Feasibility of urinary microRNA profiling detection in intrahepatic cholestasis of pregnancy and its potential as a non-invasive biomarker

Ma, Li; Zhang, Xiaoqing; Zhou, Daxue; Cui, Yue; Deng, Linlin; Yang, Ting; Shao, Yong; Ding, Min

doi:10.1038/srep31535

Cited by 22 publications

(18 citation statements)

References 45 publications

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“…MMP2, MMP9, TIMP4, HDAC1, RELA, SP1, TP53, and other molecules were found to be the potential targets of hsa-miR-151a-3p and the gene products are the active players of ECM remodeling [26]. The microRNA hsa-miR-151a-3p was involved in cardiac hypertrophy, carcinogenesis and polycystic ovary [26]. But, the reports regarding the role of hsa-miR-151a-3p in human shoulder tendinopathies are limited in the literature.…”

Section: Discussionmentioning

confidence: 99%

“…Superoxide dismutase 2 (SOD2) was identified to be one of the direct targets of miR-382 suggesting their protective role in oxidative stress. The inhibition of miR-382 upregulated E-cadherin expression and altered the cellular phenotypes in diabetic kidneys [26, 29]. In cancer, miR-382 was shown to suppress osteosarcoma metastasis by targeting YB-1 oncogene [30, 31].…”

Section: Discussionmentioning

confidence: 99%

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MicroRNAs Associated with Shoulder Tendon Matrisome Disorganization in Glenohumeral Arthritis

et al. 2016

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The extracellular matrix (ECM) provides core support which is essential for the cell and tissue architectural development. The role of ECM in many pathological conditions has been well established and ECM-related abnormalities leading to serious consequences have been identified. Though much has been explored in regards to the role of ECM in soft tissue associated pathologies, very little is known about its role in inflammatory disorders in tendon. In this study, we performed microRNA (miRNA) expression analysis in the long head of the human shoulder biceps tendon to identify key genes whose expression was altered during inflammation in patients with glenohumeral arthritis. We identified differential regulation of matrix metalloproteinases (MMPs) that could be critical in collagen type replacement during tendinopathy. The miRNA profiling showed consistent results between the groups and revealed significant changes in the expression of seven different miRNAs in the inflamed tendons. Interestingly, all of these seven miRNAs were previously reported to have either a direct or indirect role in regulating the ECM organization in other pathological disorders. In addition, these miRNAs were also found to alter the expression levels of MMPs, which are the key matrix degrading enzymes associated with ECM-related abnormalities and pathologies. To our knowledge, this is the first report which identifies specific miRNAs associated with inflammation and the matrix reorganization in the tendons. Furthermore, the findings also support the potential role of these miRNAs in altering the collagen type ratio in the tendons during inflammation which is accompanied with differential expression of MMPs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

MicroRNAs Associated with Shoulder Tendon Matrisome Disorganization in Glenohumeral Arthritis

et al. 2016

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“…Among them, 15 miRNAs were upregulated (p < 0.05) and 9 were downregulated (p < 0.05) in the ICP group. 72 …”

Section: Obstetricsmentioning

confidence: 99%

Beyond circulating microRNA biomarkers: Urinary microRNAs in ovarian and breast cancer

et al. 2017

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Breast cancer is the most common malignancy in women worldwide, and ovarian cancer is the most lethal gynecological malignancy. Women carrying a BRCA1/2 mutation have a very high lifetime risk of developing breast and ovarian cancer. The only effective risk-reducing strategy in BRCA-mutated women is a prophylactic surgery with bilateral mastectomy and bilateral salpingo-oophorectomy. However, many women are reluctant to undergo these prophylactic surgeries due to a consequent mutilated body perception, unfulfilled family planning, and precocious menopause. In these patients, an effective screening strategy is available only for breast cancer, but it only consists in close radiological exams with a significant burden for the health system and a significant distress to the patients. No biomarkers have been shown to effectively detect breast and ovarian cancer at an early stage. MicroRNAs (miRNAs) are key regulatory molecules operating in a post-transcriptional regulation of gene expression. Aberrant expression of miRNAs has been documented in several pathological conditions, including solid tumors, suggesting their involvement in tumorigenesis. miRNAs can be detected in blood and urine and could be used as biomarkers in solid tumors. Encouraging results are emerging in gynecological malignancy as well, and suggest a different pattern of expression of miRNAs in biological fluids of breast and ovarian cancer patients as compared to healthy control. Aim of this study is to highlight the role of the urinary miRNAs which are specifically associated with cancer and to investigate their role in early diagnosis and in determining the prognosis in breast and ovarian cancer.

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“…This offers an exciting opportunity to develop easy, non-invasive and easy-to-repeat diagnostics for remote tissue-specific markers (Ma et al 2016) present in the urine. However, whether uEVs are actively secreted through the glomerular filtration barrier or through the epithelial cell lining of nephrons is not known, although this could involve many active mechanisms (for a variety of the mechanisms proposed, see Mulcahy et al 2014).…”

Section: Extracellular Vesiclesmentioning

confidence: 99%

Urinary extracellular vesicles. A promising shortcut to novel biomarker discoveries

Barreiro

Holthöfer

2017

Cell Tissue Res

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Proteomic and genomic techniques have reached full maturity and are providing unforeseen details for the comprehensive understanding of disease pathologies at a fraction of previous costs. However, for kidney diseases, many gaps in such information remain to inhibit major advances in the prevention, treatment and diagnostics of these devastating diseases, which have enormous global impact. The discovery of ubiquitous extracellular vesicles (EV) in all bodily fluids is rapidly increasing the fundamental knowledge of disease mechanisms and the ways in which cells communicate with distant locations in processes of cancer spread, immunological regulation, barrier functions and general modulation of cellular activity. In this review, we describe some of the most prominent research streams and findings utilizing urinary extracellular vesicles as highly versatile and dynamic tools with their extraordinary protein and small regulatory RNA species. While being a highly promising approach, the relatively young field of EV research suffers from a lack of adherence to strict standardization and carefully scrutinized methods for obtaining fully reproducible results. With the appropriate guidelines and standardization achieved, urine is foreseen as forming a unique, robust and easy route for determining accurate and personalized disease signatures and as providing highly useful early biomarkers of the disease pathology of the kidney and beyond.

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Feasibility of urinary microRNA profiling detection in intrahepatic cholestasis of pregnancy and its potential as a non-invasive biomarker

Cited by 22 publications

References 45 publications

MicroRNAs Associated with Shoulder Tendon Matrisome Disorganization in Glenohumeral Arthritis

MicroRNAs Associated with Shoulder Tendon Matrisome Disorganization in Glenohumeral Arthritis

Beyond circulating microRNA biomarkers: Urinary microRNAs in ovarian and breast cancer

Urinary extracellular vesicles. A promising shortcut to novel biomarker discoveries

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