Fatty Acids Composition of Tocte (Juglans Neotropica Diels) Walnut From Ecuador

Abstract

Objective: The aim of this study was to determine the fatty acids composition in a tocte seeds oil (Juglans neotropica Diels) sample cultivated in Ecuador.Methods: Tocte oil was obtained from tocte seeds using the cold pressing method. Fatty acids analysis was carried out using the gas chromatography method with a mass selective detector (GC/MSD) and using the database Library NIST14.L to identify the compounds. Results:Methyl esters fatty acids were identified from tocte (J. neotropica Diels) walnut using the GC-MS analytical method. The total lipid content of tocte walnuts seeds of plants cultivated in Ecuador was of 49.01% of the total lipid content on fresh weight. Fatty acids were analyzed as methyl esters on a capillary column DB-WAX 122-7062 with a good separation of palmitic acid, stearic acid, oleic acid, linoleic acid, and linolenic acid. The structure of methyl esters fatty acids was determined using the GC-MS. Tocte walnut presents 5.05% of palmitic acid, 2.26% of stearic acid, 19.50% of oleic acid, 65.81% of linoleic acid, and 2.79% linolenic acid of the total content of fatty acids in tocte oil. Fatty acids content reported in this study were similar to the data reported for other walnuts seeds.Conclusions: Tocte seeds are a good source of monounsaturated and polyunsaturated fatty acids. Tocte oil content oleic acid and with a good content of ɷ6 α-linoleic and ɷ3 α-linolenic. Tocte walnut can help reduce risk cardiovascular diseases in Ecuador for their good composition of fatty acids.

Comparison of Methods of External and Internal Standard for Gs Determination of Docosahexaenoic Acid

“…The determination of DHA and EPA in fish oil has been reported by high-performance liquid chromatography (HPLC) (Dillon et al 2013) and HPLC with mass spectrometry (MS) detector (Lacaze et al 2017;Salm et al 2011). For simultaneous determination of DHA, EPA and arachidonic acid in human plasma, HPLC with electrochemical detector using the reversephase C30 column and detection based on the voltammetric reduction of 3,5-di-tert-butyl-1,2benzoquinone is used (Kotani et al 2016 (Vilcacundo et al 2018). GC/MS is reported with a highly polar capillary column (CP-Sil 88, 100 m) for simultaneously determination of DHA and EPA as active and fish oil nutritional capsules.…”