Fatty acyl-CoA reductase influences wax biosynthesis in the cotton mealybug, Phenacoccus solenopsis Tinsley

Tong, Haojie; Wang, Yuan; Wang, Shuping; Omar, Mohamed A.A.; Li, Zicheng; Li, Zihao; Ding, Simin; Ao, Yan; Wang, Ying; Li, Fei; Jiang, Mingxing

doi:10.1038/s42003-022-03956-y

Cited by 13 publications

(8 citation statements)

References 63 publications

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“…RNAi-mediated suppression of FAR expression in the cotton mealybug, Phenacoccus solenopsis Tinsley, resulted in reduced CHC levels in the wax layer [54]. Mealybugs with reduced CHC content exhibited increased mortality when exposed to desiccation and deltamethrin treatments, highlighting the importance of this enzyme for insect adaptation to water loss and insecticide stress.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and Metatranscriptomic Analyzes Provide New Insights into the Response of the Pea Aphid <em>Acyrthosiphon pisum</em> (Hemiptera: Aphididae) to Acetamiprid

Cai,

Zhao,

Qian

et al. 2024

Preprint

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Acetamiprid is a broad-spectrum neonicotinoid insecticide used in agriculture to control aphids. While recent studies have documented resistance to acetamiprid in several aphid species, the underlying mechanisms are still not fully understood. In this study, we analyzed the transcriptome and metatranscriptome of a laboratory strain of the pea aphid, Acyrthosiphon pisum (Harris, 1776), with reduced susceptibility to acetamiprid after nine generations of exposure to identify candidate genes and the microbiome involved in the adaptation process. Sequencing of the transcriptome of both selected (RS) and non-selected (SS) strains allowed the identification of 14858 genes and 4938 new transcripts. Most of the differentially expressed genes were associated with catalytic activities and metabolic pathways involving carbon and fatty acids. Specifically, the alcohol-forming fatty acyl-CoA reductase (FAR) and acyl-CoA synthetase (ACSF2), both involved in the synthesis of epidermal wax layer components, were significantly upregulated in RS, suggesting that adaptation to acetamiprid involves the synthesis of a thicker protective layer. Metatranscriptomic analysis revealed subtle shifts in the microbiome of RS. These results contribute to a deeper understanding of acetamiprid adaptation by the pea aphid and provide new insights for aphid control strategies.

show abstract

Section: Discussionmentioning

confidence: 99%

Transcriptomic and Metatranscriptomic Analyzes Provide New Insights into the Response of the Pea Aphid <em>Acyrthosiphon pisum</em> (Hemiptera: Aphididae) to Acetamiprid

Cai,

Zhao,

Qian

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…RNAi-mediated suppression of FAR expression in the cotton mealybug, Phenacoccus solenopsis Tinsley, resulted in reduced CHC levels in the wax layer [55]. Mealybugs with reduced CHC content exhibited increased mortality when exposed to desiccation and deltamethrin treatments, highlighting the importance of this enzyme for insect adaptation to water loss and insecticide stress.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and Metatranscriptomic Analyses Provide New Insights into the Response of the Pea Aphid Acyrthosiphon pisum (Hemiptera: Aphididae) to Acetamiprid

Cai,

Zhao,

Qian

et al. 2024

Insects

View full text Add to dashboard Cite

Acetamiprid is a broad-spectrum neonicotinoid insecticide used in agriculture to control aphids. While recent studies have documented resistance to acetamiprid in several aphid species, the underlying mechanisms are still not fully understood. In this study, we analyzed the transcriptome and metatranscriptome of a laboratory strain of the pea aphid, Acyrthosiphon pisum (Harris, 1776), with reduced susceptibility to acetamiprid after nine generations of exposure to identify candidate genes and the microbiome involved in the adaptation process. Sequencing of the transcriptome of both selected (RS) and non-selected (SS) strains allowed the identification of 14,858 genes and 4938 new transcripts. Most of the differentially expressed genes were associated with catalytic activities and metabolic pathways involving carbon and fatty acids. Specifically, alcohol-forming fatty acyl-CoA reductase (FAR) and acyl-CoA synthetase (ACSF2), both involved in the synthesis of epidermal wax layer components, were significantly upregulated in RS, suggesting that adaptation to acetamiprid involves the synthesis of a thicker protective layer. Metatranscriptomic analyses revealed subtle shifts in the microbiome of RS. These results contribute to a deeper understanding of acetamiprid adaptation by the pea aphid and provide new insights for aphid control strategies.

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“…In the present study, O. furnacalis PGRPA may possess similar functions with other orthologs. In Lepidoptera, XM_028315055.1 ( OfCG5065‐1 ), which is a fatty acyl‐CoA reductase ( FAR ),L can affect the synthesis of pheromones (Tong et al, 2022). For instance, HzeaFAR1 identified in H. armigera has been proven to be able to synthesize wax to isolate foreign infestations (Dou et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of immune‐related genes of Asian corn borer (Ostrinia furnacalis [Guenée]) after oral bacterial infection

Qiao

Mei

et al. 2023

Arch Insect Biochem Physiol

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The Asian corn borer (Ostrinia furnacalis) is an important agricultural pest causing serious damage to economic crops, such as corn and sorghum. The gut is the first line of defense against pathogens that enter through the mouth. Staphylococcus aureus was used to infect the O. furnacalis midgut to understand the midgut immune mechanism against exogenous pathogens to provide new ideas and methods for the prevention and control of O. furnacalis. A sequencing platform was used for genome assembly and gene expression. The unigene sequences were annotated and functionally classified by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes. Significant differences were found in the induced expression profiles before and after infection. Some differentially expressed genes have important relations with lipid metabolism and immune mechanism, suggesting that they play an important role in the innate immune response of O. furnacalis. Furthermore, quantitative real‐time polymerase chain reaction assay was used to identify the key genes involved in the signaling pathway, and the expression patterns of these key genes were confirmed. The results could help study the innate immune system of lepidopteran insects and provide theoretical support for the control of related pests and the protection of beneficial insects.

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Fatty acyl-CoA reductase influences wax biosynthesis in the cotton mealybug, Phenacoccus solenopsis Tinsley

Cited by 13 publications

References 63 publications

Transcriptomic and Metatranscriptomic Analyzes Provide New Insights into the Response of the Pea Aphid <em>Acyrthosiphon pisum</em> (Hemiptera: Aphididae) to Acetamiprid

Transcriptomic and Metatranscriptomic Analyzes Provide New Insights into the Response of the Pea Aphid <em>Acyrthosiphon pisum</em> (Hemiptera: Aphididae) to Acetamiprid

Transcriptomic and Metatranscriptomic Analyses Provide New Insights into the Response of the Pea Aphid Acyrthosiphon pisum (Hemiptera: Aphididae) to Acetamiprid

Transcriptome analysis of immune‐related genes of Asian corn borer (Ostrinia furnacalis [Guenée]) after oral bacterial infection

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