Feasibility studies with lignin blocking additives in enhancing saccharification and cellulase recovery: Mutant UV-8 of T. verruculosus IIPC 324 a case study

Jain, Lavika; Kurmi, Akhilesh Kumar; Agrawal, Deepti

doi:10.1016/j.enzmictec.2018.07.003

Cited by 6 publications

(6 citation statements)

References 26 publications

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“…improvement (69% to 83%) in saccharification efficiency by Cellic CTec2 when acid pretreated sugarcane bagasse hydrolysis was carried out for 72h with and without surfactant addition [9]. The present data is far superior to the results obtained by Alhammad et al, who reported 19.2% improvement in enzymatic saccharification of 5% steam pretreated poplar when PEG 4000 was added at a concentration of 1% w/w after 96h of hydrolysis with Cellic CTec2 added at a concentration of 5% w/w basis [12].…”

Section: Resultsmentioning

confidence: 99%

“…In the upstream processing, the hydrolysis of cellulosic fraction involves the use of cellulase cocktail, a key bottleneck to the process. Several strategies have been employed in the state of the art, for reducing the overall cost of the cellulases by either developing novel enzyme cocktails [3], improvising pretreatment methods for high solid loadings [4], screening for low lignin binding enzymes [5,6], recycling and reusing the enzymes [7,8], enhanced saccharification using surfactants and proteins [9][10][11][12] or bioprospecting new microbes capable of consolidated bioprocessing [13].…”

Section: Introductionmentioning

confidence: 99%

“…In the 5C. All these studies were carried out by addition PEG 6000 at concentration of 0.3g/g AISL content, 7.5% substrate concentration and enzyme loading of 25mg protein/g cellulose content, based on our previous results [9].…”

Section: Introductionmentioning

confidence: 99%

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Augmented hydrolysis of acid pretreated sugarcane bagasse by PEG 6000 addition: a case study of Cellic CTec2 with recycling and reuse

Baral

Jain

Kurmi

et al. 2019

Bioprocess Biosyst Eng

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In an integrated lignocellulosic biorefinery, the cost associated with the "cellulases" and "longer duration of cellulose hydrolysis" represents the two most important bottlenecks. Thus to overcome these barriers, the present study aimed towards augmented hydrolysis of acid pretreated sugarcane bagasse within a short span of 16h using Cellic CTec2 by addition of PEG 6000. Addition of this surfactant not only enhanced glucose release by two fold within stipulated time, but aided in recovery of Cellic CTec2 which was further recycled and reused for second round of saccharification. During first round of hydrolysis, when Cellic CTec2 was loaded at 25mg protein /g cellulose content, it resulted in 76.24± 2.18% saccharification with a protein recovery of 58.4 ± 1.09%. Filtration through 50KDa PES membrane retained ~89% protein in 4.5 fold concentrated form and lead to simultaneous fractionation of ~70% glucose in the permeate. Later, the saccharification potential of recycled Cellic CTec2 was assessed for the second round of saccharification using two different approaches. Unfortified enzyme effectively hydrolysed 67% cellulose whereas 72% glucose release was observed with Cellic CTec2 fortified with 25% fresh protein top-up. Incorporating the use of the recycled enzyme in two-stage hydrolysis could effectively reduce the Cellic CTec2 loading from 25 to 16.8 mg protein/g cellulose. Further, 80% ethanol conversion efficiencies were achieved when glucose-rich permeate obtained after the first and second rounds of saccharification were evaluated using Saccharomyces cerevisiae MTCC 180.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Augmented hydrolysis of acid pretreated sugarcane bagasse by PEG 6000 addition: a case study of Cellic CTec2 with recycling and reuse

Baral

Jain

Kurmi

et al. 2019

Bioprocess Biosyst Eng

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“…Surfactants are of interest here due to their low cost and the fact that they maintain enzyme stability during hydrolysis. The enzymes can be recycled for several rounds [ 28 ]. Triton X-100, an amphiphilic nonionic surfactant, contains hydrophilic heads and hydrophobic tails.…”

Section: Introductionmentioning

confidence: 99%

“…These enzymatic cocktails contain cellulases and hemicellulases with a wide range of abilities to hydrolyze lignocellulosic biomass [ 32 ]. Triton X-100 was used because of its good lignin-binding ability [ 27 , 28 , 30 , 33 ]. Box-Behnken design and ANOVA analysis were used to create an experimental matrix, analyze and optimize models, respectively.…”

Section: Introductionmentioning

confidence: 99%

Interplays of enzyme, substrate, and surfactant on hydrolysis of native lignocellulosic biomass

et al. 2021

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Tracking enzyme, substrate, and surfactant interactions to reach maximum reducing sugar production during enzymatic hydrolysis of plant biomass may provide a better understanding of factors that limit the lignocellulosic material degradation in native rice straw. In this study, enzymes (Cellic Ctec2 cellulase and Cellic Htec2 xylanase) and Triton X-100 (surfactant) were used as biocatalysts for cellulose and xylan degradation and as a lignin blocking agent, respectively. The response surface model (R 2 = 0.99 and R 2 -adj = 0.97) indicated that Cellic Ctec2 cellulase (p < 0.0001) had significant impacts on reducing sugar production, whereas Cellic Htec2 xylanase and Triton X-100 had insignificant impacts on sugar yield. Although FTIR analysis suggested binding of Triton X-100 to lignin surfaces, the morphological observation by SEM revealed similar surface features (i.e., smooth surfaces with some pores) of rice straw irrespective of Triton X-100. The reducing sugar yields from substrate hydrolysis with or without the surfactant were comparable, suggesting similar exposure of polysaccharides accessible to the enzymes. The model analysis and chemical and structural evidence suggest that there would be no positive effects on enzymatic hydrolysis by blocking lignins with Triton X-100 if high lignin coverage exists in the substrate due to the limited availability of hydrolyzable polysaccharides.

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Exploring the flexibility of cellulase cocktail obtained from mutant UV-8 of Talaromyces verruculosus IIPC 324 in depolymerising multiple agro-industrial lignocellulosic feedstocks

Jain

Kurmi

Kumar

et al. 2020

International Journal of Biological Macromolecules

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Feasibility studies with lignin blocking additives in enhancing saccharification and cellulase recovery: Mutant UV-8 of T. verruculosus IIPC 324 a case study

Cited by 6 publications

References 26 publications

Augmented hydrolysis of acid pretreated sugarcane bagasse by PEG 6000 addition: a case study of Cellic CTec2 with recycling and reuse

Augmented hydrolysis of acid pretreated sugarcane bagasse by PEG 6000 addition: a case study of Cellic CTec2 with recycling and reuse

Interplays of enzyme, substrate, and surfactant on hydrolysis of native lignocellulosic biomass

Exploring the flexibility of cellulase cocktail obtained from mutant UV-8 of Talaromyces verruculosus IIPC 324 in depolymerising multiple agro-industrial lignocellulosic feedstocks

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