Late Quaternary faunal remains from three underwater settings in Cold Lake, Alberta and Saskatchewan, Canada, include at least 13 vertebrate taxa consistent with assemblages that postdate the Last Glacial Maximum (LGM). Seven new radiocarbon dates range from 10 350 ± 40 to 161 ± 23 years BP and provide insight into the post-LGM biotic history of east-central Alberta and west-central Saskatchewan. The presence of an essentially modern large mammal biota is suggested for the mid-Holocene, and possibly earlier, if the absence of extinct or extirpated taxa in association with Late Pleistocene Bison at the Alberta–Saskatchewan site is meaningful. Taphonomically, some of the remains suggest deposition in open environments during the Holocene, possibly when lake levels were lower. The recovery of late Quaternary faunal remains from a present-day lacustrine setting is novel, and suggests that similar records may occur in other lakes in western Canada, including those in areas with scarce Quaternary vertebrate records.