Feasibility study for cell electroporation detection and separation by means of dielectrophoresis

Oblak, Jakob; Križaj, Dejan; Amon, S.; Maček-Lebar, Alenka; Miklavčič, Damijan

doi:10.1016/j.bioelechem.2007.04.001

Cited by 46 publications

(33 citation statements)

References 30 publications

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“…[2][3][4] For most biological cells consisting of cytoplasm and plasma membrane, spherical single-shell model has been widely used by researchers to represent blood cells 5,6 and cancer cells. [7][8][9][10][11] In some cases, such as plants and most micro-organisms where the cell is surrounded by a cell wall, two-shell dielectric models were used. 3 Some bacteria, like Escherichia coli, are rod-shape; hence, prolate spheroidal core-shell models 12,13 were developed and applied.…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoretic capture voltage spectrum for measurement of dielectric properties and separation of cancer cells

Wu¹,

Yung²,

Lim³

2012

Biomicrofluidics

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In this paper, a new dielectrophoresis (DEP) method based on capture voltage spectrum is proposed for measuring dielectric properties of biological cells. The capture voltage spectrum can be obtained from the balance of dielectrophoretic force and Stokes drag force acting on the cell in a microfluidic device with fluid flow and strip electrodes. The method was demonstrated with the measurement of dielectric properties of human colon cancer cells . From the capture voltage spectrum, the real part of Clausius-Mossotti factor of HT-29 cells for different frequencies of applied electric field was obtained. The dielectric properties of cell interior and plasma membrane were then estimated by using single-shell dielectric model. The cell interior permittivity and conductivity were found to be insensitive to changes in the conductivity of the medium in which the cells are suspended, but the measured permittivity and conductivity of cell membrane were found to increase with the increase of medium conductivity. In addition, the measurement of capture voltage spectrum was found to be useful in providing the optimum operating conditions for separating HT-29 cells from other cells (such as red blood cells) using dielectrophoresis. V C 2012 American Institute of Physics. [http://dx

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Section: Introductionmentioning

confidence: 99%

Dielectrophoretic capture voltage spectrum for measurement of dielectric properties and separation of cancer cells

Wu¹,

Yung²,

Lim³

2012

Biomicrofluidics

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“…interior electrodes) embedded inside the microfluidic network is the common practice for the alternating current dielectrophoresis (AC-DEP) applications. Those electrodes are fabricated by using relatively more complex and expensive manufacturing techniques [18,23] such as vapor deposition [15], e-beam evaporation [14] and sputter coating [19,20,24]. Moreover, AC-DEP is usually used to do discrete separation (i.e.…”

Section: Introductionmentioning

confidence: 99%

Continuous particle separation by size via AC‐dielectrophoresis using a lab‐on‐a‐chip device with 3‐D electrodes

Çetin

Kang

et al. 2009

Electrophoresis

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In this paper, we present a novel, simple lab-on-a-chip device for continuous separation of particles by size. The device is composed of a straight rectangular microchannel connecting two inlet reservoirs and two exit reservoirs. Two asymmetric, 3-D electrodes are embedded along the channel wall to generate a non-uniform electrical field for dielectrophoresis. Particles with different sizes are collected at the different exit reservoirs. Main flow is induced by pressure difference between the inlet and the exit reservoirs. The device is used successfully for the separation of the 5 and 10 mum latex particles and for the separation of yeast cells and white blood cells. A numerical simulation based on Lagrangian tracking method is used to simulate the particle motion and the results showed a good agreement with the experimental data.

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“…However, in the case of direct current field, the non-conducting particles and cells experience only n-DEP [9]. DEP has been successfully implemented for cell sorting [10,11], sample preparation [12,13], cell detection [14,15,16], cell trapping [17], particle focusing [18,19], cell characterization [20] and cell patterning [21,22]. The dependence of the DEP force on the size and the electrical properties makes it possible for cell/ particle separation based on the size and electrical properties differences.…”

Section: Introductionmentioning

confidence: 99%

Continuous particle separation based on electrical properties using alternating current dielectrophoresis

Çetin

2009

Electrophoresis

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A design of a microchannel for continuous separation of particles based on alternating current dielectrophoretic is studied. The geometric design parameters are determined by analyzing the dielectrophoresis force field inside the microchannel corresponding to the most efficient separation. The trajectories of 5 and 10 mm spherical particles are derived analytically using Lagrangian tracking method to examine the feasibility and the effectiveness of the design. The effects of the mean flow velocity inside the channel and the applied voltage across the channel on the performance of the separation are also discussed.

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Feasibility study for cell electroporation detection and separation by means of dielectrophoresis

Cited by 46 publications

References 30 publications

Dielectrophoretic capture voltage spectrum for measurement of dielectric properties and separation of cancer cells

Dielectrophoretic capture voltage spectrum for measurement of dielectric properties and separation of cancer cells

Continuous particle separation by size via AC‐dielectrophoresis using a lab‐on‐a‐chip device with 3‐D electrodes

Continuous particle separation based on electrical properties using alternating current dielectrophoresis

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