Fatty acids in forages: A comparison of different pre-treatments prior to analysis

Arvidsson, Katarina; Gustavsson, Anne‐Maj; Martinsson, Κjell

doi:10.1016/j.anifeedsci.2008.12.003

Cited by 10 publications

(7 citation statements)

References 15 publications

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“…In keeping with previous observations by Arvidsson 318 K. Arvidsson et al et al (2009), the variation was more pronounced during summer growth (Table I). This could be due to variation in the physiological status of individual plants.…”

Section: Seasonal Variationsupporting

confidence: 91%

“…These contrasting results could be due to differences in the plant materials studied and their degree of maturation. Arvidsson et al (2009) compared different handling methods and did not find any substantial differences between, for example, drying in an air forced oven, freezing at (208C, or freeze drying, which indicates that differences in sample preparation should not contribute significantly to any differences between studies in terms of measured FA composition.…”

Section: Seasonal Variationmentioning

confidence: 83%

See 1 more Smart Citation

Fatty acid concentrations in timothy (Phleum pratenseL.) and meadow fescue (Festuca pratensisHuds.): Effects of seasonal variations at different nitrogen fertilization levels

Arvidsson¹,

Martinsson²,

Gustavsson³

2013

Acta Agriculturae Scandinavica, Section B - Soil & Plant Sc

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Section: Seasonal Variationsupporting

confidence: 91%

Section: Seasonal Variationmentioning

confidence: 83%

Fatty acid concentrations in timothy (Phleum pratenseL.) and meadow fescue (Festuca pratensisHuds.): Effects of seasonal variations at different nitrogen fertilization levels

Arvidsson¹,

Martinsson²,

Gustavsson³

2013

Acta Agriculturae Scandinavica, Section B - Soil & Plant Sc

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“…Rapid freezing with dry ice or liquid N 2 followed by storage at −20°C in an inert (N 2 ) atmosphere has been considered to be the best method to preserve plant tissues (Christie, ). However, comparison of various techniques showed limited effects on fatty acid proportions, and drying of samples has been shown to be an adequate method for preparing samples for fatty acid analysis in samples collected in diverse experiments, both field and feeding experiments, which were not specially designed to investigate fatty acid concentrations (Arvidsson et al ., ). Five grams of the dried sample were used for total fat extraction and fatty acid analysis.…”

Section: Methodsmentioning

confidence: 97%

Influence of cultivar and cutting date on the fatty acid composition of forage crops for grazing beef production in Argentina

García

Pordomingo²,

Pérez

et al. 2015

Grass and Forage Science

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The fatty acid composition and total lipids of various forage crops at different cutting dates and seasons, which are used in forage grazing beef production in Argentina, were determined. Three experiments were carried out at the Agricultural Experimental Station of INTA, Anguil, La Pampa, Argentina Samples of alfalfa from three different cultivars with different dormancy groups (G4-5, G6-7 and G8-9) and samples of ryegrasses Bill, Florida and Queue, wheats Charrua and Guapo, and triticale Don Santiago cultivars were taken at different cutting dates and analysed for total lipids and fatty acid composition. Significant differences on the fatty acid composition, the ratios 18:3n-3/18:2n-6 and polyunsaturated fatty acids (PUFA) due to cultivar, cutting date and season were found. Taking into account the importance of 18:2n-6 and 18:3n-3 fatty acids as substrates for the conjugated isomers of 18:2n-6 (CLA) and n-3 PUFA concentrations in beef, the fatty acid composition of forages needs to be considered in forage grazing beef production systems. Data variation in both total lipids and individual fatty acid proportions in forages should be taken into account to establish the potential to cultivate crops for a higher content of n-3 PUFA and hence the opportunity to deliver more n-3 PUFA and CLA into beef.

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“…Ground samples were then stored in a dry, dark area until transported to the Nutritional Ecology Laboratory at Hunter College, New York, USA and stored in a dry, dark area until assayed [Rothman et al, ]. In addition, because unsaturated fatty acids are susceptible to oxidation [Czerkawski, ; Molloy et al, ; but see: Arvidsson et al, ], on January 23, 2013, 1–3 g of each food species was recollected in BINP and were immediately placed in liquid nitrogen in 5 ml cryotubes for fatty acid analysis.…”

Section: Methodsmentioning

confidence: 99%

Fatty acids in mountain gorilla diets: Implications for primate nutrition and health

Reiner

Petzinger

Power

et al. 2013

American J Primatol

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Little is known about the fatty acid composition of foods eaten by wild primates. A total of 18 staple foods that comprise 97% of the annual dietary intake of the mountain gorilla (Gorilla beringei) were analyzed for fatty acid concentrations. Fruits and herbaceous leaves comprise the majority of the diet, with fruits generally having a higher mean percentage of fat (of dry matter; DM), as measured by ether extract (EE), than herbaceous leaves (13.0% ± SD 13.0% vs. 2.3 ± SD 0.8%). The mean daily EE intake by gorillas was 3.1% (DM). Fat provided ≈14% of the total dietary energy intake, and ≈22% of the dietary non-protein energy intake. Saturated fatty acids accounted for 32.4% of the total fatty acids in the diet, while monounsaturated fatty acids accounted for 12.5% and polyunsaturated fatty acids (PUFA) accounted for 54.6%. Both of the two essential PUFA, linoleic acid (LA, n-6) and α-linolenic acid (ALA, n-3), were found in all of the 17 staple foods containing crude fat and were among the three most predominant fatty acids in the diet: LA (C18:2n-6) (30.3%), palmitic acid (C16:0) (23.9%), and ALA (C18:3n-3) (21.2%). Herbaceous leaves had higher concentrations of ALA, while fruit was higher in LA. Fruits provided high amounts of fatty acids, especially LA, in proportion to their intake due to the higher fat concentrations; despite being low in fat, herbaceous leaves provided sufficient ALA due to the high intake of these foods. As expected, we found that wild mountain gorillas consume a diet lower in EE, than modern humans. The ratio of LA:ALA was 1.44, closer to agricultural paleolithic diets than to modern human diets.

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Fatty acids in forages: A comparison of different pre-treatments prior to analysis

Cited by 10 publications

References 15 publications

Fatty acid concentrations in timothy (Phleum pratenseL.) and meadow fescue (Festuca pratensisHuds.): Effects of seasonal variations at different nitrogen fertilization levels

Fatty acid concentrations in timothy (Phleum pratenseL.) and meadow fescue (Festuca pratensisHuds.): Effects of seasonal variations at different nitrogen fertilization levels

Influence of cultivar and cutting date on the fatty acid composition of forage crops for grazing beef production in Argentina

Fatty acids in mountain gorilla diets: Implications for primate nutrition and health

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