Fauna helmintológica de bovinos da região de Jaboticabal, Estado de São Paulo, Brasil

Borges, Fernando de Almeida; Silveira, Daniela Miyasaka da; Graminha, Érika Barbosa Neves; Castagnolli, Karina Carrão; Soares, Vando Edésio; Nascimento, Adjair Antônio do; Costa, Alvimar José da

doi:10.5433/1679-0359.2001v22n1p49

Cited by 9 publications

(9 citation statements)

References 4 publications

(5 reference statements)

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“…These results reveal that H. contortus is probably the major nematode species infecting the ruminants tested. This finding is compatible with the helminthic fauna described by Borges et al (2001), Veríssimo et al (2012) and Brasil et al (2012). Since only a fraction of the eggs sampled were identified by means of sequencing (n = 30), it is also not surprising that H. contortus appears to be a major species, given its high prolificacy and consequently its greater relative abundance of eggs, in comparison with other helminths.…”

Section: Resultssupporting

confidence: 71%

Frequency of benzimidazole resistance inHaemonchus contortus populations isolated from buffalo, goat and sheep herds

Nunes

Santos

Bastianetto

et al. 2013

Rev. Bras. Parasitol. Vet.

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Anthelmintic resistance is an increasing problem that threatens livestock production worldwide. Understanding of the genetic basis of benzimidazole resistance recently allowed the development of promising molecular diagnostic tools. In this study, isolates of Haemonchus contortus obtained from goats, sheep and buffaloes raised in Brazil were screened for presence of the polymorphism Phe200Tyr in the β-tubulin 1 gene, which confers resistance to benzimidazole. The allelic frequency of the mutation conferring resistance ranged from 7% to 43%, and indicated that resistance to benzimidazole could be found in nematodes isolated from all the ruminant species surveyed. Although significant variation in the frequency of the F200Y mutation was observed between different herds or host species, no significant variation could be found in populations isolated from animals within the same herd. These findings suggest that screening of samples from a few animals has the potential to provide information about the benzimidazole resistance status of the entire herd, which would enable a considerable reduction in the costs of diagnosis for the producer. Molecular diagnosis has practical advantages, since it can guide the choice of anthelmintic drug that will be used, before its application in the herd, thus reducing the economic losses driven by anthelmintic resistance.Keywords: Anthelmintic resistance, benzimidazole drugs, molecular diagnosis, domestic ruminants, genetic polymorphisms. ResumoA resistência aos anti-helmínticos é um problema crescente que ameaça a produção pecuária em todo o mundo. A compreensão da base genética da resistência ao benzimidazol permitiu, recentemente, o desenvolvimento de métodos diagnósticos moleculares promissores. Neste estudo, isolados de Haemonchus contortus obtidos a partir de rebanhos de caprinos, ovinos e bubalinos criados no Brasil foram avaliados quanto à presença do polimorfismo F200Y no gene da β-tubulina1, o qual confere resistência ao benzimidazol. A frequência alélica da mutação variou de 7% a 43%, indicando que a resistência ao benzimidazol pode ser encontrada em nematoides isolados a partir de todas as espécies de ruminantes pesquisadas. Embora tenha sido observada variação significativa das frequências de mutação F200Y entre rebanhos/espécies hospedeiros distintos, não foi encontrada variação significativa entre populações isoladas de animais dentro de um mesmo rebanho. Estes achados sugerem que a avaliação de amostras de alguns poucos animais tem o potencial de fornecer informações sobre o nível de resistência ao benzimidazol de todo o rebanho, possibilitando uma redução considerável dos custos de diagnóstico para o produtor. O diagnóstico molecular apresenta vantagens práticas, uma vez que pode guiar a escolha da base anti-helmíntica a ser utilizada antes da sua aplicação no rebanho, reduzindo, portanto, as perdas ocasionadas pela resistência aos fármacos anti-helmínticos.Palavras-chave: Resistência a anti-helmínticos, benzimidazóis, diagnóstico molecular, ruminantes domés...

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Section: Resultssupporting

confidence: 71%

Frequency of benzimidazole resistance inHaemonchus contortus populations isolated from buffalo, goat and sheep herds

Nunes

Santos

Bastianetto

et al. 2013

Rev. Bras. Parasitol. Vet.

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“…The lung parasite identified in our study was D. viviparus. These parasites were also the most prevalent observed in previous reports from different parts of Brazil: São Paulo, Rio de Janeiro, and Minas Gerais State (Oliveira, 1988;Gonç alves et al, 2000;Borges et al, 2001;Landim et al, 2001;Neto and Fonseca, 2002;Bruhn et al, 2012), as well in other countries with similar climates, characterized by two well-defined periods: a rainy season (summer) and a dry season (winter), such as in Costa Rica (Jiménez et al, 2010), and Vietnam (Holland et al, 2000).…”

Section: Discussionsupporting

confidence: 55%

“…and Ostertagia spp., parasites of the abomasum; Cooperia spp., Trichostrongylus spp., and Strongyloides spp., parasites of the small intestine and Oesophagostomum spp., parasite of the large intestine. Additionally, the lung nematode Dictyocaulus viviparous has been reported in ruminants in São Paulo State (Oliveira, 1988;Gonç alves et al, 2000;Borges et al, 2001;Landim et al, 2001). These species of gastrointestinal nematodes are reported to be present worldwide (Keyyu et al, 2005;Holland et al, 2000;Jiménez et al, 2010), while, D. viviparous is most frequently described in temperate climates (Ploeger., 2002;Wapenaar et al, 2007;Lat-Lat et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Changes in biochemical analytes in calves infected by nematode parasites in field conditions

Cezaro

Tvarijonaviciute

Tecles

et al. 2016

Veterinary Parasitology

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“…In Brazil, the epidemiology of parasitic infections in bovines was assessed by several authors, in diverse states, including in São Paulo (SP) (Oliveira & Matsumoto 1985, Oliveira 1988, Bresciani et al 2001, Borges et al 2001, Landim et al 2001, Minas Gerais (MG) (Furlong et al 1985, Lima 1998, Araujo & Lima 2005, Santos et al 2010 and Rio de Janeiro (RJ) (Duarte et al 1982, Pimentel Neto & Fonseca 2002. Dealing more specifically with gastrointestinal and pulmonary nematodes, in general, the genera and/or species observed were: Cooperia spp., Haemonchus spp., Trichostrongylus spp., Oesophagostomum spp., Bunostomum spp., Agriostomum wryburgi, Capillaria bovis, Strongyloides papillosus, Trichuris discolor, and less commonly Ostertagia spp.…”

Section: Introductionmentioning

confidence: 99%

Gastrointestinal and pulmonary nematodes in calves naturally infected in the cities of Botucatu and Manduri, in the Brazilian state of São Paulo

et al. 2018

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This study aimed to determine the occurrence of gastrointestinal and pulmonary parasites in calves and to evaluate seasonal and age patterns in parasitism. For this, we used 140 clinically healthy crossbreed calves (two to 12 months old) that belonged to two private farms in the municipalities of Botucatu (n=53) and Manduri (n=87), São Paulo state, Brazil. The calves were monitored for 12 months (from September 2014 to August 2015). Fecal samples were collected directly from the rectum every three months. Fecal egg counts were determined using the modified McMaster technique with a sensitivity of 50 eggs per gram of feces (EPG). Coproculture was performed on pooled samples to identify Strongylida infective larvae. First-stage larvae of Dictyocaulus viviparus were extracted by a modified Baermann method. The data showed non-normal distribution (Shapiro-Wilk) and the nonparametric Kruskall-Wallis test was employed to evaluate the EPG data by seasons and age groups. Dunn’s post-test was used for multiple comparisons (P<0.05). The calves from Manduri farm showed significantly higher fecal egg counts (P<0.0001) in the winter when compared to other seasons. At Botucatu farm, young calves (2-3 months old) showed significantly higher EPG than old calves (8-12 months) (P=0.01). The prevalence and overall mean of animals positive for Strongylida type-eggs were 81.1% and 340 in Botucatu, respectively, versus 83.9% and 854 in Manduri, respectively. Furthermore, we found Strongyloides spp., Moniezia spp., and Trichuris spp. eggs and Eimeria spp. oocysts. The prevalent genera in all coprocultures in decreasing order were: Cooperia spp., Haemonchus spp., Oesophagostomum spp., and Trichostrongylus spp. First-stage larvae of Dictyocaulus viviparus were found only in Botucatu farm samples throughout the year, except in spring.

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Fauna helmintológica de bovinos da região de Jaboticabal, Estado de São Paulo, Brasil

Cited by 9 publications

References 4 publications

Frequency of benzimidazole resistance inHaemonchus contortus populations isolated from buffalo, goat and sheep herds

Frequency of benzimidazole resistance inHaemonchus contortus populations isolated from buffalo, goat and sheep herds

Changes in biochemical analytes in calves infected by nematode parasites in field conditions

Gastrointestinal and pulmonary nematodes in calves naturally infected in the cities of Botucatu and Manduri, in the Brazilian state of São Paulo

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