Abstract:The feasibility of a high-loaded membrane bioreactor to improve methane recovery from sewage was investigated. Although the process needs further optimization, it already is feasible to recover at least 35% of the sewage COD. Important aspects for further research are the occurrence of membrane fouling, and the optimum process conditions for bioflocculation, i.e. the proper SRT/HRT ratio, dissolved oxygen concentration and shear and overall energy production and consumption under optimised conditions.
“…1). This was in line with earlier studies about HLMBRs (Akanyeti et al 2010;Faust et al 2014a). Enhanced bioflocculation was positively correlated to the increased concentration of sludge-bound EPS at longer SRTs when five HLMBRs were operated at various SRTs between 0.125 and 1 day (Faust et al 2014a).…”
Section: Bioflocculation At Different Srtssupporting
confidence: 91%
“…Flocculation efficiency increased from 49 % in R0.125 to 84 % in R0.5 and to 94 % in R1. This is in line with earlier studies investigating the bioflocculation process in HL-MBRs (Akanyeti et al 2010;Faust et al 2014a). A previous research (Faust et al 2014a) showed that the increase of bioflocculation at longer SRTs was accompanied by higher sludge concentration of bound EPS, and it was concluded that EPS, in particular EPS-proteins, govern the bioflocculation process in HLMBRs.…”
Section: Cod Fractionssupporting
confidence: 90%
“…With such a HL-MBR, sewage organic matter can be concentrated and in this manner facilitates subsequent energy or chemical production from this organic matter (Akanyeti et al 2010;Hernández Leal et al 2010).…”
High-loaded membrane bioreactors (HL-MBRs), i.e., bioreactors equipped with a membrane for biomass retention and operated at extremely short sludge and hydraulic retention times, can concentrate sewage organic matter to facilitate subsequent energy and chemical recovery from these organics. Bioflocculation, accomplished by microorganisms that produce extracellular polymers, is a very important mechanism in these reactors. Bacterial diversity of the sludge and supernatant fraction of HL-MBRs operated at very short sludge retention times (0.125, 0.5, and 1 day) were determined using a PCR-denaturing gradient gel electrophoresis (DGGE) and clone library approach and compared to the diversity in sewage. Already at a sludge retention time (SRT) of 0.125 day, a distinct bacterial community developed compared to the community in sewage. Bioflocculation, however, was low and the majority of the bacteria, especially Arcobacter, were present in the supernatant fraction. Upon increasing SRT from 0.125 to 1 day, a much stronger bioflocculation was accompanied by an increased abundance of Bacteroidetes in the (solid) sludge fraction: 27.5 % at an SRT of 0.5 day and 46.4 % at an SRT of 1 day. Furthermore, cluster analysis of DGGE profiles revealed that the bacterial community structure in the sludge was different from that in the supernatant. To localize specific bacterial classes in the sludge flocs, fluorescence in situ hybridization (FISH) was carried out with three different bacterial probes. This showed that Betaproteobacteria formed clusters in the sludge flocs whereas Alphaproteobacteria and Gammaproteobacteria were mainly present as single cells.
“…1). This was in line with earlier studies about HLMBRs (Akanyeti et al 2010;Faust et al 2014a). Enhanced bioflocculation was positively correlated to the increased concentration of sludge-bound EPS at longer SRTs when five HLMBRs were operated at various SRTs between 0.125 and 1 day (Faust et al 2014a).…”
Section: Bioflocculation At Different Srtssupporting
confidence: 91%
“…Flocculation efficiency increased from 49 % in R0.125 to 84 % in R0.5 and to 94 % in R1. This is in line with earlier studies investigating the bioflocculation process in HL-MBRs (Akanyeti et al 2010;Faust et al 2014a). A previous research (Faust et al 2014a) showed that the increase of bioflocculation at longer SRTs was accompanied by higher sludge concentration of bound EPS, and it was concluded that EPS, in particular EPS-proteins, govern the bioflocculation process in HLMBRs.…”
Section: Cod Fractionssupporting
confidence: 90%
“…With such a HL-MBR, sewage organic matter can be concentrated and in this manner facilitates subsequent energy or chemical production from this organic matter (Akanyeti et al 2010;Hernández Leal et al 2010).…”
High-loaded membrane bioreactors (HL-MBRs), i.e., bioreactors equipped with a membrane for biomass retention and operated at extremely short sludge and hydraulic retention times, can concentrate sewage organic matter to facilitate subsequent energy and chemical recovery from these organics. Bioflocculation, accomplished by microorganisms that produce extracellular polymers, is a very important mechanism in these reactors. Bacterial diversity of the sludge and supernatant fraction of HL-MBRs operated at very short sludge retention times (0.125, 0.5, and 1 day) were determined using a PCR-denaturing gradient gel electrophoresis (DGGE) and clone library approach and compared to the diversity in sewage. Already at a sludge retention time (SRT) of 0.125 day, a distinct bacterial community developed compared to the community in sewage. Bioflocculation, however, was low and the majority of the bacteria, especially Arcobacter, were present in the supernatant fraction. Upon increasing SRT from 0.125 to 1 day, a much stronger bioflocculation was accompanied by an increased abundance of Bacteroidetes in the (solid) sludge fraction: 27.5 % at an SRT of 0.5 day and 46.4 % at an SRT of 1 day. Furthermore, cluster analysis of DGGE profiles revealed that the bacterial community structure in the sludge was different from that in the supernatant. To localize specific bacterial classes in the sludge flocs, fluorescence in situ hybridization (FISH) was carried out with three different bacterial probes. This showed that Betaproteobacteria formed clusters in the sludge flocs whereas Alphaproteobacteria and Gammaproteobacteria were mainly present as single cells.
“…These characteristics prevent the direct production of valuable resources from organic matter, such as methane or volatile fatty acids (VFA), and makes pre-treatment to concentrate the organic matter necessary. Aerobic bioflocculation of raw sewage in a high-loaded membrane bioreactor (HL-MBR) is a promising technique to accomplish such a concentration step, while at the same time it can produce a water quality that is fit for reuse (Akanyeti et al, 2010;Faust et al, 2014 Often anaerobic digestion is applied to reduce the amount of primary sludge (PS) and secondary activated sludge (AS) and to produce methane from these solids (Lettinga, 1995). This process consists of four subsequent steps: hydrolysis, acidogenesis, acetogenesis and methanogenesis.…”
Section: Introductionmentioning
confidence: 99%
“…This result is in line with the study by Ferreiro and Soto (2003), who found a specific VFA production of 170 -370 mg COD/g VSS of PS and a VFA composition of 37-60% acetate, 30-55% propionate and 8-20% butyrate. Akanyeti et al (2010) reported that with a combination of aerobic bioflocculation and subsequent anaerobic digestion at least 35% of sewage COD can be converted to methane. This yield is much higher than a methane recovery of 18% when PS and/or a mixture of PS and AS are digested (Cao, 2011).…”
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