Fatty acid transfer between serum albumins and shungite carbon nanoparticles and its effect on protein aggregation and association

Goryunov, A. S.; Rozhkov, S. S.; Rozhkova, N. N.

doi:10.1007/s00249-019-01414-y

Cited by 6 publications

(8 citation statements)

References 51 publications

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“…This means that the rGO electrode can extract FAs from albumin without hindrance from the FA/Alb ratios of 6 down to ~1. However, when FA/Alb < 1, competition between albumin and the electrode surface takes place, thus leading to slowing down the extraction kinetics significantly, which is in agreement with a previous study [28]. Interestingly, the LOQ value = 1.06 calculated in our study coincides with the upper limit of the FA/Alb ratio in healthy subjects (that is ≤1).…”

Section: Discussionsupporting

confidence: 92%

Impedimetric Detection of Albumin-Bound Fatty Acids Using Graphene Oxide Electrode

2021

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The fatty acid/albumin (FA/Alb) molar ratio is ≤1 in healthy subjects; this ratio can reach 3–4 in patients with acute myocardial ischemia. We describe the spontaneous desorption–adsorption kinetics of FAs from albumin to a graphene electrode at neutral pH. Albumin-depleted human serum was prepared via ultrafiltration and then mixed with defatted human albumin and sodium oleate at different FA/Alb molar ratios, at a final albumin concentration of 0.6 mM. A commercially available screen-printed graphene oxide (GO)-modified carbon electrode was used for the electrochemical experiments. Frequency-ranged Faradaic electrochemical impedance spectroscopy (EIS) and a single-frequency non-Faradaic impedance measure (chronoimpedance) were used to derive the desorption–adsorption kinetics. The surface of the GO electrode was finally evaluated with the aid of X-ray photoelectron spectroscopy (XPS). With the chronoimpedance experiment, the measured impedance increased accordingly to the FA/Alb ratios. The frequency-ranged EIS showed good linearity between the impedance and the FA/Alb ratio, with a limit of quantification value of 1.06. XPS surface analysis revealed that the FA was adsorbed onto the electrode, with the amount of the adsorbed FA proportional to the FA/Alb ratio. The electrochemical method applied on this peculiar desorption–adsorption kinetics of FAs has the ability to differentiate serum having excess FAs.

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Section: Discussionsupporting

confidence: 92%

Impedimetric Detection of Albumin-Bound Fatty Acids Using Graphene Oxide Electrode

2021

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“…For the first time, the interaction of FA with ShC NPs was discovered by the EPR spin probe method [ 50 , 51 ], where the paramagnetic molecule 5-DOXIL stearic acid (5DSA) was used as the reporter group. Typically, the spin probe 5DSA, initially dissolved in chloroform, precipitates on a glass surface as a film after evaporation of the solvent.…”

Section: Resultsmentioning

confidence: 99%

“…Typically, the spin probe 5DSA, initially dissolved in chloroform, precipitates on a glass surface as a film after evaporation of the solvent. If a drop of dispersion of ShC NPs was applied to the film with 5DSA, then a part of the 5DSA from the film was adsorbed on the ShC [ 50 , 51 ]. As a result, three narrow equidistant spectral lines of the EPR spectrum of 5DSA with different amplitudes were observed in the drop of the ShC dispersion.…”

Section: Resultsmentioning

confidence: 99%

Molecular Serum Albumin Unmask Nanobio Properties of Molecular Graphenes in Shungite Carbon Nanoparticles

Rozhkov,

Goryunov,

Rozhkova

2024

IJMS

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Serum albumin is a popular macromolecule for studying the effect of proteins on the colloidal stability of nanoparticle (NP) dispersions, as well as the protein–nanoparticle interaction and protein corona formation. In this work, we analyze the specific conformation-dependent phase, redox, and fatty acid delivery properties of bovine albumin in the presence of shungite carbon (ShC) molecular graphenes stabilized in aqueous dispersions in the form of NPs in order to reveal the features of NP bioactivity. The formation of NP complexes with proteins (protein corona around NP) affects the transport properties of albumin for the delivery of fatty acids. Being acceptors of electrons and ligands, ShC NPs are capable of exhibiting both their own biological activity and significantly affecting conformational and phase transformations in protein systems.

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“…Using differential scanning microcalorimetry, we found [27] that the affinity of fatty acid-free BSA faf for ShC is higher than for each other, i.e., the BSA faf state is shifted towards a less-associated state in the presence of ShC. Due to the presence of protein monomers, a partial stabilization of the network of hydrogen bonds of water occurs, which is noticeable in Figure 5 by a decrease in the parameter d to 1.3 ± 0.1 in the protein concentration range of 0.1-0.5 mg/mL.…”

Section: Resultsmentioning

confidence: 99%

“…The natural origin of shungite carbon, its structural properties, and its physicochemical features of the main basic elements of ShC NPs with the properties of reduced graphene oxide were described earlier [20][21][22][23][24][25]. The study of structuraldynamic, hydrodynamic, thermodynamic, and redox properties of ShC NPs (and, to a lesser extent, Qz NPs) bio-nanoconjugates with albumin and its associates was carried out earlier by the methods of gel filtration chromatography, differential scanning calorimetry (DSC), electron paramagnetic resonance (EPR) of spin labels and spin probes, dynamic light scattering (DLS), and Raman scattering (RS) [26,27]. The role of ShC NPs in the redistribution of fatty acids (FA) between protein fractions has been established.…”

Section: Introductionmentioning

confidence: 99%

The Role of Water Hydrogen Bonds in the Formation of Associates and Condensates in Dispersions of Serum Albumin with Shungite Carbon and Quartz Nanoparticles

et al. 2023

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The role of the network of water hydrogen bonds in the regulation of the intermolecular interaction’s responsible for colloidal stability of dispersions has been studiedin order to search for general patterns of interaction between water, nanoparticles, and bio-macromolecules. Raman spectroscopy for mixed dispersions of bovine serum albumin (SA), shungite carbon nanoparticles (ShC NPs), and quartz nanoparticles (quartz NPs) was performed within the wave number range 3200–3600 cm−1. The main spectral lines in this range are caused by the OH stretch vibrations of water molecules. We analyzed the state of the water hydrogen bonding network for dispersions of varied ratios of both fatty acid-containing and fatty acid-free SA macromolecules, ShC NPs, and silica NPs in the range 0.01–10 mg/mL.We used dynamic light scattering to control the sizes of the protein associates and protein associates with ShC NPs and quartz NPs. The strength of the hydrogen bonds in water depends essentially non-linearly, but in a qualitatively similar way, on the concentrations of the dispersion components. The initial strengthening of the bonds is followed by their loosening with a further increase in the concentration of the components. This is accompanied by the association of the dispersion components. We estimate the thickness of the protein corona layer as 20–25 nm for ShC NPs and 28–33 nm for quartz NPs, depending on the SA concentration. Colloidal stability of the aqueous dispersion is determined almost completely by an association of the protein with NPs. In contrast, colloidal stability of a pure protein solution is regulated by the formation of protein clusters of two main types and sizes. The association effects of SA with ShC NPs are evident in microscopic images of condensate films. The structures differ significantly for native and fatty acid-free SA in shape and size.

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Fatty acid transfer between serum albumins and shungite carbon nanoparticles and its effect on protein aggregation and association

Cited by 6 publications

References 51 publications

Impedimetric Detection of Albumin-Bound Fatty Acids Using Graphene Oxide Electrode

Impedimetric Detection of Albumin-Bound Fatty Acids Using Graphene Oxide Electrode

Molecular Serum Albumin Unmask Nanobio Properties of Molecular Graphenes in Shungite Carbon Nanoparticles

The Role of Water Hydrogen Bonds in the Formation of Associates and Condensates in Dispersions of Serum Albumin with Shungite Carbon and Quartz Nanoparticles

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