Feasibility of a breath test with a substrate of natural <sup>13</sup>C‐abundance and isotope‐selective non‐dispersive infrared spectrometry: A preliminary study

Jonderko, Krzysztof; Kasicka-Jonderko, Anna; Syrkiewicz-Trepiak, Danuta; Błońska‐Fajfrowska, Barbara

doi:10.1111/j.1440-1746.2005.03920.x

Cited by 16 publications

(15 citation statements)

References 17 publications

(48 reference statements)

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“…During an introductory interview, the subjects were instructed not to eat any food of naturally increased 13 C content, such as products made of maize, cane sugar, pineapple, and kiwi fruit, for 48 h preceding the examination [17,18].…”

Section: Subjectsmentioning

confidence: 99%

Exact profiles of ¹³CO₂ recovery in breath air after per oral administration of [¹³C]methacetin in two groups of different ages

Kasicka-Jonderko

Jonderko

Chabior

et al. 2008

Isotopes in Environmental and Health Studies

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The aim of this study is to determine if age is a factor influencing the results of a [(13)C]methacetin breath test ((13)C-MBT). Two groups of healthy volunteers, each comprising six men and six women, but differing in average age (Y=young, 25.1+/-0.6 years, MA=middle-aged;, 46.0+/-2.1 years) orally took 75 mg [(13)C]methacetin. Samples of expiratory air for (13)CO(2) measurement were collected up to 48 h after intake of the substrate. A maximum momentary (13)CO(2) breath exhalation of 37.0+/-2.6%dose/h was observed at 18 min (median, range: 9-30 min) in the young subjects and of 38.4+/-2.5%dose/h at 18 min (median, range: 12-30 min) in the middle-age volunteers. The cumulative (13)C elimination in expiratory air was statistically significantly higher in the MA compared with the Y group as from 75 min up to 180 min, indicating a greater microsomal metabolic efficiency of the liver in the middle-aged healthy subjects. Gender, use of hormonal contraception, cigarette smoking, or body mass index did not modify the age-related effect on the cumulative (13)C elimination in breath air. The study results imply a necessity of composing control groups well matched with regard to the age structure for a proper interpretation of clinical (13)C-MBT results.

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Section: Subjectsmentioning

confidence: 99%

Exact profiles of ¹³CO₂ recovery in breath air after per oral administration of [¹³C]methacetin in two groups of different ages

Kasicka-Jonderko

Jonderko

Chabior

et al. 2008

Isotopes in Environmental and Health Studies

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“…Studies investigating the oxidative fates of exogenous nutrients orally administer some 13 C-enriched material (e.g., often nutrients derived from C4 plants) and report the extent to which the δ 13 C increases in the breath (Massicotte et al, 1986;Peronnet et al, 1990;Loser et al, 1997;Jonderko et al, 2005;McCue, 2011). If the background 13 C-value of the breath of participants is not constant as it tends to be in postabsorptive, non-exercising participants, also as shown in this study, then some correction or offset must be made to account the exercise-induced changes in δ 13 C accurately model the effect of the exogenous nutrient on the breath δ 13 C.…”

Section: δ 13 C In the Exhaled Breathmentioning

confidence: 99%

The magnitude of the naturally occurring isotopic enrichment of 13C in exhaled CO2 is directly proportional to exercise intensity in humans

McCue

Passement

Rodriguez

2015

Comparative Biochemistry and Physiology Part A: Molecular &

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“…Researchers and clinicians who routinely use free 13 C-amino acid tracers in lieu of proper 13 C-protein tracers (including the authors of the present paper) openly acknowledge that this practice violates the primary assumption of tracer methodology. However, these individuals justify their actions by citing the fact that large amounts (> 100 grams) of nutritionally complete, artificially enriched, 13 C-labeled proteins remain commercially unavailable (Berthold et al, 2011;Braden, 2010;Fromentin et al, 2011a;Jonderko et al, 2005).…”

Section: Amino Acid Tracers Are Not the Same As Protein Tracersmentioning

confidence: 99%

Hens Produce Artificially Enriched 13C Egg Proteins for Metabolic Tracer Studies

McCue

Arquisola²,

Albach³

et al. 2013

IJB

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Clinicians and researchers studying protein metabolism in vivo, typically use isotopically-labeled free amino acids as metabolic tracers rather than isotopically-labeled proteins because such proteins are commercially unavailable. However, the use of free amino acids in lieu of protein tracers violates the critical assumption that tracer molecules undergo the identical biochemical reactions as the tracee molecules of interest. To address this problem we synthesized 13 C-labeled proteins using egg laying hens and investigated the relationship between tracer dose and method of delivery on 13 C-protein production. We enriched hens with one of two isotope tracers ( 13 C-1-leucine or a uniformly labeled 13 C-amino acid mixture) mixed in their food or dissolved in their drinking water at different dosing levels (86-432 mg day -1 ). The recovery of 13 C in egg white proteins of the hen fed 13 C-leucine ranged from 14% to 21%; recovery rates were highest at the lowest dosing level. At the highest dosing level egg whites were enriched more than 150‰ above background levels of 13 C. The time required for half maximal 13 C enrichment depended chiefly on the mode of tracer administration, and ranged from 2.5 days for 13 C-leucine dissolved in water to 4.9 days for 13 C-leucine mixed in food. Relative rates of 13 C recovery in the egg protein were lowest for hens fed the uniformly 13 C labeled amino acid mixture, presumably because of the high proportion of nonessential amino acids. The time required for the 13 C-enrichment in eggs to return to background levels at the end of the enrichment period was about twice that required to initially reach isotopic equilibrium with the diet, indicating significant biochemical discrimination of endogenous 13 C amino acids. We conclude that delivering small amounts of 13 C amino acid tracers in the drinking water of hens is the most effective way to produce 13 C-enriched proteins to for tracer studies that do not require δ 13 C-enrichment above 200‰.

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Feasibility of a breath test with a substrate of natural ¹³C‐abundance and isotope‐selective non‐dispersive infrared spectrometry: A preliminary study

Abstract: The obtained proof of technical feasibility of a breath test with the use of naturally (13)C-enriched starch and NDIRS provides background for future research on the clinical usefulness of this method for a non-invasive assessment of the pancreatic exocrine function.

Cited by 16 publications

References 17 publications

Exact profiles of ¹³CO₂ recovery in breath air after per oral administration of [¹³C]methacetin in two groups of different ages

Exact profiles of ¹³CO₂ recovery in breath air after per oral administration of [¹³C]methacetin in two groups of different ages

The magnitude of the naturally occurring isotopic enrichment of 13C in exhaled CO2 is directly proportional to exercise intensity in humans

Hens Produce Artificially Enriched 13C Egg Proteins for Metabolic Tracer Studies

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Feasibility of a breath test with a substrate of natural 13C‐abundance and isotope‐selective non‐dispersive infrared spectrometry: A preliminary study

Abstract: The obtained proof of technical feasibility of a breath test with the use of naturally (13)C-enriched starch and NDIRS provides background for future research on the clinical usefulness of this method for a non-invasive assessment of the pancreatic exocrine function.

Cited by 16 publications

References 17 publications

Exact profiles of 13CO2 recovery in breath air after per oral administration of [13C]methacetin in two groups of different ages

Exact profiles of 13CO2 recovery in breath air after per oral administration of [13C]methacetin in two groups of different ages

The magnitude of the naturally occurring isotopic enrichment of 13C in exhaled CO2 is directly proportional to exercise intensity in humans

Hens Produce Artificially Enriched 13C Egg Proteins for Metabolic Tracer Studies

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Feasibility of a breath test with a substrate of natural ¹³C‐abundance and isotope‐selective non‐dispersive infrared spectrometry: A preliminary study

Exact profiles of ¹³CO₂ recovery in breath air after per oral administration of [¹³C]methacetin in two groups of different ages

Exact profiles of ¹³CO₂ recovery in breath air after per oral administration of [¹³C]methacetin in two groups of different ages