Feasibility of peripheral blood stem cell (PBSC) and peripheral blood mononuclear cell (PBMNC) separation in children with a body weight below 20 KG

Urban, C; Schwinger, Wolfgang; Benesch, Martin; Lackner, Herwig; Kerbl, Reinhold; Gilli, R; Pätzold, Ursula; Burdach, S

doi:10.1002/(sici)1096-911x(199708)29:2<115::aid-mpo9>3.0.co;2-h

Cited by 23 publications

(12 citation statements)

References 11 publications

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“…The tolerance of the extracorporeal circuit, even in very small children, can be good. In our series, as in series reported by other authors, 15,16 apheresis induced few side effects: two serious (femoral AVF and haemodynamic disorders) and five mild transient side effects.…”

Section: Discussionsupporting

confidence: 88%

“…10,12,[14][15][16] However, in many cases, this initial systematic transfusion can be avoided even in low-weight children. With reference to initial haemoglobin values, fewer children will be transfused with this method, even when repeated collections are required, than if each apheresis is systematically primed with red blood cells.…”

Section: Discussionmentioning

confidence: 99%

“…Our results show that a normal volume apheresis procedure (ie o3 Â BV) can be successfully used, while some authors prefer large-volume apheresis (6 Â BV) to increase absolute yields of CD34+. [16][17][18][19] In our experience, despite their low weight, insertion of a femoral catheter can be avoided in 43% of children and use of a peripheral vein was sufficient. Some authors 12 use a similar technique but, in most of their cases, regardless of The morbidity following catheter insertion is low, 20 but our results show that the child's low weight does not systematically indicate the need for central venous catheter and, with experience, an arm or forearm vein can be used for return of processed blood, even in small children.…”

Section: Discussionmentioning

confidence: 99%

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Peripheral blood stem cell collection in 24 low-weight infants: experience of a single centre

Orbach

Hojjat-Assari

Doz

et al. 2003

Bone Marrow Transplant

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Summary:Peripheral blood stem cells (PBSC) harvest may be difficult in young children. Extracorporeal separator line priming by red blood cells is usually required to improve haemodynamic tolerance and efficacy of collection. We present our experience with 24 children weighing less than 15 kg treated between January 1997 and September 1999, in whom we tried to avoid systematic blood priming. The median age and weight at the time of apheresis were 2.4 years and 12 kg, respectively. A total of 48 PBSC were performed. When haemoglobin was less than 12 g/dl, packed red cells were transfused before collection (40% of aphereses). The median cell yield per apheresis was 7.1 (2.2-30.6) Â 10 6 /kg CD34 + cells and 16.0 (3.3-44.3) Â 10 5 CFU-GM/kg. Initial collection failed in three cases. Four children required an additional haematopoietic progenitor mobilization. This procedure allowed PBSC collection without transfusion in 37.5% of children, and was safe (two serious and five mild transient side effects) and effective (median CD34 + cells collected per child: 7.1 Â 10 6 /kg (4.6-30.6) and CFU-GM: 15.1 Â 10 5 /kg (4.7-44.3)). Despite their low weight, insertion of a femoral catheter was avoided in 43% of children.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Peripheral blood stem cell collection in 24 low-weight infants: experience of a single centre

Orbach

Hojjat-Assari

Doz

et al. 2003

Bone Marrow Transplant

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show abstract

“…The duration of the procedure generally ranges 80± 500 min in relation to the median number of patient's blood volume processed, for reduction of apheresis number. The collection volume can be reduced or increased according to the patient's needs and leukapheresis can be repeated on consecutive days [12,13,17].…”

mentioning

confidence: 99%

“…Recent reports indicate that a wide range of CD34 + cell numbers causing various but successful early or late neutrophil and or platelet recovery and engraftment can be tried. Concerning the number of CD34 + cells, a minimum 3.0 £ 10 6 /kg is estimated to be adequate for successful autografting even in a double tranplantation setting for children weighting less than 20 kg [12,19].…”

mentioning

confidence: 99%