Fatty acid uptake by cultured human keratinocytes grown in medium deficient in or supplemented with essential fatty acids

Schürer, Nanna; Rippke, Frank; Vogelsang, Kathrin; Schliep, Viola; Ruzicka, Thomas

doi:10.1007/s004030050382

Cited by 15 publications

(7 citation statements)

References 23 publications

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“…Protein expression and healthy skin ultrastructure were not altered by lipid supplementation in growth medium, but an increase in the number of epidermal layers in treated samples was observed, probably due to an increase in the viability. This hypothesis agrees with previous studies evidencing fatty acid supplementation in culture media improves cell growth and promotes epithelial morphogenesis in SEs [ 28 , 34 ].…”

Section: Discussionsupporting

confidence: 94%

Effects of Essential Oils and Polyunsaturated Fatty Acids on Canine Skin Equivalents: Skin Lipid Assessment and Morphological Evaluation

Cerrato¹,

Ramió‐Lluch²,

Fondevila

et al. 2013

Journal of Veterinary Medicine

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A canine skin equivalent model has been validated for the assessment of a topical formulation effects. Skin equivalents were developed from freshly isolated cutaneous canine fibroblasts and keratinocytes, after enzymatic digestion of skin samples (n = 8) from different breeds. Fibroblasts were embedded into a collagen type I matrix, and keratinocytes were seeded onto its surface at air-liquid interface. Skin equivalents were supplemented with essential oils and polyunsaturated fatty acid formulation or with vehicle. Skin equivalents were histopathologically and ultrastructurally studied, and the three main lipid groups (free fatty acids, cholesterol, and ceramides) were analyzed. Results showed that the culture method developed resulted in significant improvements in cell retrieval and confluence. Treated samples presented a thicker epidermis with increased number of viable cell layers, a denser and compact stratum corneum, and a more continuous basal membrane. Regarding lipid profile, treated skin equivalents showed a significant increase in ceramide content (51.7 ± 1.3) when compared to untreated (41.6 ± 1.4) samples. Ultrastructural study evidenced a compact and well-organized stratum corneum in both treated and control skin equivalents. In conclusion, cell viability and ceramides increase, after lipid supplementation, are especially relevant for the treatment of skin barrier disruptions occurring in canine atopic dermatitis.

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Section: Discussionsupporting

confidence: 94%

Effects of Essential Oils and Polyunsaturated Fatty Acids on Canine Skin Equivalents: Skin Lipid Assessment and Morphological Evaluation

Cerrato¹,

Ramió‐Lluch²,

Fondevila

et al. 2013

Journal of Veterinary Medicine

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“…Reconstruct culture medium was supplemented with linoleic acid (LA) (7 × 10 −5 M) (L1012, Sigma‐Aldrich, St Quentin Fallavier, France) and/or arachidonic acid (AA) (3 × 10 −5 M) (A3555, Sigma‐Aldrich), which corresponds to the physiological human serum concentrations (27). No trace of tocopherol was detected in LA, AA or human serum albumin.…”

Section: Methodsmentioning

confidence: 99%

Polyunsaturated fatty acids partially reproduce the role of melanocytes in the epidermal melanin unit

Cario‐André

Briganti

Picardo

et al. 2005

Experimental Dermatology

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The incidence rate of melanoma is higher in fair-skinned than in dark-skinned individuals. In negroid skin there is more eumelanin which is present in all skin layers and fewer polyunsaturated fatty acids (PUFA) than in caucasoid skin. The western diet, which is rich in omega-6 polyunsaturated fatty acids, is associated with more proneness to cancer including cutaneous melanoma. To study the respective influence of omega-6 PUFA and low phototype melanocytes on redox status -basal and following UV irradiation-, we used epidermal reconstructs. The addition of polyunsaturated fatty acids as well as the presence of low phototype melanocytes affected basal status similarly except for catalase activity, which decreased significantly in polyunsaturated fatty acid-supplemented reconstructs. Following UV, polyunsaturated fatty acids and low phototype melanocytes increased lipid and protein oxidative damage without affecting direct DNA damage. However, polyunsaturated fatty acids increased epidermal apoptosis whereas low phototype melanocytes decreased it. Since our data suggest that an omega-6 PUFA rich-diet may increase oxidative damage in melanocytes without inducing apoptosis, the long-term net outcome could be cumulated mutations and an increased risk of skin cancer, especially melanoma.

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“…This is likely to ensure the sufficient availability of linoleic acid for barrier lipid synthesis in the epidermis. Studies with cultured human keratinocytes also show that the uptake of linoleic acid but not that of oleic acid by cells is influenced by extracellular fatty acid composition, and that supplementation of culture medium with essential fatty acids results in cells that better mimic keratinocytes in vivo [21]. …”

Section: Functions Of Fatty Acids In Epidermismentioning

confidence: 99%

Fatty acid transporters in skin development, function and disease

Lin¹,

Khnykin²

2014

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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Fatty acids in the epidermis can be incorporated into complex lipids or exist in a free form, and they are crucial to proper functions of the epidermis and its appendages, such as sebaceous glands. Epidermal fatty acids can be synthesized de novo by keratinocytes or taken up from extracutaneous sources in a process that likely involves protein transporters. Several proteins that are expressed in the epidermis have been proposed to facilitate the uptake of long-chain fatty acids (LCFA) in mammalian cells, including fatty acid translocase/CD36, fatty acid binding protein, and fatty acid transport protein (FATP)/very long-chain acyl-CoA synthetase. In this review, we will discuss the mechanisms by which these candidate transporters facilitate the uptake of fatty acids. We will then discuss the clinical implications of defects in these transporters and relevant animal models, including the FATP4 animal models and ichthyosis prematurity syndrome, a congenital ichthyosis caused by FATP4 deficiency. This article is part of a Special Issue entitled The Important Role of Lipids in the Epidermis and their Role in the Formation and Maintenance of the Cutaneous Barrier.

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Fatty acid uptake by cultured human keratinocytes grown in medium deficient in or supplemented with essential fatty acids

Cited by 15 publications

References 23 publications

Effects of Essential Oils and Polyunsaturated Fatty Acids on Canine Skin Equivalents: Skin Lipid Assessment and Morphological Evaluation

Effects of Essential Oils and Polyunsaturated Fatty Acids on Canine Skin Equivalents: Skin Lipid Assessment and Morphological Evaluation

Polyunsaturated fatty acids partially reproduce the role of melanocytes in the epidermal melanin unit

Fatty acid transporters in skin development, function and disease

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