Fault isolation at P/N junction by nanoprober

Liu, Wanyi; Chiang, Chih-Feng; Yen, Chia-Hsiang; Lin, Rung-Jiun; Chang, T. H. P.; Chang, Shang‐Hung; Kuo, Pau-Sheng; Chu, Chih-Hsun

doi:10.1016/j.microrel.2016.07.081

Cited by 1 publication

(1 citation statement)

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“…Twenty-one antibiotic discs (Oxoid, UK) were used, including: 30 μg of amikacin (AK30), 30 μg of amoxicillin/clavulanic acid (AMC30), 10 μg of ampicillin (AM10), 10 μg of bacitracin (B10), 30 μg of cephalothin (KF30), 30 μg of cefuroxime sodium (CXM30), 30 μg of cefotaxime (CTX30), 5 μg of ciprofloxacin (CIP5), 2 μg of clindamycin (CC2), 10 μg of colistin (CL10), 15 μg of erythromycin (E15), 10 μg of gentamicin (GM10), 2 μg of lincomycin (MY2), 30 μg of nalidixic acid (NA30), 30 μg of neomycin (N30), 10 μg of norfloxacin (NOR10), 5 μg of ofloxacin (OFX5), 10U of penicillin G (P10), 10 μg of streptomycin (S10), 23.75/1.25 μg of sulphamethoxazole/trimethoprim (SXT25), and 30 μg of tetracycline (TE30). Among these antibiotics, amoxicillin/clavulanic acid, ampicillin, colistin, erythromycin, gentamicin, lincomycin, nalidixic acid, neomycin, penicillin, streptomycin, sulphamethoxazole, trimethoprim, and tetracycline are approved for use in waterfowl in Taiwan (19). A 0.5 McFarland bacterial suspension in sterile normal saline was inoculated on Mueller Hinton Agar supplemented with 5% sheep blood (Becton Dickinson, USA), and the antibiotic discs were placed on the agar plates, which were then cultured for 24 h at 37ºC with 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

Epidemiology and antibiogram of Riemerella anatipestifer isolated from waterfowl slaughterhouses in Taiwan

Chang

Chen

Wang

et al. 2019

Journal of Veterinary Research

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Introduction: Laryngeal swab samples collected from three waterfowl slaughterhouses in central Taiwan were cultured and suspected isolates of Riemerella anatipestifer were identified by API 20NE and 16S rDNA PCR. Material and Methods: Serum agglutination was used for serotyping, and antimicrobial susceptibility was tested. Results: Seventy-six R. anatipestifer isolates were detected, and the prevalences in the ducks and geese were 12.3% (46/375) and 8.0% (30/375), respectively. The positive isolation rates were 65.6% for all arriving waterfowl, 76.0% for birds in the holding area, 1.6% for defeathered carcasses, but zero for degummed carcasses. A PCR examination detected R. anatipestifer in the slaughtering area frequently. Serotype B was dominant in both duck (34.8%) and goose (46.7%) isolates, but the wide serotype distribution may very well impede vaccination development. All isolates were resistant to colistin, and 79.7% were resistant to more than three common antibiotics. Conclusion: The results proved that most ducks had encountered antibiotic-resistant R. anatipestifer in rearing, which suggests that the bacterium circulates in asymptomatic waterfowl. It is worth noting that most waterfowl farms were found to harbour R. anatipestifer, and contaminated slaughterhouses are a major risk factor in its spread. Effective prevention and containment measures should be established there to interrupt the transmission chain of R. anatipestifer.

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Section: Methodsmentioning

confidence: 99%