FBXW24 controls female meiotic prophase progression by regulating SYCP3 ubiquitination

Wang, Yang; Gao, Weiyi; Wang, Lili; Wang, Ruo-Lei; Yang, Zhiyun; Luo, Fu‐Qiang; He, Yu-Hao; Wang, Zi‐Bin; Wang, Fu‐Qiang; Sun, Qing‐Yuan; Li, Jing; Zhang, Dong

doi:10.1002/ctm2.891

Cited by 5 publications

(3 citation statements)

References 44 publications

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“…Additionally, it has been demonstrated that tdrd12 exhibits predominant expression in the acrosome, and plays a role in acrosome development [43]. SYCP3 is a component of the synaptic complex that governs homologous recombination during meiosis, and it is also involved in meiotic regulation [44]. The cytoplasmic ribonucleoprotein granule TDRD5 is involved in RNA processing during spermatogenesis [45].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome profiling reveals that differences in regulatory patterns of gonad development between male and female largemouth bass (Micropterus salmoides) contribute to dimorphic weight gain

Wang,

Lu,

Hua

et al. 2024

Preprint

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Background Largemouth bass (Micropterus salmoides) is a sexually dimorphic oviparous species with a significant disparity in gonad size between males and females. Analyzing the mechanism of gonad development is crucial for studying individual growth and development in largemouth bass. Methods In this study, histological analyses, transcriptome sequencing, and fluorescent in situ hybridization analyses were employed to examine the disparities in gonad development between male and female largemouth bass. Results The results indicated a significantly higher growth rate of females than of males, with the ovaries exhibiting greater fullness than the testes. The weight of the mature ovaries was four to five times that of the testes. High transcript levels of acsl5, cidec, lrp13, and elovl1a in the ovary were related to the accumulation of lipid droplets and yolk particles in oocytes. Among the genes with high transcript levels in the ovary, gdf9, ccnb1, ldlrap1a, srd5a1, hsd17b12a, and lfng encoded proteins involved in the regulation of follicle development, oocyte proliferation and differentiation, and steroid hormone synthesis to promote ovarian development. Some of the genes showing high transcript levels in the testes (tekt1, mns1, lrguk, spag6, spef2, and nme5) were implicated in flagella formation, and tdrd5 and star were involved in sperm development. Some of the identified sex-determining genes also exerted significant influences on gonad development. The gene dmrt1, which was specific expressed in spermatogonia, likely plays a crucial role in cell proliferation, whereas btg4, which was specific expressed in the cytoplasm of female oocytes, may participate in regulating meiosis and facilitating oocyte cytoplasm maturation. Conclusions There was an enhancement in the biosynthesis of lipid droplets and yolk granules in oocytes, increasing oocytes cell growth and proliferation. As well as a significant number of genes concentrated on the formation of sperm flagella and other structures. Differences in gene expression led to an increase in the difference in volume between spermatogonia and oocytes during their respective maturation processes, resulting in a significant disparity in both size and weight between the testes and the ovaries. Our findings offer valuable insights into the mechanisms underlying gonad development of largemouth bass.

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Section: Discussionmentioning

confidence: 99%

Transcriptome profiling reveals that differences in regulatory patterns of gonad development between male and female largemouth bass (Micropterus salmoides) contribute to dimorphic weight gain

Wang,

Lu,

Hua

et al. 2024

Preprint

View full text Add to dashboard Cite

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“…1A), thereby promoting their degradation by the proteasome (16). Depleting SCF subunits during meiosis leads to severe synapsis defects in fungi, plants, and animals (17)(18)(19)(20)(21)(22)(23)(24)(25), indicating a pervasive role of SCF in regulating SC assembly. In C. elegans, the SCF complex containing an F-box protein progression of meiosis (PROM)-1 (SCF PROM-1 ) targets a Protein phosphatase 2C (PP2C) phosphatase Protein phosphatase 1D (PPM-1.D) for degradation during the transition from mitosis to meiosis, leading to activation of Checkpoint kinase 2 (CHK-2) kinase and initiation of meiotic chromosome dynamics (26)(27)(28).…”

Section: Introductionmentioning

confidence: 99%

Skp1 proteins are structural components of the synaptonemal complex in C. elegans

Blundon,

Cesar,

Bae

et al. 2024

Sci. Adv.

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The synaptonemal complex (SC) is a zipper-like protein assembly that links homologous chromosomes to regulate recombination and segregation during meiosis. The SC has been notoriously refractory to in vitro reconstitution, thus leaving its molecular organization largely unknown. Here, we report a moonlighting function of two paralogous S-phase kinase-associated protein 1 (Skp1)–related proteins (SKR-1 and SKR-2), well-known adaptors of the Skp1–Cul1–F-box (SCF) ubiquitin ligase, as the key missing components of the SC in Caenorhabditis elegans . SKR proteins repurpose their SCF-forming interfaces to dimerize and interact with meiosis-specific SC proteins, thereby driving synapsis independent of SCF activity. SKR-1 enables the formation of the long-sought-after soluble complex with previously identified SC proteins in vitro, which we propose it to represent a complete SC building block. Our findings demonstrate how a conserved cell cycle regulator has been co-opted to interact with rapidly evolving meiotic proteins to construct the SC and provide a foundation for understanding its structure and assembly mechanisms.

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“…26 Several F-box proteins have been demonstrated to play crucial roles in homolog pairing, synapsis/desynapsis, and meiotic recombination across diverse species, and their substrates have started to emerge. 11,12,[27][28][29][30][31][32] However, the list is nowhere near complete, and it is unknown how the SCF complex recognizes a variety of substrates to regulate meiotic chromosome dynamics.…”

Section: Introductionmentioning

confidence: 99%

Skp1 proteins are structural components of the synaptonemal complex inC. elegans

Blundon

César

Bae

et al. 2023

Preprint

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SummaryThe synaptonemal complex (SC) is a hallmark of meiotic prophase that plays a crucial role in regulating crossovers between homologous chromosomes. Here, we demonstrate that two Skp1-related proteins inC. elegans, SKR-1 and SKR-2, serve as structural components of the SC, independent of their canonical functions within the Skp1-Cul1-F-box (SCF) ubiquitin ligase complex. SKR-1 and SKR-2 localize to the central region of the SC, and synapsis requires their dimerization through a hydrophobic interface that overlaps with the binding sites for CUL-1 and F-box proteins. Usingin vitroreconstitution andin vivoanalysis of mutant proteins, we show that SKR proteins interact with the other SC proteins using their C-terminal helices to form a soluble complex, which likely represents a basic building block for SC assembly. Our findings demonstrate how conserved Skp1 proteins are repurposed as part of the SC and may provide insight into how synapsis is coupled to cell cycle progression.

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FBXW24 controls female meiotic prophase progression by regulating SYCP3 ubiquitination

Abstract: Correct female meiotic prophase is critical for stable genetic passage. Wang et al. identified a novel E3 ubiquitin ligase, FBXW24, which regulated female meiotic prophase progression from pachytene to metaphase II by promoting SYCP3 degradation and double-strand break repair

Cited by 5 publications

References 44 publications

Transcriptome profiling reveals that differences in regulatory patterns of gonad development between male and female largemouth bass (Micropterus salmoides) contribute to dimorphic weight gain

Transcriptome profiling reveals that differences in regulatory patterns of gonad development between male and female largemouth bass (Micropterus salmoides) contribute to dimorphic weight gain

Skp1 proteins are structural components of the synaptonemal complex in C. elegans

Skp1 proteins are structural components of the synaptonemal complex inC. elegans

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