Favourable pregnancy results with insemination of in vitro matured oocytes from unstimulated patients

Söderström‐Anttila, Viveca; Mäkinen, Sirpa; Tuuri, Timo; Suikkari, Anne‐Maria

doi:10.1093/humrep/deh768

Cited by 165 publications

(133 citation statements)

References 26 publications

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“…IVM reported success rates vary considerably among services [1,2,4,8,11,17,18,19,21]. It is acknowledged that the methodology for IVM of oocytes collected from unstimulated ovaries and embryo development is still not ideal [12].…”

Section: Introductionmentioning

confidence: 99%

Fertilization outcome, embryo development and birth after unstimulated IVM

Bös-Mikich

Ferreira²,

Höher³

et al. 2010

J Assist Reprod Genet

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Background/Purposes A common observation in oocyte in vitro maturation (IVM) cycles is poor embryo quality. However, no study was dedicated to assess zygote and early cleavage embryo quality in IVM cycles. The objective of this study is to analyze fertilization outcome, embryo development and the resulting pregnancy and births in unstimulated IVM cycles. Methods IVM oocytes were collected 36 h post hCG and matured in vitro for 28-30 h. All oocytes were inseminated by ICSI. Resulting zygotes and embryos were assessed on day-1, 2 and 3, when transfers were made. Results The overall oocyte maturation and fertilization rates were 63% and 62%, respectively. Abnormal fertilization rate was 1.7%. Ninety five and 14.6% of the 2Pn zygotes reached the 2-cell and 8 -cell stage at day-2 and day-3, respectively. Embryo quality assessment on day-3 at transfer revealed that only 9% of the embryos were of very good quality. Most embryos showed developmental delay. An average of 3.29 embryos were transferred per patient resulting in implantation and clinical gestation rates of 16% and 32%, respectively. Overall 14 healthy babies were born and there is one ongoing pregnancy. ConclusionResults show a significant rate of abnormal fertilization and poor embryo quality after IVM, which is reflected in a higher than average number of embryos being transferred. However, pregnancy, implantation and birth rates are reasonably high and allow us to consider IVM a valuable approach for the treatment of infertility in PCO or PCOS patients.

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Section: Introductionmentioning

confidence: 99%

Fertilization outcome, embryo development and birth after unstimulated IVM

Bös-Mikich

Ferreira²,

Höher³

et al. 2010

J Assist Reprod Genet

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“…Likewise, in normally ovulating women the single most important factor that may affect the IVM outcome is the number of available oocytes which, if inadequately small, can deny the possibility to select embryos from a larger cohort [45,46].…”

Section: Discussionmentioning

confidence: 99%

Anti-mullerian hormone as a predictive marker for the selection of women for oocyte in vitro maturation treatment

Fadini

Comi

Renzini

et al. 2011

J Assist Reprod Genet

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Purpose In oocyte in-vitro maturation (IVM) treatments, the chances to achieve a pregnancy are critically dependent on the retrieval of a suitable number of oocytes. In this study, we assessed the ability of circulating levels of anti-mullerian hormone (AMH) to identify normo-ovulatory women suitable for IVM treatment on the basis of the number of retrieved oocytes. Method Serum AMH was quantified in normo-ovulatory women younger than 39 years undergoing IVM treatment. After immature oocyte retrieval and IVM, maximum 3 mature oocytes were used for treatment and all resulting embryos were transferred, as established by law. From 177 cycles, 991 oocytes were recovered. Following IVM, 484 mature oocytes were obtained (50.1%). Results The overall pregnancy rate per embryo transfer was 16.6% (25/151) and the implantation rate was 10.9% (30/ 278). Linear regression and receiver operating characteristic (ROC) analyses were applied to identify independent variables and quantify a cut-off AMH value able to identify patients suitable for IVM treatment. An AMH value of 1.28 ng/ml was identified as a threshold for the prediction of the retrieval of at least 5 oocytes, with a sensitivity of 93.4% and a specificity of 33.8%. Positive and negative predictive values were 67.6% and 75.0%, respectively. Conclusions AMH can be adopted to identify women candidate for an IVM treatment from whom a suitable number of oocytes may be retrieved. This is of crucial significance during a non-stimulated cycle, in order to prevent an insufficient oocyte collection and rescue the treatment by implementing a conventional controlled ovarian stimulation.

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“…In brief, it seems that IVM is less effective than conventional IVF (8). In IVF, up to 59% of delivery rates have been achieved after fresh and subsequent frozen embryo transfers (16), whereas in IVM, delivery rates of 13% to 15% have been achieved (17,18). In some studies, the lower rates of pregnancy were attributed to lower quality of endometrium for implantation (19), which may be critical in assisted reproductive technology results (20).…”

Section: Discussionmentioning

confidence: 99%

Comparison of In-Vitro Maturation and In-Vitro Fertilization in Infertile Females with Polycystic Ovary Syndrome

Jafarabadi

Ramezanzadeh

Salimi

et al. 2017

J Obstet Gynecol Cancer Res

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Background: Both in-vitro maturation and in-vitro fertilization have been used successfully to treat females with polycystic ovarian syndrome, who plan to have child. This study compared outcome of these two approaches to fertilize females with polycystic ovary side effects. Methods: This prospective study was conducted at Vali-Asr reproductive health research center and included all females with polycystic ovarian syndrome, who underwent in-vitro maturation or in-vitro fertilization from January 2005 to January 2008. Measurements included demographic data, total cost (including drug and laboratory procedures), outcome (chemical and clinical pregnancy), and systemic complication (ovarian hyper-stimulation syndrome), obtained from the patients' clinical files. Patients were informed about the procedures, side effects and goals, and the signed consent form for surgical approaches and use of their data in medical research. Finally, these variables were compared between the two groups. Results: The in-vitro maturation and in-vitro fertilization groups included 20 and 22 patients, respectively. The range of body mass

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Favourable pregnancy results with insemination of in vitro matured oocytes from unstimulated patients

Abstract: Insemination of IVM oocytes functions well, resulting in comparable pregnancy rates per IOC between IVM-IVF and IVM-ICSI. Good pregnancy results can be achieved both in patients with regular cycles and with PCO(S) by transferring only one or two embryos at a time.

Cited by 165 publications

References 26 publications

Fertilization outcome, embryo development and birth after unstimulated IVM

Fertilization outcome, embryo development and birth after unstimulated IVM

Anti-mullerian hormone as a predictive marker for the selection of women for oocyte in vitro maturation treatment

Comparison of In-Vitro Maturation and In-Vitro Fertilization in Infertile Females with Polycystic Ovary Syndrome

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