Fatty acids of erythrocyte membrane in acute pancreatitis patients

Kuliavienė, Irma; Gulbinas, Antanas; Cremers, Johannes W. J. M.; Pundzius, Juozas; Kupčinskas, Limas; Dambrauskas, Žilvinas; Jansen, Eugène

doi:10.3748/wjg.v19.i34.5678

Cited by 9 publications

(4 citation statements)

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“…The peroxidation of polyunsaturated fatty acids at the cell membrane, induced by an excess of free radicals [37] , causes damage and cell death [38] with the release of malondialdehyde (MDA), a byproduct derived from lipid peroxidation [6] . After a 180-min incubation of erythrocytes with E-Jds, a dose-dependent reduction of MDA generation was observed ( Figure 3 ).…”

Section: Resultsmentioning

confidence: 99%

Antioxidant and Cytotoxic Activity of Hydroethanolic Extract from Jacaranda decurrens Leaves

et al. 2014

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Background and PurposeLeaves of Jacaranda decurrens are used in traditional Brazilian medicine to treat metabolic diseases related to increased reactive oxygen species. The present study evaluated the antioxidant and cytotoxic potential of hydroethanolic extract from the leaves of Jacaranda decurrens subsp. symmetrifoliolata.Experimental ApproachPhenolic compounds, flavonoids and saponins were evaluated in an ethanol∶water (80∶20, v/v) extract from the leaves of Jacaranda decurrens subsp. symmetrifoliolata (E-Jds). The antioxidant activity of E-Jds was investigated by assessing the following: 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity; protection against 2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced hemolysis of erythrocytes; in vitro and in vivo malondialdehyde dosage; and the ability to activate antioxidant enzymes. K562 leukemia cells were used for the cytotoxic evaluation of E-Jds and for the assessment of the cell death profile through flow cytometry.Key ResultsPhenolic and flavonoid compounds were quantified as 14.38% and 2.15%, respectively, of E-Jds. These phenolic and flavonoid compounds proved to be able to scavenge DPPH free radicals with an IC50 of 9.3±3.3 µg/mL, to protect up to 50% of erythrocytes against AAPH-induced hemolysis and to reduce in vitro and in vivo malondialdehyde levels up to 84% and 22%, respectively. E-Jds also increased glutathione peroxidase enzyme activity, with a concomitant decrease in superoxide dismutase and catalase activity, and exhibited dose-dependent cytotoxic activity on K562 erythroleukemia cells with cell death occurring via both late apoptosis and necrosis.ConclusionsE-Jds exhibits in vitro and in vivo antioxidant potential, which may be the mechanism mediating the metabolic activities reported in folk medicine. Furthermore, the cytotoxic activity identified in this study contributes with the knowledge of antiproliferative activities that have been described in the literature for the genus Jacaranda.

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Section: Resultsmentioning

confidence: 99%

Antioxidant and Cytotoxic Activity of Hydroethanolic Extract from Jacaranda decurrens Leaves

et al. 2014

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“…The antioxidant activity of AmT was demonstrated in vitro by the direct scavenging of ABTS and DPPH reactive species, the inhibition of lipid peroxidation induced by AAPH, and the reduction of oxidative hemolysis and malondialdehyde production in human erythrocytes. The peroxidation of polyunsaturated fatty acids in the cell membrane, which is induced by excess free radicals [ 47 ], causes damage and cell death [ 48 ] and the release of malondialdehyde (MDA), a byproduct derivative of lipid peroxidation [ 49 ]. Our results suggest a synergy between compounds with antioxidant properties obtained from macerated whole bees compared to synthetic antioxidants, ascorbic acid, and BHT.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant, antihyperglycemic, and antidiabetic activity of Apis mellifera bee tea

et al. 2018

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Diabetes has emerged as one of the largest global epidemics; it is estimated that by 2035, there will be 592 million diabetic people in the world. Brazilian biodiversity and the knowledge of traditional peoples have contributed to the treatment of several diseases, including diabetes. Apis mellifera bee tea is used by indigenous Brazilians to treat diabetes, and this traditional knowledge needs to be recorded and studied.The objective of this study was to record the use and to evaluate the antioxidant, antihyperglycemic, and antidiabetic activity of Apis mellifera bee tea, which is used by the Guarani and Kaiowá indigenous people for the treatment of diabetes. Semi-structured interviews were performed with Guarani and Kaiowá ethnic indigenous people from the State of Mato Grosso do Sul, Brazil, seeking to identify the animal species used for medicinal purposes. For the experimental procedures, tea prepared with macerated Apis mellifera bees was used. In vitro assays were performed to evaluate antioxidant activity; direct free radical scavenging, protection against oxidative hemolysis, lipid peroxidation were evaluated in human erythrocytes and potential in inhibiting the formation of advanced glycation end products (AGEs). In vivo, normoglycemic Swiss male mice treated with Apis mellifera tea (AmT) were subjected to the oral glucose tolerance test and compared with control and metformin-treated groups. Diet-induced diabetic mice were treated for 21 days with AmT and evaluated for glycemia and malondialdehyde levels in the blood, liver, nervous system, and eyes. During interviews, the indigenous people described the use of Apis mellifera bee tea for the treatment of diabetes. In in vitro assays, AmT showed direct antioxidant activity and reduced oxidative hemolysis and malondialdehyde generation in human erythrocytes. The AmT inhibited the formation of AGEs by albumin-fructose pathways and methylglyoxal products. In vivo, after oral glucose overload, normoglycemic mice treated with AmT had reduced hyperglycemia at all times evaluated up to 180 min. AmT also reduced hyperglycemia and malondialdehyde levels in the blood, liver, nervous system, and eyes of diabetic mice to similar levels as those in metformin-treated mice and normoglycemic controls. In summary, Apis mellifera bee tea showed antioxidant, antihyperglycemic, and antidiabetic activity, which provides support for the therapeutic application of Guarani and Kaiowá indigenous knowledge.

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“…The peroxidation of the phospholipid membrane is induced by reactive species, especially oxygen and nitrogen species, causing cell lysis and death. Furthermore, a byproduct of this reaction is malondialdehyde [65, 66], which is a toxic product leading to the release of unsaturated fatty acids and the fragmentation of phospholipids, causing mutations and cellular rupture [19]. The EEB and EEL reduced the generation of malondialdehyde during the early evaluation times, contributing to the maintenance of a cellular structure, at low concentrations.…”

Section: Discussionmentioning

confidence: 99%

Chemical Composition, Antimicrobial Activity, and Antioxidant Activity of Ocotea minarum (Nees & Mart.) Mez.

Rodrigues

Almeida-Apolonio

Alfredo

et al. 2019

Oxidative Medicine and Cellular Longevity

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Ocotea minarum is a native plant from Brazil, popularly known as “canelinha” or “canela vassoura.” The objective of this study was to investigate the chemical composition of the extracts of the bark and the leaves of O. minarum and to evaluate its antimicrobial and antioxidant activities. The phenolic compounds, flavonoids and tanins, were quantified with the reagents Folin-Ciocalteu, aluminium chloride, and vanillin. The chemical profile was performed by HPLC-DAD. The minimum inhibitory concentration was evaluated by the microdilution in a broth method. The antioxidant activity was measured by the capture of free radicals 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid). In addition, protection against oxidative hemolysis and generation of malondialdehyde were evaluated in human erythrocytes. The composition of the extracts included the caffeic acid, p-coumaric acid, and rosmarinic acid, besides the flavonoids quercetin and luteolin. The EEL showed bacteriostatic action of 1000 μg/mL for all evaluated Salmonella Typhimurium, Salmonella Enteritidis, Pseudomonas aeruginosa, and Proteus mirabilis, and the EHEB had a moderate antifungal action against Candida krusei and Cryptococcus gattii (250 μg/mL). IC50 values of 8.19 (EEL) and 4.51 μg/mL (EEB) in the assay with DPPH and 6.25 (EEL) and 2.87 μg/mL (EEB) in the assay with ABTS were obtained. Up to the 3rd hour of oxidative hemolysis testing induced by AAPH, the EEB and EEL had a protective action, reducing the malondialdehyde. In conclusion, the data indicate that the O. minarum extracts can be evaluated as bioactive supplies for the development of new drugs for the prevention and treatment of diseases related to oxidative stress and microbial infections.

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Fatty acids of erythrocyte membrane in acute pancreatitis patients

Abstract: The composition of FAs in erythrocyte membranes is altered during AP. These changes are likely to be associated with alcohol consumption, inflammatory processes, and oxidative stress.

Cited by 9 publications

References 34 publications

Antioxidant and Cytotoxic Activity of Hydroethanolic Extract from Jacaranda decurrens Leaves

Antioxidant and Cytotoxic Activity of Hydroethanolic Extract from Jacaranda decurrens Leaves

Antioxidant, antihyperglycemic, and antidiabetic activity of Apis mellifera bee tea

Chemical Composition, Antimicrobial Activity, and Antioxidant Activity of Ocotea minarum (Nees & Mart.) Mez.

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