Fatty aldehyde dehydrogenases in Acinetobacter sp. strain HO1-N: role in hexadecanol metabolism

Singer, Mary E.; Finnerty, W. R.

doi:10.1128/jb.164.3.1011-1016.1985

Cited by 25 publications

(3 citation statements)

References 16 publications

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“…Instead, we hypothesise that the degradation of dodecane in this study was via an alkyl hydroperoxide system. This alternative alkane biodegradation pathway was first postulated by Singer and Finnerty [ 66 ] and observed in Acinetobacter sp. [ 67 , 68 ].…”

Section: Discussionmentioning

confidence: 85%

Bacterial Utilisation of Aliphatic Organics: Is the Dwarf Planet Ceres Habitable?

Jayasinghe

Kennedy

McMinn

2022

Life

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The regolith environment and associated organic material on Ceres is analogous to environments that existed on Earth 3–4 billion years ago. This has implications not only for abiogenesis and the theory of transpermia, but it provides context for developing a framework to contrast the limits of Earth’s biosphere with extraterrestrial environments of interest. In this study, substrate utilisation by the ice-associated bacterium Colwellia hornerae was examined with respect to three aliphatic organic hydrocarbons that may be present on Ceres: dodecane, isobutyronitrile, and dioctyl-sulphide. Following inoculation into a phyllosilicate regolith spiked with a hydrocarbon (1% or 20% organic concentration wt%), cell density, electron transport activity, oxygen consumption, and the production of ATP, NADPH, and protein in C. hornerae was monitored for a period of 32 days. Microbial growth kinetics were correlated with changes in bioavailable carbon, nitrogen, and sulphur. We provide compelling evidence that C. hornerae can survive and grow by utilising isobutyronitrile and, in particular, dodecane. Cellular growth, electron transport activity, and oxygen consumption increased significantly in dodecane at 20 wt% compared to only minor growth at 1 wt%. Importantly, the reduction in total carbon, nitrogen, and sulphur observed at 20 wt% is attributed to biotic, rather than abiotic, processes. This study illustrates that short-term bacterial incubation studies using exotic substrates provide a useful indicator of habitability. We suggest that replicating the regolith environment of Ceres warrants further study and that this dwarf planet could be a valid target for future exploratory missions.

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Section: Discussionmentioning

confidence: 85%

Bacterial Utilisation of Aliphatic Organics: Is the Dwarf Planet Ceres Habitable?

Jayasinghe

Kennedy

McMinn

2022

Life

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“…a future focus of our work to determine whether SFB21 is a novel Acinetobacter spp. In fact, Acinetobacter strains, including SFB21 contain multiple copies of the fadE gene in their genomes, indicating a broad lipid catabolic capability [74], while Acinetobacter strains are known to grow well on acetate and palmitate [75–78], and to produce internal lipid-bodies for energy storage [79, 80]. One future focus however will be to understand under what conditions the Fad genes are expressed, i.e.…”

Section: Discussionmentioning

confidence: 99%

Looking through the FOG: microbiome characterization and lipolytic bacteria isolation from a fatberg site

et al. 2021

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Sewer systems are complex physical, chemical and microbial ecosystems where fats, oils and grease (FOG) present a major problem for sewer management. Their accumulation can lead to blockages (‘Fatbergs’), sewer overflows and disruption of downstream wastewater treatment. Further advancements of biological FOG treatments need to be tailored to degrade the FOG, and operate successfully within the sewer environment. In this study we developed a pipeline for isolation of lipolytic strains directly from two FOG blockage sites in the UK, and isolated a range of highly lipolytic bacteria. We selected the five most lipolytic strains using Rhodamine B agar plates and pNP-Fatty acid substrates, with two Serratia spp., two Klebsiella spp. and an environmental Acinetobacter strain that all have the capacity to grow on FOG-based carbon sources. Their genome sequences identified the genetic capacity for fatty acid harvesting (lipases), catabolism and utilization (Fad genes). Furthermore, we performed a preliminary molecular characterization of the microbial community at these sites, showing a diverse community of environmental bacteria at each site, but which did include evidence of sequences related to our isolates. This study provides proof of concept to isolation strategies targeting Fatberg sites to yield candidate strains with bioremediation potential for FOG in the wastewater network. Our work sets the foundation for development of novel bioadditions tailored to the environment with non-pathogenic Acinetobacter identified as a candidate for this purpose.

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“…strain H01-N also contains at least two ALDHs: one required for growth on short-chain alcohols (ALDH) and one for growth on long-chain alcohols and aldehydes. The role of fatty aldehyde dehydrogenases in the dissimilation of hexadecane, hexadecanol, and fatty aldehyde is discussed in the accompanying paper (16).…”

Section: I-mentioning

confidence: 99%

Alcohol dehydrogenases in Acinetobacter sp. strain HO1-N: role in hexadecane and hexadecanol metabolism

Singer¹,

Finnerty²

1985

J Bacteriol

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Multiple alcohol dehydrogenases (ADH) were demonstrated in Acinetobacter sp. strain HO1-N. ADH-A and ADH-B were distinguished on the basis of electrophoretic mobility, pyridine nucleotide cofactor requirement, and substrate specificity. ADH-A is a soluble, NAD-linked, inducible ethanol dehydrogenase (EDH) exhibiting an apparent Km for ethanol of 512 microM and a Vmax of 138 nmol/min. An ethanol-negative mutant (Eth1) was isolated which contained 6.5% of wild-type EDH activity and was deficient in ADH-A. Eth1 exhibited normal growth on hexadecane and hexadecanol. A second ethanol-negative mutant (Eth3) was acetaldehyde dehydrogenase (ALDH) deficient, having 12.5% of wild-type ALDH activity. Eth3 had threefold-higher EDH activity than the wild-type strain. ALDH is a soluble, NAD-linked, ethanol-inducible enzyme which exhibited an apparent Km for acetaldehyde of 50 microM and a Vmax of 183 nmol/min. Eth3 exhibited normal growth on hexadecane, hexadecanol, and fatty aldehyde. ADH-B is a soluble, constitutive, NADP-linked ADH which was active with medium-chain-length alcohols. Hexadecanol dehydrogenase (HDH), a soluble and membrane-bound, NAD-linked ADH, was induced 5- to 11-fold by growth on hexadecane or hexadecanol. HDH exhibited apparent Kms for hexadecanol of 1.6 and 2.8 microM in crude extracts derived from hexadecane- and hexadecanol-grown cells, respectively. HDH was distinct from ADH-A and ADH-B, since HDH and ADH-A were not coinduced; Eth1 had wild-type levels of HDH; and HDH requires NAD, while ADH-B requires NADP. NAD- and NADP-independent HDH activity was not detected in the soluble or membrane fraction of extracts derived from hexadecane- or hexadecanol-grown cells. NAD-linked HDH appears to possess a functional role in hexadecane and hexadecanol dissimilation.

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Fatty aldehyde dehydrogenases in Acinetobacter sp. strain HO1-N: role in hexadecanol metabolism

Cited by 25 publications

References 16 publications

Bacterial Utilisation of Aliphatic Organics: Is the Dwarf Planet Ceres Habitable?

Bacterial Utilisation of Aliphatic Organics: Is the Dwarf Planet Ceres Habitable?

Looking through the FOG: microbiome characterization and lipolytic bacteria isolation from a fatberg site

Alcohol dehydrogenases in Acinetobacter sp. strain HO1-N: role in hexadecane and hexadecanol metabolism

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