Fatty acid profile of maternal and fetal erythrocytes and placental expression of fatty acid transport proteins in normal and intrauterine growth restriction pregnancies

Assumpção, Roberto Pereira; Mucci, Daniela de Barros; Fonseca, Fernanda Carrilho Pinto da; Marcondes, Henrique; Sardinha, Fátima Lúcia de Carvalho; Citelli, Marta; Carmo, Maria G. Tavares do

doi:10.1016/j.plefa.2017.08.011

Cited by 22 publications

(19 citation statements)

References 44 publications

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“…We hypothesise a preferential transfer of this polyunsaturated fatty acid through the placenta due to the increased needs of the foetus. The possible mechanism could be through an up-regulation of mRNA expression in placental fatty acid transporters as a compensatory mechanism in SGA foetuses [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

Association between Maternal and Foetal Erythrocyte Fatty Acid Profiles and Birth Weight

et al. 2018

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Regular foetal development is crucial for assuring good health status in the offspring. The quality and quantity of maternal dietary fatty acids (FAs) can affect growth. The study aimed to: (1) investigate the association of maternal/foetal lipid profiles with birth weight (BW); and (2) compare these profiles in small, appropriate, and large for gestational age (SGA, AGA, and LGA) infants. FAs were measured in erythrocyte membranes using gas chromatography analysis in 607 mother–infant pairs (316 males, 52.1%). In the quantile regression, a significant association between BW and levels of maternal linoleic acid (LA; C18:2, n-6; coefficient: 18.66; p = 0.010), arachidonic acid (AA; C20:4, n-6; coefficient: 11.35; p = 0.007), docosahexaenoic acid (DHA; C22:6, n-3; coefficient: 29.73; p = 0.007), polyunsaturated FAs (coefficient: 8.55; p = 0.001), foetal DHA (coefficient: −22.82; p = 0.037), and saturated FAs (coefficient: −65.41; p = 0.002) was found. Myristic (C14:0) and pentadecanoic acids (C15:0), both maternal (p = 0.000; p = 0.017) and foetal (p = 0.009; p = 0.002), and maternal erucic acid (C22:1, n-9; p = 0.026) were found at higher levels in SGA infants as compared to AGA ones. Conversely, maternal LA, AA, and omega 6 FAs levels were higher in AGA infants (p = 0.037; p = 0.003; p = 0.026, respectively). Maternal and foetal polyunsaturated and omega 6 FAs levels are positively related to BW, while a lipid profile rich in saturated FAs and erucic acid may influence the risk of SGA.

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Section: Discussionmentioning

confidence: 99%

Association between Maternal and Foetal Erythrocyte Fatty Acid Profiles and Birth Weight

et al. 2018

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“…A recent publication described the same FA in erythrocytes from adult subjects although in different percentages (Brenna et al, ). In relation to umbilical erythrocytes, a study in Brazilian healthy newborns reported that these FA represent 88% of the FA (Assumpção et al, ). Another study performed in Polish newborns found lower EPA (0.3%), 22:4n‐6 (1.7%), and DPA n‐3 (0.4%) and higher LNA (6.5%), ALA (0.3%), 20:3n‐6 (1.9%), ARA (10.4%), and DHA (3.6%) compared to the current results (Pankiewicz et al, ).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Stability of Fatty Acids in Erythrocytes from Human Umbilical Cord

Jaramillo

Garmendia

Muñóz

et al. 2020

Lipids

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The interest in the amount of polyunsaturated fatty acids (PUFA) in the umbilical cord blood (UCB) is increasing, but the stability of erythrocyte PUFA in these samples during storage and washing of the erythrocytes has not been directly evaluated. The purpose of this study was to analyze the effect of the lapse of time on the fatty acid (FA) content from UCB sample collection and maintained at 4 C (0-12 h) until erythrocyte separation and washing. Palmitic acid (16:0), stearic acid (18:0), 18:1n-7/n-9, linoleic acid (18:2n-6), arachidonic acid (20:4n-6), 22:4n-6, eicosapentaenoic acid (20:5n-3), docosapentaenoic acid (22:5n-3), and docosahexaenoic acid (22:6n-3) together accounted for 87% of the FA profile in the umbilical vein erythrocytes. No difference was observed in the concentration of any of the FA studied, nor in the sum of saturated fatty acids (SFA), PUFA, or LC-PUFA in umbilical erythrocytes obtained at delivery and stored up to 12 h before the separation of erythrocytes. However, if a washing step was included in the processing of the erythrocytes, a decrease in the concentration of 16:0, 18:0, 18:3n-3, 20:4n-6, 22:4n-6, total SFA, PUFA, LC-PUFA, and n-6 LC-PUFA was evidenced, compared to unwashed erythrocytes. The FA concentration in umbilical cord erythrocytes did not change between samples stored from 0 to 12 h until erythrocyte separation. Erythrocyte washing before storage decreased the concentration of significant individual and total SFA, PUFA, and LC-PUFA. These results should be considered when planning the collection of UCB samples for the study of fatty acid concentration due to the nonscheduled timing of deliveries.

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“…Fatty acids can be transferred across the placenta by simple diffusion or via mediation by FATPs (Chassen, Ferchaud‐Roucher, Gupta, Jansson, & Powell, ). The impairment of FATP functions is linked to abnormal fetal development (Assumpcao et al, ; Chassen et al, ; Ye et al, ). In the present study, the expression of FATP4, which is the predominant FATP member in pig placenta, was downregulated in SCNT placentas relative to that in AI placentas.…”

Section: Discussionmentioning

confidence: 99%

“…Developing mammalian fetuses show a limited capacity to synthesize some saturated and monounsaturated fatty acids and are unable to produce de novo polyunsaturated fatty acids. Therefore, the considerable bulk of fatty acids must be provided by the maternal circulation through the placenta to achieve proper fetal The impairment of FATP functions is linked to abnormal fetal development (Assumpcao et al, 2017;Chassen et al, 2018;Ye et al, 2017). In the present study, the expression of FATP4, which is the predominant FATP member in pig placenta, was downregulated in SCNT placentas relative to that in AI placentas.…”

Section: Discussionmentioning

confidence: 99%

Cloned pig fetuses exhibit fatty acid deficiency from impaired placental transport

Zhou

et al. 2019

Molecular Reproduction Devel

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Cloned pig fetuses produced by somatic cell nuclear transfer show a high incidence of erroneous development in the uteri of surrogate mothers. The mechanisms underlying the abnormal intrauterine development of cloned pig fetuses are poorly understood. This study aimed to explore the potential causes of the aberrant development of cloned pig fetuses. The levels of numerous fatty acids in allantoic ﬂuid and muscle tissue were lower in cloned pig fetuses than in artificial insemination‐generated pig fetuses, thereby suggesting that cloned pig fetuses underwent fatty acid deficiency. Cloned pig fetuses also displayed trophoblast hypoplasia and a reduced expression of placental fatty acid transport protein 4 (FATP4), which is the predominant FATP family member expressed in porcine placentas. This result suggested that the placental fatty acid transport functions were impaired in cloned pig fetuses, possibly causing fatty acid deficiency in cloned pig fetuses. The present study provides useful information in elucidating the mechanisms underlying the abnormal development of cloned pig fetuses.

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Fatty acid profile of maternal and fetal erythrocytes and placental expression of fatty acid transport proteins in normal and intrauterine growth restriction pregnancies

Cited by 22 publications

References 44 publications

Association between Maternal and Foetal Erythrocyte Fatty Acid Profiles and Birth Weight

Association between Maternal and Foetal Erythrocyte Fatty Acid Profiles and Birth Weight

Evaluation of the Stability of Fatty Acids in Erythrocytes from Human Umbilical Cord

Cloned pig fetuses exhibit fatty acid deficiency from impaired placental transport

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