Fc receptors on rabbit lymphocytes Existence of receptors for IgG antibody complexed with antigen; conditions for its detection

Bast, Bert J.E.G.; Manten-Slingerland, Ria; Roholl, P. J. M.; Meyling, F. Gmelig; Ballieux, R. E.

doi:10.1002/eji.1830100307

Cited by 10 publications

(2 citation statements)

References 37 publications

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“…To remove FcvR' cells, washed ox erythrocytes (E) were coated (EA) with a nonagglutinating dilution of rabbit IgG anti-E, washed, and mixed with BM cells at a ratio of 20 EA per BM cell [11]. The cell mixture was pelleted at 400 g for 10 min, incubated for 1 h on ice, gently resuspended and stained with euchrysine dye.…”

Section: Rosette-forming Cellsmentioning

confidence: 99%

Differential Activities of Rabbit Bone Marrow Suppressor Cells

Soderberg¹

1984

Int Arch Allergy Immunol

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A population of nonadherent Fcγ-receptor-bearing (FcγR⁺) suppressor cells in normal rabbit bone marrow (BM) which inhibited the constitutive proliferation of other BM cells has been previously described. Suppression was by blocking the release of a soluble growth-promoting factor. In the present report, it was found that the FcγR⁺ BM suppressor cells, which were suppressive to background proliferation, were not suppressive to lymphocyte activation by immune complexes (IC). Adherent BM cells showed suppressive activity toward IC stimulation, but none toward constitutive BM proliferation. IC-induced proliferation was enhanced by the soluble growth factor, but only in the absence of adherent cells. Adherent cells did not affect growth factor enhancement of constitutive proliferation, suggesting that different cell populations were affected. Depletion of the FcγR⁺ suppressor cells promoted growth of cells which subsequently developed FcγR⁺, increasing the possibility of interaction with IC, which also preferentially induced FcγR⁺ cells.

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Section: Rosette-forming Cellsmentioning

confidence: 99%

Differential Activities of Rabbit Bone Marrow Suppressor Cells

Soderberg¹

1984

Int Arch Allergy Immunol

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“…To remove FcyR' cells, washed ox erythrocytes (E) were coated (EA) with a nonagglutinating dilu tion of rabbit IgG anti-E, washed, and mixed with BM cells at a ra tio of 20 EA per BM cell [11]. The cell mixture was pelleted at 400 g for 10 min, incubated for I h on ice, gently resuspended and stained with euchrysine dye.…”

Section: Rosette-forming Cells (Rfc)mentioning

confidence: 99%

Regulation of Constitutive Bone Marrow Cell Proliferation by Bone Marrow Suppressor Cells

Soderberg¹

1984

Int Arch Allergy Immunol

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Bone marrow (BM) cells have previously been shown to suppress specific immune responses of cells from peripheral lymphoid organs. The present report describes a suppressor cell present in normal rabbit BM, which regulated the constitutive proliferation of other BM cells. The suppressor cells were Fcγ-receptor-positive (FcγR⁺) complement-receptor-negative, and nonadherent or weakly adherent. Similar suppressive activity was not detected among rabbit spleen cells. Removal of FcγR⁺ suppressor cells allowed greater than 10-fold increases in the proliferation of FcγR^–– BM cells. Addition of FcγR⁺ BM cells to FcγR^–– cells efficiently blocked proliferation. The suppressor cells acted by inhibiting the elaboration of a soluble growth-promoting factor by cells in the FcγR^–– population. The growth-promoting factor enhanced proliferation of unseparated rabbit BM cells and peripheral blood mononuclear cells.

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Surface Ig on rabbit lymphocytes Rabbit B and T cells are distinct populations

Bast¹,

Catty

Manten-Slingerland³

et al. 1979

Eur J Immunol

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Rabbit peripheral blood lymphocytes (PBL) were analyzed by immunofluorescence using anti-T cell conjugates and anti-Fab, anti-a1 allotype, anti-IgM and anti-IgA conjugates. In addition, T cells were demonstrated by rosetting with papain-treated homologous erythrocytes. Control experiments, using acid treatment and incubation at 37 degrees C for 18 h after or without pronase treatment, revealed the endogenous origin of all surface determinants tested. A good correlation was found between results obtained with the two anti-T cell conjugates used and the T rosette test on PBL and on lymphoid cells isolated from various organs. In lymphocytes isolated from peripheral blood and from various lymphoid organs, the percentages of T and B cells were respectively 45 and 38 for PBL, 10 and 46 for bone marrow, 27 and 31 for appendix, 40 and 45 for spleen, 42 and 46 for Peyer's patches, 96 and 0.3 for thymus and 70 and 16 for peripheral lymph nodes. The percentage of "null" cells in lymphocyte populations derived from bone marrow and appendix is rather high. The final percentages of T and B cells in rabbit PBL depend to a significant extent on the method of isolation, especially isolation by Ficoll-Hypaque centrifugation results in a depletion of T cells. Moreover, a rather impure lymphoid cell suspension is obtained. In double incubation experiments, T cells (as defined by T cell antigen(s) or rosette formation) and B cells (Fab-bearing cells) were entirely different subpopulations. Allotypes of the a locus could not be detected on the surface of T cells. The results are discussed with respect to genetic coding of antigen receptors on B and T cells.

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Fc receptors on rabbit lymphocytes Existence of receptors for IgG antibody complexed with antigen; conditions for its detection

Cited by 10 publications

References 37 publications

Differential Activities of Rabbit Bone Marrow Suppressor Cells

Differential Activities of Rabbit Bone Marrow Suppressor Cells

Regulation of Constitutive Bone Marrow Cell Proliferation by Bone Marrow Suppressor Cells

Surface Ig on rabbit lymphocytes Rabbit B and T cells are distinct populations

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