Fe deficiency induces phosphorylation and translocation of Lhcb1 in barley thylakoid membranes

Abstract

a b s t r a c tHvLhcb1 a major light-harvesting chlorophyll a/b-binding protein in barley, is a critical player in sustainable growth under Fe deficiency. Here, we demonstrate that Fe deficiency induces phosphorylation of HvLhcb1 proteins leading to their migration from grana stacks to stroma thylakoid membranes. HvLhcb1 remained phosphorylated even in the dark and apparently independently of state transition, which represents a mechanism for short-term acclimation. Our data suggest that the constitutive phosphorylation-triggered translocation of HvLhcb1 under Fe deficiency contributes to optimization of the excitation balance between photosystem II and photosystem I, the latter of which is a main target of Fe deficiency.

Catechol-Loading Nanofibrous Membranes for Eco-Friendly Iron Nutrition of Plants

“…Depending on the stressing cause, the presence and number of PGs vary, as well as their association with and connection to thylakoid membranes [63]. PGs have been related to stresses, like high light, Fe-deficiency, salt concentration, and heavy metals [64,65,66]. In the present study, PGs were present in both leaf and root cells of plants from all treatments and displayed different types of structures assorted distinctly between the treatments and might be related to diverse functions or degree of maturation (Figure 5A–C green arrows and yellow arrowheads) [63,67].…”

Quantitative Phosphoproteomic and Physiological Analyses Provide Insights into the Formation of the Variegated Leaf in Catalpa fargesii

“…In our study, in addition to Thr 23, other phosphorylation sites of LHCB1 were all downregulated in the yellow sectors (Figure 9A). Studies have shown that the phosphorylation of LHCB1 is essential for optimizing the photosystem balance under changing environments [25,26]. The different phosphorylation sites of LHCB1 might play different roles in energy balance (Figure 9A).…”

Enhancement of Photosynthetic Iron-Use Efficiency Is an Important Trait of Hordeum vulgare for Adaptation of Photosystems to Iron Deficiency

“…Furthermore, prolonged Fe-deficiency (more than two weeks) induces non-photochemical quenching (NPQ), in which the excess-absorbed light energy is dissipated into heat, with the phosphorylation and monomerization of HvLhcb1, a major light-harvesting antenna protein of PSII. This NPQ induction results in protection from the photo-inhibitory effect caused by Fe-deficiency [13,18]. We propose that these features allow barley to sustain photosynthesis under long-term Fe-deficient conditions.…”