Fatty Acids from Hermetia illucens Larvae Fat Inhibit the Proliferation and Growth of Actual Phytopathogens

Marusich, Elena; Mohamed, Heakal; Afanasev, Yuriy; Leonov, Sergey

doi:10.3390/microorganisms8091423

Cited by 22 publications

(22 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is in accordance with the hypothesis that fatty acids are probably involved in the mechanism of action of the oils tested in this study. In fact, Gram-positive bacteria have a cell wall constituted by a single thick peptidoglycan layer only, while Gram-negative bacteria, although characterized by a thin peptidoglycan layer, present an outer membrane layer composed of lipoproteins, lipopolysaccharides, and phospholipids [ 45 ]. This layer of Gram-negative bacteria prevents the entry of intermediate and long-chain fatty acids and their subsequent toxic action [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

Mechanical Processing of Hermetia illucens Larvae and Bombyx mori Pupae Produces Oils with Antimicrobial Activity

Saviane

Tassoni

Lupi

et al. 2021

Animals

View full text Add to dashboard Cite

The aim of this work was to develop processing methods that safeguard the quality and antimicrobial properties of H. illucens and B. mori oils. We adopted a vegetable diet for both insects: leftover vegetables and fruit for H. illucens and mulberry leaves for B. mori. First, alternative techniques to obtain a good oil extraction yield from the dried biomass of H. illucens larvae were tested. Traditional pressing resulted to be the best system to maximize the oil yield and it was successfully applied to B. mori pupae. Oil quality resulted comparable to that obtained with other extraction methods described in the literature. In the case of B. mori pupae, different treatments and preservation periods were investigated to evaluate their influence on the oil composition and quality. Interestingly, agar diffusion assays demonstrated the sensitivity of Gram-positive Bacillus subtilis and Staphylococcus aureus to H. illucens and B. mori derived oils, whereas the growth of Gram-negative Pseudomonas aeruginosa and Escherichia coli was not affected. This study confirms that fat and other active compounds of the oil extracted by hot pressing could represent effective antimicrobials against bacteria, a relevant result if we consider that they are by-products of the protein extraction process in the feed industry.

show abstract

Section: Discussionmentioning

confidence: 99%

Mechanical Processing of Hermetia illucens Larvae and Bombyx mori Pupae Produces Oils with Antimicrobial Activity

Saviane

Tassoni

Lupi

et al. 2021

Animals

View full text Add to dashboard Cite

show abstract

“…As described in our previous work, the bioactive compounds from AWME extract received using our developed protocol of BSFL fat extraction demonstrated a high level of antimicrobial activity against a broad spectrum of bacterial phytopathogens [47]. In attempt to improve the extraction procedure, we applied three rounds of sequential extractions of the same biomass of BSFL fat using the same protocol based on acidic water-methanol extraction solution (90:9:1 v/v%) in order to get the set of sequential extracts (SEs) named as AWME1, AWME2, and AWME3 (Figure 1).…”

Section: Sequential Extractionmentioning

confidence: 64%

“…Besides, BSFL fat is rich in mediumchain lauric acid with known antimicrobial activity towards the disruption of the bacterial cell membrane [46]. Recently we demonstrated the substantial antibacterial potential of acidic water-methanol extracts (AWME) of H. illucens larvae fat against contemporary phytopathogens [47].…”

Section: Introductionmentioning

confidence: 99%

Fatty Acids-Enriched Fractions of Hermetia illucens (Black Soldier Fly) Larvae Fat Can Combat MDR Pathogenic Fish Bacteria Aeromonas spp.

Mohamed

Marusich

Afanasev

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

Aeromonas spp. cause many diseases in aquaculture habitats. Hermetia illucens (Hi) larvae were used as feed-in aquacultures and in eradicating pathogenic fish bacteria. In the present study, we applied consecutive extractions of the same biomass of BSFL fat using the acidic water–methanol solution. The major constituents of the sequential extracts (SEs) were free fatty acids (FFAs), and fatty acids derivatives as identified by gas chromatography spectrometry (GC-MS). Our improved procedure enabled gradual enrichment in the unsaturated fatty acids (USFAs) content in our SEs. The present study aimed to compare the composition and antimicrobial properties of SEs. Among actual fish pathogens, A. hydrophila and A. salmonicida demonstrated multiple drug resistance (MDR) against different recommended standard antibiotics: A. salmonicida was resistant to six, while A. hydrophila was resistant to four antibiotics from ten used in the present study. For the first time, we demonstrated the high dose-dependent antibacterial activity of each SE against Aeromonas spp., especially MDR A. salmonicida. The bacteriostatic and bactericidal (MIC/MBC) activity of SEs was significantly enhanced through the sequential extractions. The third sequential extract (AWME3) possessed the highest activity against Aeromonas spp.: inhibition zone diameters were in the range (21.47 ± 0.14–20.83 ± 0.22 mm) at a concentration of 40 mg/mL, MIC values ranged between 0.09 and 0.38 mg/mL for A. hydrophila and A. salmonicida, respectively. AWME3 MBC values recorded 0.19 and 0.38 mg/mL, while MIC50 values were 0.065 ± 0.004 and 0.22 ± 0.005 mg/mL against A. hydrophila and A. salmonicida, respectively. Thus, the larvae fat from Hermitia illucens may serve as an excellent reservoir of bioactive molecules with good capacity to eradicate the multidrug-resistant bacteria, having promising potential for practical application in the aquaculture field.

show abstract

“…The antioxidant properties of the UFAs, namely, oleic [ 12 ], linoleic [ 61 ], and stearic [ 12 ] acids, are very important both for topical application and for ingestion because oxidative damage occurs at both the tissue and systemic levels [ 62 ]. Studies examining the antioxidant activity of oils obtained from insects is scarce in the literature [ 63 – 66 ]; however, the antioxidant activity of RPLO was similar to vegetable oils shown to be beneficial to health, such as sunflower, macadamia, linseed, and chia oils [ 67 ].…”

Section: Discussionmentioning

confidence: 99%

Rhynchophorus palmarum (Linnaeus, 1758) (Coleoptera: Curculionidae): Guarani-Kaiowá indigenous knowledge and pharmacological activities

et al. 2021

View full text Add to dashboard Cite

Zootherapy is a traditional secular practice among the Guarani-Kaiowá indigenous ethnic group living in Mato Grosso do Sul, Brazil. My people use the oil extracted from larvae of the snout beetle Rhynchophorus palmarum (Linnaeus, 1758) to treat and heal skin wounds and respiratory diseases. Based on this ethnopharmacological knowledge, the chemical composition and antioxidant, antimicrobial, and healing properties of R. palmarum larvae oil (RPLO) were investigated, as well as possible toxic effects, through in vitro and in vivo assays. The chemical composition of the RPLO was determined using gas chromatography coupled with mass spectrometry. The antioxidant activity of RPLO was investigated through the direct 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, and the antimicrobial activity was evaluated against Gram-positive and Gram-negative bacteria that are pathogenic to humans. The healing properties of RPLO were investigated by performing a cell migration assay using human lung fibroblasts (MRC-5), and the toxicity was analyzed, in vivo, using a Caenorhabditis elegans model and MRC-5 cells, in vitro. RPLO contains 52.2% saturated fatty acids and 47.4% unsaturated fatty acids, with palmitic acid (42.7%) and oleic acid (40%) representing its major components, respectively. RPLO possesses direct antioxidant activity, with a half-maximal inhibitory concentration (IC50) of 46.15 mg.ml-1. The antimicrobial activity of RPLO was not observed at a concentration of 1% (v/v). RPLO did not alter the viability of MRC-5 cells and did not exert toxic effects on C. elegans. Furthermore, MRC-5 cells incubated with 0.5% RPLO showed a higher rate of cell migration than that of the control group, supporting its healing properties. Taken together, RPLO possesses direct antioxidant activity and the potential to aid in the healing process and is not toxic toward in vitro and in vivo models, corroborating the safe use of the oil in traditional Guarani-Kaiowá medicine.

show abstract

Fatty Acids from Hermetia illucens Larvae Fat Inhibit the Proliferation and Growth of Actual Phytopathogens

Cited by 22 publications

References 68 publications

Mechanical Processing of Hermetia illucens Larvae and Bombyx mori Pupae Produces Oils with Antimicrobial Activity

Mechanical Processing of Hermetia illucens Larvae and Bombyx mori Pupae Produces Oils with Antimicrobial Activity

Fatty Acids-Enriched Fractions of Hermetia illucens (Black Soldier Fly) Larvae Fat Can Combat MDR Pathogenic Fish Bacteria Aeromonas spp.

Rhynchophorus palmarum (Linnaeus, 1758) (Coleoptera: Curculionidae): Guarani-Kaiowá indigenous knowledge and pharmacological activities

Contact Info

Product

Resources

About