Fc engineering by monoclonal mammalian cell display for improved affinity and selectivity towards FcγRs
Zening Wang,
Minhyo Kang,
Afshin Ebrahimpour
et al.
Abstract:Fc optimization can significantly enhance therapeutic efficacy of monoclonal antibodies. However, existing Fc engineering approaches are sub-optimal with noted limitations, such as inappropriate glycosylation, polyclonal libraries, and utilizing fragment but not full-length IgG display. Applying cell cycle arrested recombinase-mediated cassette exchange, this study constructed high-quality monoclonal Fc libraries in CHO cells, displayed full-length IgG on cell surface, and preformed ratiometric fluorescence ac… Show more
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