Feasibility of thermophilic adenosine triphosphate-regeneration system using Thermus thermophilus polyphosphate kinase

Restiawaty, Elvi; Iwasa, Yoshihiro; Maya, Shohei; Honda, Kohsuke; Ômasa, Takeshi; Hirota, Ryuichi; Kuroda, Akio; Ohtake, Hisao

doi:10.1016/j.procbio.2011.05.021

Cited by 33 publications

(26 citation statements)

References 28 publications

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“…Polyphosphate with an average length of 60‐mer (polyP 60 ) was prepared according to methods described by Honda et al . The polyP 60 chain length was estimated by comparing its electrophoretic mobility with those of commercial standards (Polyphosphate glass 45, and 65 from Sigma), as described previously . To measure the reaction rate, the reaction mixture was incubated at 37 °C, and aliquots were collected at 0, 1, 2, and 5 min, respectively.…”

Section: Methodsmentioning

confidence: 99%

Class III Polyphosphate Kinase 2 Enzymes Catalyze the Pyrophosphorylation of Adenosine‐5′‐Monophosphate

et al. 2019

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Polyphosphate kinase 2 (PPK2) transfer phosphate from inorganic polyphosphate to nucleotides. According to their activity, PPK2 enzymes are classified into three groups. Among them, class III enzymes catalyze both the phosphorylation of nucleotide mono‐ to diphosphates and di‐ to triphosphates by using polyphosphate, which is a very inexpensive substrate. Therefore, class III enzymes are very attractive for use in biotechnological applications. Despite several studies on class III enzymes, a detailed mechanism of how phosphate is transferred from the polyphosphate to the nucleotide remains to be elucidated. Herein, it is reported that PPK2 class III enzymes from two different bacterial species catalyze the phosphorylation of adenosine mono‐ (AMP) into triphosphate (ATP) not only through step‐by‐step phosphorylation, but also by pyrophosphorylation. These are the first PPK2 enzymes that have been shown to possess polyphosphate‐dependent pyrophosphorylation activity.

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Section: Methodsmentioning

confidence: 99%

Class III Polyphosphate Kinase 2 Enzymes Catalyze the Pyrophosphorylation of Adenosine‐5′‐Monophosphate

et al. 2019

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“…Examples are the combination of a thermostable D ‐alanine‐ D ‐alanine ligase with PPK1 from the cyanobacterium Thermosynechococcus elongates 80 and the production of glycerol‐3‐phosphate with a glycerol kinase from Thermococcus kodakarensis and PPK1 from Thermus thermophilus. 81…”

Section: Emerging Enzymes and Phosphate Donors Used For In Vitro Amentioning

confidence: 99%

Emerging Enzymes for ATP Regeneration in Biocatalytic Processes

2015

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Adenosine-5'-triphosphate-dependent enzyme catalysed reactions are widespread in nature. Consequently, the enzymes involved have an intrinsic potential for use in syntheses of high value products. Although regeneration systems for ATP starting from adenosine-5'-diphosphate are available, certain limitations exist for both in vitro and in vivo applications requiring ATP regeneration from adenosine-5'-monophosphate, or adenosine. Following a short overview of the chemical and thermodynamic background, this Minireview focuses on emerging enzymes and methodologies for ATP regeneration. A large range of as yet unexploited reactions will be accessible with new, powerful, multistep ATP regeneration systems that use cheap phosphate donors and provide high longevity, compatibility, and robustness under process conditions. Their potential might go far beyond the direct use of ATP in enzymatic reactions; enzyme discovery, and engineering, as well as immobilisation strategies, will help to realise such systems.

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“…129,130 Furthermore, high concentrations of ATP inhibit some enzymes such as phosphofructokinase. 129 Several ATP regeneration systems have been created through the employment of biological agents including whole cells, organelles and enzymatic systems.…”

Section: Inorganic Polyphosphate As a Phosphoryl Donor Mutation In Dmentioning

confidence: 99%

“…129 Several ATP regeneration systems have been created through the employment of biological agents including whole cells, organelles and enzymatic systems. 130 Some of these enzymatic systems have been produced using several combinations of phosphate donors and enzymes, 131,132 such as acetyl phosphate (AcP) and acetate kinase, PEP with pyruvate kinase, and creatine phosphate (PC) combined with creatine kinase. ATP regeneration was applied to enzymatic synthesis of guanosine monophosphate (GMP), cytidine diphosphate-choline (CDP-choline) and other materials in the industry.…”

Section: Inorganic Polyphosphate As a Phosphoryl Donor Mutation In Dmentioning

confidence: 99%

“…131 Therefore, it is necessary to find a phosphoryl donor cheaper than ATP and, more important, that the final product does not inhibit the kinase. A very good candidate is the poly-P. 3,73,130 Inorganic poly-P is a linear polymer of up tens to hundreds of phosphates linked by high-energy phosphoanhydride bonds. 73,133,134 Figure 4.5.…”

Section: Inorganic Polyphosphate As a Phosphoryl Donor Mutation In Dmentioning

Feasibility of thermophilic adenosine triphosphate-regeneration system using Thermus thermophilus polyphosphate kinase

Cited by 33 publications

References 28 publications

Class III Polyphosphate Kinase 2 Enzymes Catalyze the Pyrophosphorylation of Adenosine‐5′‐Monophosphate

Class III Polyphosphate Kinase 2 Enzymes Catalyze the Pyrophosphorylation of Adenosine‐5′‐Monophosphate

Emerging Enzymes for ATP Regeneration in Biocatalytic Processes

Theoretical Studies of the Catalytic Mechanism of the Dihydroxyacetone Kinase

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