“…Hydrocyclones, which have been used for decades in several industries (Bicalho et al, 2012; Dikov & Sukhanov, 2014; Grommers et al, 2004; Oats et al, 2010; Zemanek, 1973) have also been investigated for their potential as cell retention devices (Deckwer et al, 2005; Elsayed et al, 2006; Medronho et al, 2005; Ortega‐Rivas, 2004; Pinto et al, 2008; Schröder et al, 2010). Hydrocyclones generate an internal centrifugal force as the fluid circulates through the device at high flow rates, separating cells from bulk culture fluid.…”