Fatty acids and its derivatives of Acorus calamus Linn. rhizome induce stem cell-mediated cell division in plants and animals

Rajesh, Chandran; Palanimuthu, Vasanth Raj; Palanichelvam, Karuppaiah

doi:10.1016/j.bcab.2021.102153

Cited by 3 publications

(1 citation statement)

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“…Later it was xed and stained with safranin stain and viewed in the light microscope. The mitotic index was calculated as mentioned earlier (Rajesh et al 2021).…”

Section: 3allium Cepa Root Tip Assaymentioning

confidence: 99%

Three Antimitotic Compounds Found in Banana Pseudo-Stem By Using Earthworms.

Rajesh

Balaji

Prakash

et al. 2021

Preprint

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To identify antimitotic compounds from abundant and inexpensive plant resources, banana pseudo-stem was (BPS) chosen. Onion root tip assay and earthworm regeneration assay were carried out to test theantimitotic potential of aqueous extract of BPS.Earthworm (Eudrilus eugeniae) regeneration assay exploits the regeneration ability of amputated earthworms that retain the clitellum region. Aqueous extract of BPS decreased the mitotic index in Allium cepa root tips. Besides, thisaqueous extract of BPS inhibited the regeneration of blastema from amputated earthworms as well. Validation of this extract with MTT (3-(4,5-dimethyl thiazolyl-2-yl)- 2,5-diphenyltetrazolium bromide) assay using MCF-7 breast cancer cell linefurther supported the presence of antimitotic compounds. Aqueous BPS extract was further fractionated with ethyl acetateand it was found to inhibit the regeneration of new tissues from amputated earthworms. Liquid Chromatography and Mass spectrometry (LC-MS) analysis was performed with aqueousBPS extract to predict the lead compounds.Prediction analysis with mass values revealed the presence of three different compounds viz. α-tocotrienol, 1,2,4-nonadecanetriol and 3',4',7-trihydroxyisoflavone, which were already reported to inhibit the cell division. All our results strongly supported that banana pseudo-stem extract possesses antimitotic compounds. This is the first report of identification of putative antimitotic compounds from aqueous extract of Musa paradisiaca var. Robusta by using earthworms.

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“…Later it was xed and stained with safranin stain and viewed in the light microscope. The mitotic index was calculated as mentioned earlier (Rajesh et al 2021).…”

Section: 3allium Cepa Root Tip Assaymentioning

confidence: 99%