Application Of Prebiotics and Synbiotics In Broilers Raised At A High Stocking Densitymentioning

confidence: 99%

Dietary strategies to alleviate high-stocking-density-induced stress in broiler chickens – a comprehensive review

Sugiharto

2022

Arch. Anim. Breed.

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Abstract. Stocking broilers at a high density has been a strategy to optimize the area of the cage and hence increase the efficiency of broiler production. If the environmental (microclimate) conditions and rearing management are not properly managed, stocking broilers at a high density may, however, result in stressful conditions that are harmful for the production, health and welfare of broilers. To ameliorate these unfavorable effects of overcrowding stress, dietary interventions have been conducted. Probiotics, prebiotics, synbiotics, plant-derived products, vitamins, propolis, amino acids, fatty acids, etc. have been supplemented in diets to deal with the harmful impact of stress induced by a high stocking density of broilers. This review covers the detrimental effects of overcrowding-induced stress on broiler development and attempts to ameliorate those negative effects by dietary interventions.

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Section: Application Of Prebiotics and Synbiotics In Broilers Raised At A High Stocking Densitymentioning

confidence: 99%

Dietary strategies to alleviate high-stocking-density-induced stress in broiler chickens – a comprehensive review

Sugiharto

2022

Arch. Anim. Breed.

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“…17,18 In these situations, procommensal strategies by application of probiotics, prebiotics, and synbiotics can be considered as priority in prevention and treatment of such foodborne bacterial pathogen-induced enteric illness. 13,19,20 With a promising scheme, it allows an establishment or recovery of the healthy enteric microbial ecosystem by introducing native, exogenous, or genetically engineered beneficial probiotics without inducing deleterious effects (like antibiotics) on human commensal gut bacteria. 16,21 Recently, we constructed and reported the role of a multi-functional Lactobacillus casei probiotic strain overexpressing myosin cross-reactive antigen gene (mcra), named as LC +mcra.…”

Section: Introductionmentioning

confidence: 99%

Prevention of enteric bacterial infections and modulation of gut microbiota with conjugated linoleic acids producing Lactobacillus in mice

et al. 2019

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Probiotics are recognized for outcompeting pathogenic bacteria by competitive receptormediated colonization and secretion of functional metabolites which are antimicrobial against certain microbes as well as improving host's gut health and immunity. Recently, we have constructed a bioactive Lactobacillus casei (LC) strain, LC +mcra , by inserting mcra (myosin crossreactive antigen) gene, which stimulates the conversion of conjugated linoleic acids. In this study, we evaluated the modulation of gut microbiome and protective roles of LC +mcra against pathogenic Salmonella enterica serovar Typhimurium (ST) and enterohemorrhagic E. coli (EHEC) infections in BALB/cJ mice. We observed that LC +mcra colonized efficiently in mice gut intestine and competitively reduced the infection with ST and EHEC in various locations of small and large intestine, specifically cecum, jejunum, and ileum (p < 0.05). Positive modulation of the cecal microbiota, for example, higher relative abundances of Firmicutes, lower relative abundances of Proteobacteria, and increased bacterial species diversity/richness, was detected in ST-challenged mice pretreated with LC +mcra based on 16S metagenomic sequencing. Cytokine gene expression analysis indicated that mice pretreated with LC +mcra associated with attenuated bacterial pathogen-induced gut inflammation. Furthermore, mice fed daily with LC +mcra for one week could protect themselves from the impairments caused by enteric infections with ST or EHEC. These impairments include weight loss, negative hematological changes, intestinal histological alterations, and potential death. This in vivo study suggests that daily consumption of novel conjugated linoleic acids over-producing probiotic effectively improves intestinal microbiota composition and prevents/combats foodborne enteric bacterial infections with pathogenic Salmonella and diarrheagenic E. coli.

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“…Probiotics, as bio-agents, can be considered the priority in prevention and control of foodborne bacterial pathogen-induced enteric illness ( Amalaradjou and Bhunia, 2012 ; Hayes and Vargas, 2016 ; Peng and Biswas, 2017 ; Peng et al, 2018a ). Through colonizing the host’s gastrointestinal (GI) tract, these beneficial bacteria ferment or metabolize undigested dietary components; after reaching the small and large intestine, the probiotics generate/release a tremendous treasury of secondary metabolites (byproducts), most of which are associated with multiple health benefits ( Flint et al, 2012 ; Marcobal et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Linoleic Acids Overproducing Lactobacillus casei Limits Growth, Survival, and Virulence of Salmonella Typhimurium and Enterohaemorrhagic Escherichia coli

et al. 2018

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Probiotics, particularly lactic acid bacteria, are biologic agents which limit the growth, virulence, and survival/colonization of various enteric bacterial pathogens and serve as potential alternatives to antibiotics. Mechanisms that contribute to this antimicrobial effect include producing bioactive metabolites/acids, increasing nutrient and receptor-mediated competition, and modulating gut microbiome ecology. However, these functions of common probiotic strains are limited due to the finite quantity of metabolites they produce and their total number in the gut ecosystem. Conjugated linoleic acids (CLAs), critical metabolites of Lactobacillus, have multiple beneficial effects on human health including anti-carcinogenesis, anti-inflammation, anti-oxidation, and anti-pathogenicity. In this study, we aim to overexpress the myosin cross-reactive antigen gene (mcra) in Lactobacillus casei (LC) to enhance the production of CLA and investigate its effectiveness against enteric bacterial pathogens, specifically Salmonella enterica serovar Typhimurium (ST) and enterohaemorrhagic Escherichia coli (EHEC). By inserting mcra in L. casei, we generated LC-CLA and found the total linoleic acid production by an individual bacterial cell was raised by 21-fold. The adherence ability of LC-CLA on human epithelial cells increased significantly and LC-CLA competitively excluded both ST and EHEC in a mixed-culture condition. Furthermore, LC-CLA significantly altered the physicochemical properties, biofilm formation abilities, interactions with host cells of both ST and EHEC, and triggered anti-inflammatory activities of host cells. These findings offer insights on applying a genetically engineered probiotic to control gut intestinal infections caused by ST and EHEC and prevent foodborne enteric illness in human.

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Feasible Options to Control Colonization of Enteric Pathogens With Designed Synbiotics

Cited by 12 publications

References 103 publications

Dietary strategies to alleviate high-stocking-density-induced stress in broiler chickens – a comprehensive review

Dietary strategies to alleviate high-stocking-density-induced stress in broiler chickens – a comprehensive review

Prevention of enteric bacterial infections and modulation of gut microbiota with conjugated linoleic acids producing Lactobacillus in mice

Linoleic Acids Overproducing Lactobacillus casei Limits Growth, Survival, and Virulence of Salmonella Typhimurium and Enterohaemorrhagic Escherichia coli

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