In this work, a boronate affinity-functionalized hierarchical
mesoporous
metal–organic framework adsorbent with boronate sites only
in the small mesopore has been structured based on UiO-66@Fe3O4. The introduction of large mesopores in the adsorbent
can promote the diffusion of small cis-diol-containing
compounds (cis-diols) into small mesopore channels,
and the removal of the adsorption sites on the external surface of
materials and in large mesopores can enhance the size-exclusion effect
of the adsorbent. In addition, the adsorbent has faster adsorption
kinetics and excellent selectivity to small cis-diols.
Finally, a magnetic dispersive solid-phase extraction coupled with
high-performance liquid chromatography was established for the enrichment
and detection of nucleotides in plasma. Four nucleotides achieve the
recoveries from 93.25 to 118.79%, the limits of detection from 0.35
to 1.26 ng·mL–1, and the intra-day and inter-day
relative standard deviations of less than 10.2%. In conclusion, this
method can be directly used for the detection of small cis-diol targets in complex biological samples without protein precipitation
prior to the extraction.