Fatty acid transport protein-1 mRNA expression in skeletal muscle and in adipose tissue in humans

Abstract

Fatty acid transporter protein (FATP)-1 mRNA expression was investigated in skeletal muscle and in subcutaneous abdominal adipose tissue of 17 healthy lean, 13 nondiabetic obese, and 16 obese type 2 diabetic subjects. In muscle, FATP-1 mRNA levels were higher in lean women than in lean men (2.2 +/- 0.1 vs. 0.6 +/- 0.2 amol/microg total RNA, P < 0.01). FATP-1 mRNA expression was decreased in skeletal muscle in obese women both in nondiabetic and in type 2 diabetic patients (P < 0.02 vs. lean women in both groups), and in all women there was a negative correlation with basal FATP-1 mRNA level and body mass index (r = -0.74, P < 0.02). In men, FATP-1 mRNA was expressed at similar levels in the three groups both in skeletal muscle (0.6 +/- 0.2, 0.6 +/- 0.2, and 0.8 +/- 0.2 amol/microg total RNA in lean, obese, and type 2 diabetic male subjects) and in adipose tissue (0.9 +/- 0.2 amol/microg total RNA in the 3 groups). Insulin infusion (3 h) reduced FATP-1 mRNA levels in muscle in lean women but not in lean men. Insulin did not affect FATP-1 mRNA expression in skeletal muscle in obese nondiabetic or in type 2 diabetic subjects nor in subcutaneous adipose tissue in any of the three groups. These data show a gender-related difference in the expression of the fatty acid transporter FATP-1 in skeletal muscle of lean individuals and suggest that changes in FATP-1 expression may not contribute to a large extent to the alterations in fatty acid uptake in obesity and/or type 2 diabetes.

Expression of key genes of fatty acid oxidation, including adiponectin receptors, in skeletal muscle of Type 2 diabetic patients

“…Figure 2 shows the mRNA abundance of seven genes involved in long-chain fatty acyl CoA (LCFA) trans-membrane transport, cytosolic activation or entry into the mitochondria. The expression of fatty acid transporter protein-1 has been studied previously and was found not to differ in skeletal muscle of control and Type 2 diabetic subjects [23]. Here, we found no difference in the mRNA expression levels of the other potential membrane carriers (FAT/CD36 and FABPpm) and of FABP3, the major isoform of cytosolic fatty acid binding protein expressed in skeletal muscle.…”

Expression of fatty-acid-handling proteins in human adipose tissue in relation to obesity and insulin resistance

“…Moreover, lipodystrophy (which is strongly related to insulin resistance) in HIV-positive patients undergoing highly active antiretroviral therapy was associated with reduced adipose tissue ACS1 expression [23]. In addition, FATP1 mRNA levels in skeletal muscle were negatively correlated with BMI in middle-aged women (but not in men), whereas no such correlation was observed for adipose tissue FATP1 expression, which was analysed in men only [24]. To our knowledge, no studies have shown that FATP4 expression is related to measures of human obesity or insulin resistance.…”

Correlation Analysis of Relative Expression of Apob, Adfp and Fatp1 with Lipid Metabolism in Daweishan Mini Chickens

“…Fatty acid transport protein 1 (FATP1) is a member of the fatty acid transport protein family (FATPs). It is an integrated transmembrane protein involved in the transmembrane transport of fatty acids and in fatty acid metabolism, and the gene that expresses FATP1 is one of the key genes affecting body fat content of the body (Binnert et al, 2000). Because FATP1 can indirectly influence lipid profile and deposition by regulating fatty acid uptake and metabolism in the skeletal muscle, the determination of its expression may be important for the genetic improvement of meat traits (Poh et al, 2004).…”