Favorable Periodontal Healing of 1‐Wall Infrabony Defects After Application of Calcium Phosphate Cement Wall Alone or in Combination With Enamel Matrix Derivative: A Pilot Study With Canine Mandibles

Shirakata, Yoshinori; Yoshimoto, Takehiko; Goto, Hisanori; Yonamine, Yutaka; Kadomatsu, Hideshi; Miyamoto, Mari; Nakamura, Toshiaki; Hayashi, Chie; Izumi, Yuichi

doi:10.1902/jop.2007.060353

Cited by 26 publications

(36 citation statements)

References 47 publications

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“…The histological and histomorphometrical findings indicate that the reparative type of healing, as observed in the empty group in our experiment, is consistent with numerous published reports [1, 3, 13, 24]. Our experiment showed that even three-wall periodontal defects did not regenerate spontaneously.…”

Section: Discussionsupporting

confidence: 93%

“…Also in a dog study, empty defects showed only a reparative type of healing while EMD-treated defects demonstrated varying amounts of new cementum but hardly any regenerated bone volume [13]. Adding CaP, however, with or without EMD showed significant more cementum and bone regeneration.…”

Section: Discussionmentioning

confidence: 99%

“…Adding CaP, however, with or without EMD showed significant more cementum and bone regeneration. In this study, the importance of soft tissue support was emphasized while avoiding immediate contact between the biomaterial and the root [13]. Follow-up investigations have to be confirmed whether CaP cement only serves as wound stabilizer or if its osteoconductive properties are the determining factor.…”

Section: Discussionmentioning

confidence: 99%

“…PDL formation included new (a) cellular cementum formation in combination of perpendicularly oriented fibers inserted into the cement and bone. CT attachment was regarded as the supracrestal connective tissue adhesion to the root surface without new cementum formation but in presence of perpendicularly orientated of fibers [13, 14]. Subsequently, groups were compared with ANOVA and post-hoc Tukey testing.…”

Section: Methodsmentioning

confidence: 99%

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Regeneration of the periodontium using enamel matrix derivative in combination with an injectable bone cement

et al. 2012

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ObjectivesEnamel matrix derivative (EMD) has proven to enhance periodontal regeneration; however, its effect is mainly restricted to the soft periodontal tissues. Therefore, to stimulate not only the soft tissues, but also the hard tissues, in this study EMD is combined with an injectable calcium phosphate cement (CaP; bone graft material). The aim was to evaluate histologically the healing of a macroporous CaP in combination with EMD.Materials and methodsIntrabony, three-wall periodontal defects (2 × 2 × 1.7 mm) were created mesial of the first upper molar in 15 rats (30 defects). Defects were randomly treated according to one of the three following strategies: EMD, calcium phosphate cement and EMD, or left empty. The animals were killed after 12 weeks, and retrieved samples were processed for histology and histomorphometry.ResultsEmpty defects showed a reparative type of healing without periodontal ligament or bone regeneration. As measured with on a histological grading scale for periodontal regeneration, the experimental groups (EMD and CaP/EMD) scored equally, both threefold higher compared with empty defects. However, most bone formation was measured in the CaP/EMD group; addition of CAP to EMD significantly enhanced bone formation with 50 % compared with EMD alone.ConclusionsWithin the limits of this animal study, the adjunctive use of EMD in combination with an injectable cement, although it did not affect epithelial downgrowth, appeared to be a promising treatment modality for regeneration of bone and ligament tissues in the periodontium.Clinical relevanceThe adjunctive use of EMD in combination with an injectable cement appears to be a promising treatment modality for regeneration of the bone and ligament tissues in the periodontium.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Regeneration of the periodontium using enamel matrix derivative in combination with an injectable bone cement

et al. 2012

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“…Defect creation : Periodontal defects can either be created surgically (13, 30, 31), involve ligatures around teeth (32, 33), or develop naturally (34, 35). For surgical creation, defects can be made to the same size or shape to compare test groups and controls.…”

Section: Methodsmentioning

confidence: 99%

Preclinical Methods for the Evaluation of Periodontal Regeneration In Vivo

Seol

Pellegrini

Franco

et al. 2010

Methods in Molecular Biology

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For the determination of key factors or devices that promote periodontal regeneration, preclinical investigations using in vivo animal models are critical for evaluating the biological responses before human clinical trial testing. In this chapter, we provide an overview on the commonly used preclinical animals for the study of reconstructive procedures to promote bone and soft tissue repair of tooth-supporting periodontal defects. Steps are provided on the animal management for evaluation of outcome measures using descriptive histology, histomorphometry, three-dimensional imaging, and safety assessments. The use of these key measures of periodontal regeneration should aid investigators in the selection of appropriate surrogate endpoints to be utilized in the clinical arena, which are not practical or ethical in humans. These methods will prepare investigators and assist them in identifying endpoints that can then be adapted to human clinical trial planning.

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Periodontal regeneration using an injectable bone cement combined with BMP-2 or FGF-2

Oortgiesen

Walboomers

Bronckers

et al. 2012

J Tissue Eng Regen Med

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Periodontitis is a frequently diagnosed oral disease characterized by bone resorption and soft tissue loss around teeth. Unfortunately, currently available therapies only slow or arrest progress of the disease. Ideally, treatment of periodontal defects should be focused on complete regeneration of the lost tissues [(bone and periodontal ligament (PDL)]. As a result, this study used intrabony defects to evaluate the regenerative potential of an injectable macroporous calcium phosphate cement (CaP) in combination with bone morphogenetic protein-2 (BMP-2) or fibroblast growth factor-2 (FGF-2). After creating 30 periodontal defects in 15 Wistar rats, three treatment strategies were conducted: application of CaP only, CaP + BMP-2 and CaP + FGF-2. Animals were euthanized after 12 weeks and processed for histology and histomorphometry. Using CaP alone resulted in limited effects on PDL and bone healing. CaP + BMP-2 showed a good response for bone healing; a significant 2.4 fold increase in bone healing score was observed compared to CaP. However, for PDL healing, CaP + BMP-2 treatment showed no difference compared to the CaP group. The best results were observed with the combined treatment of CaP + FGF-2, which showed a significant 3.3 fold increase in PDL healing score compared to CaP + BMP-2 and a significant 2.6 fold increase compared to CaP. For bone healing, CaP + FGF-2 showed a significant 1.9 fold increase compared to CaP but no significant difference was noted compared to the CaP + BMP-2 group. The combination of a topical application of FGF-2 and an injectable CaP seems to be a promising treatment modality for periodontal regeneration.

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Favorable Periodontal Healing of 1‐Wall Infrabony Defects After Application of Calcium Phosphate Cement Wall Alone or in Combination With Enamel Matrix Derivative: A Pilot Study With Canine Mandibles

Abstract: CPC walls with and without EMD promoted regeneration of alveolar bone and cementum in 1-wall infrabony defects. Space and stable wound healing are believed to be crucial for periodontal regeneration in non-contained infrabony defects.

Cited by 26 publications

References 47 publications

Regeneration of the periodontium using enamel matrix derivative in combination with an injectable bone cement

Regeneration of the periodontium using enamel matrix derivative in combination with an injectable bone cement

Preclinical Methods for the Evaluation of Periodontal Regeneration In Vivo

Periodontal regeneration using an injectable bone cement combined with BMP-2 or FGF-2

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