FCD1 encoding protein homologous to cellobiose: Quinone oxidoreductase in Fusarium oxysporum

Purification and Characterization of a Carbohydrate:Acceptor Oxidoreductase fromParaconiothyriumsp. That Produces Lactobionic Acid Efficiently

“…CDH and CBQ had similar amino acid sequences. [13][14][15] Hence CDH and CBQ are generally classified into the same group. Since there is no homology in amino acid sequence between the former group (COX and GOOX) and the latter (CBQ and CDH), we propose a new classification that divides the enzymes into two groups: the COX and GOOX to COX group, and the CDH and CBQ to CDH groups.…”

“…COX and GOOX show no sequence homology to CBQ or CDH, whereas the amino acid sequences of CBQ and CDH are similar. 10,[12][13][14][15] These results indicate that COX and GOOX are different enzymes from CBQ and CDH, and that CBQ and CDH belong to the same group. On the other hand, no homology between COX and GOOX has been proposed.…”

“…10,12) PCOX showed no homology to those of CDH and CBQ from Fusarium oxysporum, as discussed below. 7,14,15,20) Effects of pH and temperature The effects of pH and temperature on the activity and stability of the enzyme were measured (Fig. 4).…”

GMC oxidoreductase, a highly expressed protein in a potent biocontrol agent Fusarium oxysporum Cong:1-2, is dispensable for biocontrol activity

“…Multiple alignment, phylogenetic analysis, and construction of a phylogenetic tree were performed using the ClustalX 1.81 (Saiou and Nei, 1987;Thompson et al, 1997), MEGA 3.1 (Kumar et al, 2004), and Phylip v. 3.63 (Felsenstein, 1981(Felsenstein, , 1996Fitch, 1977) software following previously described methods (Kawabe et al, 2006).…”

“…A branch with a shaded triangle indicates the putative protein in F. oxysporum including ODX1 (this study) and FCD1 (Kawabe et al, 2006). Colored circles indicate the determined or predicted function of each protein in the tree: green, versicolorin B synthase; blue, cellobiose dehydrogenase; brown, long-chain fatty acid alcohol oxidase; orange, alcohol oxidase; red, aryl alcohol oxidase; yellow, glucose oxidase.…”