Feasibility study for intraepidermal delivery of proteins using a solid microneedle array

Witting, Madeleine; Obst, Katja; Pietzsch, Markus; Frieß, Wolfgang; Hedtrich, Sarah

doi:10.1016/j.ijpharm.2015.03.046

Cited by 36 publications

(11 citation statements)

References 32 publications

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“…The dip-coating technique with a mask has been used by other investigators (Andrianov et al, 2009). In a variation of the masked dip-coating method, a single orifice, which is often a small-diameter pipette tip (Chong et al, 2013) or a hypodermic needle (Witting et al, 2015) has also been used to dip coat the microneedles. Conceptually, the single orifice of the pipette or the hypodermic needle can be considered equivalent to a single masked hole that allows selective contact of the microneedle with the coating solution.…”

Section: Coating Methods That Selectively Coat the Microneedle Shaftsmentioning

confidence: 99%

Microneedle Coating Methods: A Review with a Perspective

Ingrole¹,

Gill²

2019

J Pharmacol Exp Ther

121

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A coated microneedle array comprises sharp micrometer-sized needle shafts attached to a base substrate and coated with a drug on their surfaces. Coated microneedles are under investigation for drug delivery into the skin and other tissues, and a broad assortment of active materials, including small molecules, peptides, proteins, deoxyribonucleic acids, and viruses, have been coated onto microneedles. To coat the microneedles, different methods have been developed. Some coating methods achieve selective coating of just the microneedle shafts, whereas other methods coat not only microneedle shafts but also the array base substrate. Selective coating of just the microneedle shafts is more desirable since it provides control over drug dosage, prevents drug waste, and offers high delivery efficiency. Different excipients are added to the coating liquid to modulate its viscosity and surface tension in order to achieve uniform coatings on microneedles. Coated microneedles have been used in a broad range of biomedical applications. To highlight these different applications, a table summarizing the different active materials and the amounts coated on microneedles is provided. We also discuss factors that should be considered when deciding suitability of coated microneedles for new-drug delivery applications. In recent years, many coated microneedles have been investigated in human clinical trials, and there is now a strong effort to bring the first coated microneedle-based product to market.

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Section: Coating Methods That Selectively Coat the Microneedle Shaftsmentioning

confidence: 99%

Microneedle Coating Methods: A Review with a Perspective

Ingrole¹,

Gill²

2019

J Pharmacol Exp Ther

121

View full text Add to dashboard Cite

show abstract

“…Up to a length of 500 μm, application of MNs is painless as they are too short to activate nerves in the dermis. MNs have been successfully used for intradermal delivery of DNA [ 63 ], siRNA [ 64 ], proteins [ 65 , 66 ], and peptides [ 67 ]. Especially hollow MNs seem promising for the topical delivery of nucleic acids [ 61 ].…”

Section: Delivery Of Gene Therapies To the Skinmentioning

confidence: 99%

Gene Delivery to the Skin – How Far Have We Come?

Ain

Campos

Huynh

et al. 2021

Trends in Biotechnology

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Gene therapies are powerful tools to prevent, treat, and cure human diseases. The application of gene therapies for skin diseases received little attention so far, despite the easy accessibility of skin and the urgent medical need. A major obstacle is the unique barrier properties of human skin, which significantly limits the absorption of biomacromolecules, and thus hampers the efficient delivery of nucleic acid payloads. In this review, we discuss current approaches, successes, and failures of cutaneous gene therapy and provide guidance toward the development of next-generation concepts. We specifically allude to the delivery strategies as the major obstacle that prevents the full potential of gene therapies – not only for skin disorders but also for almost any other human disease.

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“…Witting et al . conducted a feasibility study of the intraepidermal delivery of a model protein, asparaginase B (AsnB) [237]. Insertion experiments in pig's skin were performed where CMN coating dissolved in few minutes and the protein diffused homogenously, indicating coating was stable enough to withstand the insertion process.…”

Section: Microneedle-based Drug Deliverymentioning

confidence: 99%

Recent advances of controlled drug delivery using microfluidic platforms

Sanjay

Zhou

Dou

et al. 2018

Advanced Drug Delivery Reviews

278

163

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Conventional systematically-administered drugs distribute evenly throughout the body, get degraded and excreted rapidly while crossing many biological barriers, leaving minimum amounts of the drugs at pathological sites. Controlled drug delivery aims to deliver drugs to the target sites at desired rates and time, thus enhancing the drug efficacy, pharmacokinetics, and bioavailability while maintaining minimal side effects. Due to a number of unique advantages of the recent microfluidic lab-on-a-chip technology, microfluidic lab-on-a-chip has provided unprecedented opportunities for controlled drug delivery. Drugs can be efficiently delivered to the target sites at desired rates in a well-controlled manner by microfluidic platforms via integration, implantation, localization, automation, and precise control of various microdevice parameters. These features accordingly make reproducible, on-demand, and tunable drug delivery become feasible. On-demand self-tuning dynamic drug delivery systems have shown great potential for personalized drug delivery. This review presents an overview of recent advances in controlled drug delivery using microfluidic platforms. The review first briefly introduces microfabrication techniques of microfluidic platforms, followed by detailed descriptions of numerous microfluidic drug delivery systems that have significantly advanced the field of controlled drug delivery. Those microfluidic systems can be separated into four major categories, namely drug carrier-free micro-reservoir-based drug delivery systems, highly integrated carrier-free microfluidic lab-on-a-chip systems, drug carrier-integrated microfluidic systems, and microneedles. Microneedles can be further categorized into five different types, i.e. solid, porous, hollow, coated, and biodegradable microneedles, for controlled transdermal drug delivery. At the end, we discuss current limitations and future prospects of microfluidic platforms for controlled drug delivery.

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Feasibility study for intraepidermal delivery of proteins using a solid microneedle array

Cited by 36 publications

References 32 publications

Microneedle Coating Methods: A Review with a Perspective

Microneedle Coating Methods: A Review with a Perspective

Gene Delivery to the Skin – How Far Have We Come?

Recent advances of controlled drug delivery using microfluidic platforms

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