Fatty Acid Synthesis from Glutamate in the Adipose Tissue of Normal Subjects and Obese Patients: An Enzyme Study

Belfiore, Francesco; Iannello, Silvia

doi:10.1006/bmme.1995.1003

Cited by 22 publications

(17 citation statements)

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“…However, the biological significance of this dietary-induced modulation needs to be further assessed in pig adipose tissue. Indeed, this first central enzyme in glutamate metabolism could also participate in fatty acid synthesis through the backward pathway of the Krebs cycle as shown in human adipose tissue [32,33]. Together, these changes may indicate a reduced level of oxidative stress inside the adipocytes of sucking piglets fed HP formula or could simply reflect the inflammatory status of adipose tissue.…”

Section: Discussionmentioning

confidence: 98%

Adipose tissue proteomes of intrauterine growth-restricted piglets artificially reared on a high-protein neonatal formula

Sarr

Louveau

Huërou‐Luron

et al. 2012

The Journal of Nutritional Biochemistry

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Section: Discussionmentioning

confidence: 98%

Adipose tissue proteomes of intrauterine growth-restricted piglets artificially reared on a high-protein neonatal formula

Sarr

Louveau

Huërou‐Luron

et al. 2012

The Journal of Nutritional Biochemistry

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“…The NAD-dependent mitochondrial IDH has been assumed to play a major role in the oxidative decarboxylation of isocitrate in the tricarboxylic acid cycle [71], however, the exact roles of mitochondrial and cytoplasmic NADP dependent IDHs, which catalyze decarboxylation of isocitrate into a-ketoglutarate with concurrent production of NADPH in the mitochondria and cytosol, respectively, have not been elucidated. There is evidence that the cytosolic enzyme participates in the production of NADPH [72] and in the biosynthesis of fatty acids [73]. Mitochondrial energy metabolism and Krebs cycle activities are developmentally regulated in the life cycle of trypanosomatidae.…”

Section: Discussionmentioning

confidence: 99%

A proteomic approach to identify developmentally regulated proteins in Leishmania infantum

2002

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We used comparative two-dimensional gel electrophoresis (2-DE) and mass spectrometry methodologies to highlight and identify proteins that are differentially expressed in the intracellular stage of the parasite Leishmania donovani infantum, a causative agent of visceral leishmaniasis. During its digenetic life cycle, Leishmania alternates between the alimentary tract of the sandfly vector as an extracellular promastigote and the acidic phagolysosomes of macrophage cells as an intracellular amastigote. Proteins differentially expressed in the intracellular form of the parasite are thought to be important for intracellular survival and pathogenesis. We used narrow pH range strips for isoelectric focusing to resolve soluble proteins of both developmental stages of L. infantum. More than 62 proteins differentially expressed in amastigotes were detected among approximately 2000 protein spots resolved by 2-DE. A quadrupole time-of-flight analysis of few selected protein spots, specifically expressed in the amastigote stage, permitted the identification of two proteins, part of the energetic metabolism pathways, the isocitrate dehydrogenase and the glycolytic enzyme triosephosphate isomerase. The kinetic parameters of these two enzymes were measured in both developmental stages of the parasite and their activity was indeed found to be higher in amastigotes. These findings bring a new insight in our understanding of metabolic and energy requirements of the intracellular form of Leishmania. Comparative analysis of the proteome of both developmental stages of the protozoan parasite Leishmania should permit the identification of protein candidates for the development of vaccines and new drugs.

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“…This result suggests that the contribution of IDH1 to NADPH production may be significant. There are growing indications that IDH1 participates in cytosolic NADPH production and in fatty acid biosynthesis (32,33). In support of this idea, IDH1 was also identified as a major source of cytosolic NADPH needed for the regeneration of reduced glutathione, critically important in cellular defense against oxidative damage (32,34).…”

mentioning

confidence: 91%

IDH1 gene transcription is sterol regulated and activated by SREBP-1a and SREBP-2 in human hepatoma HepG2 cells

Shechter

Dai

Huo

et al. 2003

Journal of Lipid Research

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Fatty Acid Synthesis from Glutamate in the Adipose Tissue of Normal Subjects and Obese Patients: An Enzyme Study

Cited by 22 publications

References 0 publications

Adipose tissue proteomes of intrauterine growth-restricted piglets artificially reared on a high-protein neonatal formula

Adipose tissue proteomes of intrauterine growth-restricted piglets artificially reared on a high-protein neonatal formula

A proteomic approach to identify developmentally regulated proteins in Leishmania infantum

IDH1 gene transcription is sterol regulated and activated by SREBP-1a and SREBP-2 in human hepatoma HepG2 cells

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