Favoring the birth of female puppies after artificial insemination using chilled semen diluted with powdered coconut water (ACP-106c)

Uchoa, Daniel Couto; Silva, Ticiana Franco Pereira da; Cardoso, Janaina de Fátima Saraiva; Filho, Antônio Cavalcante Mota; Jucá, Ricardo Parente; Silva, Alexandre Rodrigues; Silva, Lúcia Daniel Machado da

doi:10.1016/j.theriogenology.2011.11.029

Cited by 10 publications

(8 citation statements)

References 11 publications

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“…Although our team has shown that semen diluted in ACP-106c and cooled prior to insemination led to increased birth of female pups in the French Bulldog (Uchoa et al 2012), the same effect was not observed in the present study, probably due to the too small number of pups to show any effect of gender.…”

Section: Discussioncontrasting

confidence: 85%

“…) and for artificial insemination (AI) with cooled semen in dogs (Uchoa et al. ); however, there are no reports of normal pregnancy when using ACP‐106c with frozen/thawed canine semen.…”

Section: Introductionmentioning

confidence: 99%

“…A powdered coconut water-based extender ACP-106c (ACP ® ; ACP Servic¸os Tecnolo´gicos Ltda, ACP Biotecnologia, Fortaleza, Ceara´, Brazil) has been developed in recent years. This medium has been used for semen freezing (Cardoso et al 2007) and for artificial insemination (AI) with cooled semen in dogs (Uchoa et al 2012); however, there are no reports of normal pregnancy when using ACP-106c with frozen/thawed canine semen.…”

Section: Introductionmentioning

confidence: 99%

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Intravaginal Artificial Insemination in Bitches Using Frozen/Thawed Semen after Dilution in Powdered Coconut Water (ACP‐106c)

Uchoa

Silva

Filho

et al. 2012

Reprod Domestic Animals

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ContentsThe aim of this study was to evaluate powdered coconut water extender (ACP-106c; ACP Servic¸os Tecnolo´gicos Ltda, ACP Biotecnologia, Fortaleza, Ceara´, Brazil) as a diluent for freezing dog semen and the fertility after vaginal insemination of semen frozen therein. Ten ejaculates were collected from five dogs, evaluated fresh, diluted in ACP-106c, 10% egg yolk and 6% glycerol, cooled and frozen. In the first phase of the study, straws with frozen semen were thawed and immediately subjected to the same analysis as the fresh semen and, in addition, to Computer-Assisted Semen Analysis (CASA). In phase 2, 10 bitches that had been subjected to natural breeding during a preceding oestrous cycle were vaginally inseminated with thawed semen that had been re-diluted in ACP-106c. After thawing, a mean of 77% sperm motility was obtained through subjective analysis and 77.3% through CASA. Following artificial insemination, a 60% pregnancy rate was observed, resulting in a 50% parturition rate and a mean litter size of 3.4 (SEM 0.6), with 47.1% males and 52.9% females. ACP-106c can be successfully used for freezing canine semen, and vaginal deposition of such semen yields similar pregnancy rates to those reported in other studies.

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Section: Discussioncontrasting

confidence: 85%

“…) and for artificial insemination (AI) with cooled semen in dogs (Uchoa et al. ); however, there are no reports of normal pregnancy when using ACP‐106c with frozen/thawed canine semen.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Intravaginal Artificial Insemination in Bitches Using Frozen/Thawed Semen after Dilution in Powdered Coconut Water (ACP‐106c)

Uchoa

Silva

Filho

et al. 2012

Reprod Domestic Animals

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“…Os parâmetros mensurados na avaliação computadorizada das amostras foram: motilidade total, motilidade progressiva, VAP, VCL, VSL, STR e LIN (Mota Filho et al 2011, Uchoa et al 2012.…”

Section: Methodsunclassified

“…O Tris é um diluidor que já se mostrou eficiente para conservação de espermatozoide epididimário canino (Hewit et al 2001, Ponglowhapan et al 2006. Por outro lado, o diluidor ACP--106c já demonstrou ser igualmente eficaz na conservação de sêmen canino refrigerado (Uchoa et al 2012) sendo também utilizado para a conservação de espermatozoides do epidídimo de cutias . No entanto, esse diluidor à base de água de coco em pó ainda não foi testado para recuperação e conservação de espermatozoides epididimários de cão, podendo ser um diluidor alternativo e interessante para conservação de espermatozoides epididimários nessa espécie, como o foi para cutias.…”

Section: Introductionunclassified

Refrigeração do epidídimo canino a 4ºC e recuperação dos espermatozoides epididimários utilizando ACP-106c

et al. 2013

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. 2013. Objetivou-se avaliar a qualidade dos espermatozoides recuperados da cauda do epidídimo após a refrigeração do complexo testículo-epidídimo (CTE) de cães usando o diluidor ACP-106c. Foram utilizados 60 cães machos adultos, com peso de 10-20 kg.Após a eutanásia, removeu-se o CTE que foi imerso em solução fisiológica 0,9% e transportado em caixa térmica ao laboratório a 30°C. Para a refrigeração e recuperação dos espermatozoides epididimários, os 60 pares do CTE foram divididos em 4 grupos, de acordo com o tempo de refrigeração do CTE e posterior recuperação espermática: G0h, G6h, G12h e G18h, em que cada par do CTE permaneceu por zero, seis, doze ou dezoito horas a 4°C, respectivamente. A recuperação dos espermatozoides da cauda do epidídimo foi realizada pela técnica de flutuação utilizando-se o diluidor ACP-106c ou Tris. Para cada epidídimo foi adicionado 1,0 mL de um dos dois diluidores, pré-aquecidos a 37°C por 5 minutos. Em seguida foram centrifugados a 800g/5 minutos para remoção dos resíduos celulares. Avaliou-se The study aimed to assess the quality of sperm recovered from the epididymal cauda after cooling the complex testis-epididymis (CTE) of dogs using ACP-106c extender. Sixty adult male dogs, weighing 10-20 kg were used. After euthanasia, CTE was removed and it was immersed in 0.9% saline and transported to the laboratory in cooler at 30°C. For cooling and recovery of epididymal spermatozoa, the 60 pairs of CTE were divided into four groups, according to the refrigeration time of the CTE and subsequent sperm recovery: G0h, G6h, G12h and G18h, wherein each pair of CTE remained zero, six, 12 or 18 hours at 4°C respectively. The recovery of sperm epididymal tail was conducted by flotation technique using ACP-106c or Tris extender. For each epididymis, it was added 1.0 mL of either extenders, preheated to 37°C for 5 minutes. They were then centrifuged at 800g/5 minutes to remove the cell debris. Morphology, functionality and total and progressive motility, and parameters obtained by CASA were evaluated. Data were analyzed by ANOVA followed by Turkey test (P <0.05). In all parameters assessed, there was no difference between the extenders used (P>0.05). The values of total motility groups G0h, G6H, G12H, and G18h for ACP-106c were 84.4±7.7, 81.6±11.6, 88.3±6.5 and 69.5±16.9 respectively, and for Tris 85.2±8.7, 77.4±14.3, 79.0±17.8 and 65.4±17.9 respectively. A decrease in sperm quality was observed after 18 hours of cooling in both extenders. Thus it can be concluded that the ACP-106c may be used to recover the epididymal spermatozoa chilled and may be viable for up to 12h cooling.INDEX TERMS: Refrigeration, epididymis, dogs.

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Seminal coagulation and sperm quality in different social contexts in captive tufted capuchin monkeys (Sapajus apella)

Lima

Leão

Oliveira

et al. 2017

American J Primatol

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In the present study, we aimed to assess the influence of different social contexts on the seminal coagulation and sperm quality in captive tufted capuchin monkeys. For this, males were housed either individually, in mixed-sex groups (with females), or in male-only groups. Monkeys were housed in cages and each cage type (i.e., individual or group cage) was placed in a different room. Forty-one males were subjected to semen collection by rectal electroejaculation. The degree of seminal coagulation was determined on a scale of I-IV. Seminal volume, sperm concentration, sperm motility, vigor, and plasma membrane integrity were evaluated for all ejaculate samples. All ejaculates collected showed degrees of coagulation between II and IV, where the majority presented coagulation degree IV, when collected from animals housed in groups. No statistical differences among percentages of coagula degree when samples were collected from males housed individually. Animals housed in group cages (male-only groups and mixed-sex groups) showed a significantly higher percentage of ejaculates at degree IV than males housed individually. Seminal volume was not affected by the coagula degree but by the housing system, where animals housed individually showed the highest volume (543 μl) when compared with those animals from male (273 μl) and mixed-sex (318 μl) groups. No differences were observed in semen volume when comparing male-only groups with mixed-sex groups. Sperm motility was affected by both housing system and coagula degree. Samples with coagula degree IV from animals housed individually showed the highest (72%) sperm motility percentages. Sperm plasma membrane integrity was lower when samples were presenting coagula degree II + III and collected from male- (17%) or mixed-sex (23%) groups. However, this housing system effect was not observed when sperm was obtained from coagula degree IV semen. Sperm vigor was neither affect by housing system or coagula degree.

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Favoring the birth of female puppies after artificial insemination using chilled semen diluted with powdered coconut water (ACP-106c)

Cited by 10 publications

References 11 publications

Intravaginal Artificial Insemination in Bitches Using Frozen/Thawed Semen after Dilution in Powdered Coconut Water (ACP‐106c)

Intravaginal Artificial Insemination in Bitches Using Frozen/Thawed Semen after Dilution in Powdered Coconut Water (ACP‐106c)

Refrigeração do epidídimo canino a 4ºC e recuperação dos espermatozoides epididimários utilizando ACP-106c

Seminal coagulation and sperm quality in different social contexts in captive tufted capuchin monkeys (Sapajus apella)

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