Feasibility of Hydrocyclones for Combined Cleaning of Circulation and Waste Water

Dikov, V. A.; Sukhanov, D. E.

doi:10.1007/s10556-014-9806-0

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hydrocyclones, which have been used for decades in several industries (Bicalho et al, 2012; Dikov & Sukhanov, 2014; Grommers et al, 2004; Oats et al, 2010; Zemanek, 1973) have also been investigated for their potential as cell retention devices (Deckwer et al, 2005; Elsayed et al, 2006; Medronho et al, 2005; Ortega‐Rivas, 2004; Pinto et al, 2008; Schröder et al, 2010). Hydrocyclones generate an internal centrifugal force as the fluid circulates through the device at high flow rates, separating cells from bulk culture fluid.…”

Section: Introductionmentioning

confidence: 99%

Hydrocyclones as cell retention devices for an N‐1 perfusion bioreactor linked to a continuous‐flow stirred tank production bioreactor

Kundu

Hiller

2021

Biotech & Bioengineering

View full text Add to dashboard Cite

A continuous Chinese hamster ovary (CHO) cell culture process comprised of a highly proliferative N‐1 perfusion bioreactor utilizing a hydrocyclone as a cell retention device linked to a production continuous‐flow stirred tank reactor (CSTR) is presented. The overflow stream from the hydrocyclone, which is only partially depleted of cells, provides a continuous source of high viability cells from the N‐1 perfusion bioreactor to the 5–20 times larger CSTR. Under steady‐state conditions, this linked‐bioreactor system achieved a peak volumetric productivity of 0.96 g/L/day, twofold higher than the optimized fed‐batch process. The linked bioreactor system using a hydrocyclone was also shown to be 1.8–3.1 times more productive than a dual, cascading CSTR system without cell retention.

show abstract