Fatty acid profiles of PUFA producing Antarctic bacteria: correlation with RAPD analysis

Abstract

The significance of polyunsaturated fatty acids (PUFA) to humans lies in their biological activity, as precursors for groups of nutritionally important compounds and as essential cellular components. They are also crucial in animals for the proper development of retinal and nervous tissue. In this work, Antarctic sea samples were used to isolate bacterial cultures producing PUFA. Three bacterial strains were isolated from three different samples and they were named as BRI 1, BRI 7 and BRI 28. Percentage of eicosapentaenoic acid (EPA) was 42.7 and 42.6 of total fatty acids in BRI 7 and 28 respectively. Whereas, docosahexaenoic acid (DHA) (1.4%) was detected only in BRI 28. BRI 7 and 28 showed considerable similarities in their fatty acid profiles, hence these three isolates were further used for random amplification of polymorphic DNA (RAPD) analysis using nine random primers to confirm their genetic relatedness. In contrast to fatty acid profiles, maximum polymorphism (58%) was obtained between BRI 7 and 28, while maximum monomorphism (83.63%) was observed between BRI 1 and 7. Our results indicate that, although the conservation of PUFA genes may point to their potential utilization as phylogenetic markers in culture-independent ecological studies, the biosynthetic diversity of bacterial PUFA production has yet to be fully described.

Paradevosia shaoguanensis gen. nov., sp. nov., Isolated from a Coking Wastewater

“…With the ten random primers, strain J5-3 T shared plenty of polymorphic bands but few monomorphic bands with the type strains in genera Devosia and Pelagibacterium. The monomorphisms, the number of monomorphic bands against the total bands, between strain J5-3 T riboflavina DSM 7230 T ), and 26.3 % (P. halotolerans CGMCC 1.7692 T ), which were low enough to distinguish strain J5-3 T from all the four reference strains [11,22]. The results were consistent with the relatively low 16S rRNA and gyrB gene sequence similarities, which indicated that strain J5-3 T was separated from reference strains.…”

“…As a simple, rapid, and efficient molecular marker, RAPD is usually used for germplasm identification and genetic diversity researches [11,19,22,34,38]. After the genomic DNA were extracted from strain J5-3 T and all the four reference strains [18], RAPD analysis was carried out in 50 lL reaction mixture containing 5 lL 109 PCR buffer (Takara), 4 lL dNTP mixture (Takara), 1 U Taq polymerase (Takara), 20 lM primer (Supplementary Table 4), and 20 ng template DNA.…”

Characterization of novel extracellular protease produced by marine bacterial isolate from the Indian Ocean

“…We have earlier reported halotolerant organisms from wall scrapings of historical building in India (13) and also from some food samples (14) with an ability to produce industrially useful enzymes. Recently we have characterized some marine isolates from the Antarctic Ocean as well as from the Indian Ocean with respect to their fatty acid profiles (15,32). Comparatively few reports are available on the saline-tolerant proteases from microbial sources.…”

The microbial diversity of Polar environments is a fertile ground for bioprospecting

“…Early studies indicated the n −3 PUFA accumulation was a characteristic of deep-sea bacteria (DeLong and Yayanos, 1986;Wirsen et al, 1987), suggesting that it was a barophilic as much as a lowtemperature adaptation. The capacity for n −3 PUFA production has, however, later been found to be equally widespread in nonbarophilic, cold-adapted bacteria (Henderson et al, 1993;Yano et al, 1997;Cho and Mo, 1999;Nichols, 2003;Frolova et al, 2011), including Arctic and Antarctic strains (Nichols et al, 1993;Jøstensen and Landfald, 1997;Bowman et al, 1998;Jadhav et al, 2010). Mutant studies have unequivocally associated PUFA production in bacteria with cold adaptation, as growth is impaired at low, but not elevated temperatures by knocking out this trait in e.g.…”