Fatty acids and fatty aldehydes of buffalo seminal plasma and sperm lipid

Jain, Y. C.; Anand, Shashi

doi:10.1530/jrf.0.0470261

Cited by 32 publications

(10 citation statements)

References 18 publications

(23 reference statements)

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“…The methyl esters were prepared by BF3-methanol reagent as described by Jain & Anand (1976b). These were run on a dual-column gas Chromatograph (No.…”

Section: Methodsmentioning

confidence: 99%

Phospholipase and lysophospholipase activities of goat spermatozoa in transit from the caput to the cauda epididymidis

Atreja¹,

Anand²

1985

Reproduction

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Phospholipase and lysophospholipase activities were assayed in goat epididymal spermatozoa. Lysophospholipase was 10 times more active than phospholipase, and both enzymes decreased in activity substantially in the transit of spermatozoa from the caput to the cauda epididymidis. A comparative study revealed that phosphatidyl-ethanolamine, -choline and -inositol and phosphatidic acid were hydrolysed by goat sperm phospholipase. Hydrolysis of phosphatidylethanolamine/phosphatidylcholine revealed the end products to be glycerophosphoethanolamine/choline but neither diglycerides nor lysophosphatidylethanolamine/lysophosphatidylcholine were detected.

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“…The methyl esters were prepared by BF3-methanol reagent as described by Jain & Anand (1976b). These were run on a dual-column gas Chromatograph (No.…”

Section: Methodsmentioning

confidence: 99%

Phospholipase and lysophospholipase activities of goat spermatozoa in transit from the caput to the cauda epididymidis

Atreja¹,

Anand²

1985

Reproduction

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“…Phospholipid content accounts for the highest ratio in total lipid of spermatozoa and varies among species (Table 1). The phospholipid content of water buffalo spermatozoa and seminal plasma is 0.548 mg/10 9 cells and 0.594 mg/mL, respectively whereas these values in bull are 0.416 mg/10 9 and 1.491 mg/mL, respectively [5]. …”

Section: Biological Roles Of Lipid and Polyunsaturated Fatty Acids Inmentioning

confidence: 99%

“…In most mammals, DHA (C22:6 n-3) is the dominant PUFA, although, in several species, DPA (C22:5 n-3) predominates. Jain and Anand [5] observed that buffalo spermatozoa contained nearly equal distribution of saturated (47.8%) and unsaturated (49.8%) fatty acids and there were high levels of PUFA. More than 70 percent of the total unsaturated fatty acids were PUFA, mainly DHA (C22:6n-3) except in the neutral lipids where it was arachidonic acid (C20:4n-6).…”

Section: Biological Roles Of Lipid and Polyunsaturated Fatty Acids Inmentioning

confidence: 99%

“…According to Kadirvel et al [55], the event of cholesterol efflux directly or indirectly alters the membrane fluidity and permeability, leads to increased intracellular calcium concentration, which may induce capacitation-like changes in frozen-thawed buffalo spermatozoa. The cholesterol content in spermatozoa and seminal plasma of water buffalo is 22.2% and 24.7% of total neutral lipids while these ratios in bull are 23.3% and 18.8%; respectively [5]. …”

Section: Biological Roles Of Lipid and Polyunsaturated Fatty Acids Inmentioning

confidence: 99%

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Polyunsaturated Fatty Acids in Male Ruminant Reproduction — A Review

Tran¹,

Malla

Kumar

et al. 2016

Asian-Australas J Anim Sci

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Fatty acids such as n-3 and n-6 polyunsaturated fatty acids (PUFA) are critical nutrients, used to improve male reproductive performance through modification of fatty acid profile and maintenance of sperm membrane integrity, especially under cold shock or cryopreservation condition. Also, PUFA provide the precursors for prostaglandin synthesis and can modulate the expression patterns of many key enzymes involved in both prostaglandin and steroid metabolism. Many studies carried out on diets supplemented with PUFA have demonstrated their capability to sustain sperm motility, viability and fertility during chilling and freezing as well as improving testis development and spermatogenesis in a variety of livestock species. In addition to the type and quantity of dietary fatty acids, ways of addition of PUFA to diet or semen extender is very crucial as it has different effects on semen quality in male ruminants. Limitation of PUFA added to ruminant ration is due to biohydrogenation by rumen microorganisms, which causes conversion of unsaturated fatty acids to saturated fatty acids, leading to loss of PUFA quantity. Thus, many strategies for protecting PUFA from biohydrogenation in rumen have been developed over the years. This paper reviews four aspects of PUFA in light of previous research including rumen metabolism, biological roles, influence on reproduction, and strategies to use in male ruminants.

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“…Arachidic acid is a saturated form of arachidonic acid that is naturally present in buffalo semen (Jain and Anand ). It was hypothesized that supplementation of a saturated fatty acid, arachidic acid, in extender may improve the post‐thaw sperm quality of Nili‐Ravi buffalo bull.…”

Section: Introductionmentioning

confidence: 99%

Arachidic Acid in Extender Improves Post‐thaw Parameters of Cryopreserved Nili‐Ravi Buffalo Bull Semen

Ejaz

Ansari

Rakha

et al. 2013

Reprod Domestic Animals

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Cryopreservation process reduces lipids and phospholipids from buffalo bull spermatozoa. It was therefore hypothesized that supplementation of fatty acid to extender may improve the post-thaw quality of buffalo semen. The objective was to evaluate the effect of arachidic acid supplementation in extender on post-thaw quality of buffalo bull (Bubalus bubalis) spermatozoa. Semen was collected from three adult Nili-Ravi buffalo bulls of similar age group with artificial vagina (42°C) for 3 weeks (replicate). Qualified semen ejaculates (n = 18) were split into four aliquots and diluted in tris-citric acid extender containing 0.0 (control), 5.0, 10.0 and 20.0 ng/ml at 37°C having approximately 50 × 10(6) spermatozoa/ml. Diluted semen was cooled to 4°C in 2 h and equilibrated for 4 h at 4°C. Cooled semen was filled in 0.5-ml straws at 4°C, kept on liquid nitrogen vapours for 10 min and plunged in liquid nitrogen for storage. Thawing of frozen semen was performed after 24 h at 37°C for 30 s. Sperm progressive motility (%) was improved in a dose-dependent manner by supplementing arachidic acid at 5.0, 10.0 and 20.0 ng/ml compared with control. Structural and functional integrity of sperm plasma membrane (%), number of acrosome-intact live sperm (%) and sperm chromatin integrity (%) were better (p < 0.05) in extender having 5.0 ng/ml of arachidic acid compared with control. At 10.0 ng/ml, these values did not vary (p > 0.05) from those at 5.0 ng/ml. Further improvement in structural and functional integrity of sperm plasma membrane, number of acrosome-intact live sperm and chromatin integrity was observed at 20.0 ng/ml of arachidic acid in extender. In conclusion, arachidic acid supplementation in extender improved the post-thaw quality parameters of cryopreserved Nili-Ravi buffalo bull spermatozoa. Among the arachidic acid concentrations studied, maximum improvement in post-thaw semen quality parameters was observed at 20.0 ng/ml.

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Fatty acids and fatty aldehydes of buffalo seminal plasma and sperm lipid

Cited by 32 publications

References 18 publications

Phospholipase and lysophospholipase activities of goat spermatozoa in transit from the caput to the cauda epididymidis

Phospholipase and lysophospholipase activities of goat spermatozoa in transit from the caput to the cauda epididymidis

Polyunsaturated Fatty Acids in Male Ruminant Reproduction — A Review

Arachidic Acid in Extender Improves Post‐thaw Parameters of Cryopreserved Nili‐Ravi Buffalo Bull Semen

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