Abstract:Fluorescence microscopy is a standard tool in molecular biophysics, but even the best resolution obtained by diffraction-limited conventional optical techniques misses the molecular level by two orders of magnitude. In order to overcome the classical diffraction limit, several sub-diffraction resolution imaging methods have been introduced so far. Direct stochastic optical reconstruction microscopy (dSTORM) (1) and PAINT (points accumulation for imaging in nanoscale topography) (2) have the potential to shed l… Show more
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