A field experiment was conducted at the farmer's field, Gayeshpur, Nadia, West Bengal during 2013-15 to study the effect of different bio-fertilizers on yield, fruit quality and leaf mineral composition of guava cv. L-49. The experiment was implemented using a randomized complete-block design with three replicates. Ten treatments of different bio-fertilizers alone and in combinations along with one chemical and absolute control i.e. no fertilization treatment were applied on 6 years old guava plant. Among different treatments of bio fertilizers, Azospirillum brasilense + AMF (Arbuscular mycorhizal fungi) showed highest (56.30%) fruit retention and maximum fruit yield (41.3 kg plant -1