The purpose of our study was to develop 3D tumour spheroids of human colon cancer cells and to evaluate the therapeutic potential of NP1, a novel cell penetrating peptide (CPP) developed by our group, to assist in siRNA delivery. NP1 elicited significant cellular uptake of siRNA and promoted great siRNA knockdown efficiency of Bcl-2 and VEGF mRNA in 3D spheroids, induced marked apoptosis after silencing HIF mRNA, and 3D spheroids displayed apoptosis resistance compared to 2D cells. Therefore, 3D spheroids provide an improved model for testing siRNA delivery and NP1 has proven to be a powerful in vitro transfection reagent. [1] X-ray diffraction is the most frequent analytical technique that chemical engineers cite as a keyword. The major research clusters include nanoparticles, composites, adsorption phenomena, catalysis, and, in particular, photocatalysis. It measures lattice constants, average grain size, crystallinity, and phase composition. When radiation impinges upon a solid, coherent scattering of the radiation by periodically spaced atoms produces spot patterns from single crystal samples and ring patterns from polycrystalline samples. The intensities of the diffraction maxima (peaks or lines) and their position correlate to a specific crystal structure. Most analysis errors are due to sample type or instrument operation/preparation. [2] Fault Detection, Supervision, and Safety for Chemical Processes: 2020Youqing Wang, Donghua Zhou This special issue section includes ten articles that provide an up-to-date and comprehensive picture of safety methods and techniques for chemical processes. In particular, these articles focus on the following problems: fault diagnosis, remaining useful life prediction, and quality prediction. In terms of methodology, this special issue section involves deep learning, principal component analysis, the observer-based method, and the filter-based method. The proposed methods have been tested on an industrial E. coli fermentation process, oil-drilling process, penicillin fermentation process, threetank system, large blast furnace, and lithium-ion battery, respectively. These articles cover a range of topics of interest to the control community at large and to the readers of this journal in particular. [3] variable 0 5 10 15 20 25 WMK 1 2 3 4 5 WMK thresholdA process monitoring method based on correlation variable classification and vine copula is proposed. The weighted correlation measure is used to divide the variables into related subspace and weakly correlated subspace. C-vine and D-vine copulas are used to model the joint probability distributions of the two subspaces, respectively. Process faults are detected according to the two joint probability distributions. The monitoring results of a numerical system and the Tennessee Eastman (TE) process have been used to demonstrate the effectiveness and validity of the proposed method. [4]
REFERENCES[1] K.