Fc‐binding molecules specific for human IgG1 and IgG3 are present in <i>Echinococcus granulosus</i> protoscoleces

Baz, Adriana; Carol, Hernán; Marco, Marta; Casabó, Lucía; Jones, Frances M.; Dunne, David W.; Nieto, Alberto

doi:10.1046/j.1365-3024.1998.00147.x

Cited by 10 publications

(6 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, early T-independent antibody responses could be a common phenomenon in experimental infection by Echinococcus spp . Moreover, regarding the isotype restriction reported here it is interesting to note that tegument and suckers of E. granulosus protoscoleces have Fc-like receptors able to bind human IgG1 and IgG3 antibodies trough their specific Fc portions [33]. Such isotypes structurally and functionally correspond to murine IgG2a and IgG2b, respectively [34]; and therefore local IgG2b specific induction in early stages of infection could be seen as a possible evasion strategy exploited by E. granulosus .…”

Section: Discussionmentioning

confidence: 66%

Early Peritoneal Immune Response during Echinococcus granulosus Establishment Displays a Biphasic Behavior

et al. 2011

View full text Add to dashboard Cite

BackgroundCystic echinococcosis is a worldwide distributed helminth zoonosis caused by the larval stage of Echinococcus granulosus. Human secondary cystic echinococcosis is caused by dissemination of protoscoleces after accidental rupture of fertile cysts and is due to protoscoleces ability to develop into new metacestodes. In the experimental model of secondary cystic echinococcosis mice react against protoscoleces producing inefficient immune responses, allowing parasites to develop into cysts. Although the chronic phase of infection has been analyzed in depth, early immune responses at the site of infection establishment, e.g., peritoneal cavity, have not been well studied. Because during early stages of infection parasites are thought to be more susceptible to immune attack, this work focused on the study of cellular and molecular events triggered early in the peritoneal cavity of infected mice.Principal FindingsData obtained showed disparate behaviors among subpopulations within the peritoneal lymphoid compartment. Regarding B cells, there is an active molecular process of plasma cell differentiation accompanied by significant local production of specific IgM and IgG2b antibodies. In addition, peritoneal NK cells showed a rapid increase with a significant percentage of activated cells. Peritoneal T cells showed a substantial increase, with predominance in CD4+ T lymphocytes. There was also a local increase in Treg cells. Finally, cytokine response showed local biphasic kinetics: an early predominant induction of Th1-type cytokines (IFN-γ, IL-2 and IL-15), followed by a shift toward a Th2-type profile (IL-4, IL-5, IL-6, IL-10 and IL-13).ConclusionsResults reported here open new ways to investigate the involvement of immune effectors players in E. granulosus establishment, and also in the sequential promotion of Th1- toward Th2-type responses in experimental secondary cystic echinococcosis. These data would be relevant for designing rational therapies based on stimulation of effective responses and blockade of evasion mechanisms.

show abstract

Section: Discussionmentioning

confidence: 66%

Early Peritoneal Immune Response during Echinococcus granulosus Establishment Displays a Biphasic Behavior

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Polyclonal B cell activation (86,87) and parasite Fc-like receptors (88)(89)(90)(91) are thought to be partially responsible for anti-parasite ineffective humoral responses. In diverse infection models of strong polyclonal B cells responses, the most common isotypic distribution pattern is characterized by an increase in IgG2 subclasses (87,(92)(93)(94).…”

Section: Discussionmentioning

confidence: 99%

Echinococcus granulosus glycoconjugates induce peritoneal B cell differentiation into antibody‐secreting cells and cytokine production

Mourglia‐Ettlin

Vesely

Fraga

et al. 2011

Parasite Immunology

View full text Add to dashboard Cite

Helminth parasite infections are associated with predominant Th2-type cytokine responses, and parasite glycoconjugates have been recognized as partially responsible for such immune bias. It has been proved that Echinococcus granulosus evokes a Th2-type cytokine pattern characterized by a high production of IL-4, IL-5, IL-6 and IL-10, and no or mild IFN-γ levels in animal models and in patients with cystic echinococcosis, respectively. Here, we show that E4(+) (a glycoconjugate-enriched fraction from E. granulosus protoscolex) stimulated the secretion of a high concentration of IL-6, followed by IL-10 and TNF-α by normal peritoneal B cells. We determined that E4(+) bound to the surface of peritoneal B cells and induced their activation and, also, triggered the differentiation of peritoneal B cells into IgM-, IgG2b- and IgG3-secreting cells in a T-independent way. Interestingly, the IgM released by E4(+) -stimulated peritoneal B cells from normal mice recognized protoscolex antigens. Results showed that, after the encounter with antigens from E. granulosus protoscolex, peritoneal B cells are a source of Th2-type cytokines and polyclonal antibodies, some of which recognize parasite antigens, suggesting that peritoneal B cells can condition the outcome of the infection.

show abstract

“…Proteases secreted by T. cruzi trypomastigotes can enzymolyze the Fc fragment of normal IgG, suggesting that the FcRs inhibit the cytotoxic effects of the classical pathway 38 39 . Furthermore, a fraction (PSA-Fc + ) isolated from the suckers and tegument of E. granulosus protoscoleces was described to possess proteolytic activity specific for human IgG1 and IgG3 through Fc binding 40 . In the present study, we identified six cathepsins in S. japonicum that exhibited an affinity for human Igs.…”

Section: Discussionmentioning

confidence: 99%

Non-immune immunoglobulins shield Schistosoma japonicum from host immunorecognition

Hou

Piao

et al. 2015

Sci Rep

View full text Add to dashboard Cite

Schistosomiasis is a major human parasitic disease with a global impact. Schistosoma japonicum, the most difficult to control, can survive within host veins for decades. Mechanisms of immune evasion by the parasite, including antigenic variation and surface masking, have been implicated but not well defined. In this study, we defined the immunoglobulin-binding proteomes of S. japonicum using human IgG, IgM, and IgE as the molecular bait for affinity purification, followed by protein identification by liquid chromatography with tandem mass spectrometry (LC-MS/MS). Several proteins situated at the tegument of S. japonicum were able to nonselectively bind to the Fc domain of host immunoglobulins, indicating a mechanism for the avoidance of host immune attachment and recognition. The profile of the immunoglobulin-binding proteomes provides further clues for immune evasion mechanisms adopted by S. japonicum.

show abstract

Fc‐binding molecules specific for human IgG1 and IgG3 are present in Echinococcus granulosus protoscoleces

Cited by 10 publications

References 23 publications

Early Peritoneal Immune Response during Echinococcus granulosus Establishment Displays a Biphasic Behavior

Early Peritoneal Immune Response during Echinococcus granulosus Establishment Displays a Biphasic Behavior

Echinococcus granulosus glycoconjugates induce peritoneal B cell differentiation into antibody‐secreting cells and cytokine production

Non-immune immunoglobulins shield Schistosoma japonicum from host immunorecognition

Contact Info

Product

Resources

About