Fatty Acid Profile and<i>in Vitro</i>Biological Activities of<i>Allophylus africanus</i>(P. Beauv)

Balogun, Olaoye Solomon; Oladosu, I. A.; Liu, Zhiqiang

doi:10.1080/10496475.2016.1193587

Cited by 8 publications

(5 citation statements)

References 22 publications

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“…While the leaves have been characterized on their content in terpenoids [ 12 ], fatty acids [ 16 ], and phenolic constituents [ 18 ], so far, only apigenin di -C- glycosides and mono -C- glycosides -O- glycosylated have been identified by us in an aqueous extract obtained from A. africanus stem bark [ 18 ]. Differently from our previous report [ 18 ], we have now characterized an hydroethanol extract obtained from stem bark samples of A. africanus , also collected in Guinea-Bissau, but in a different location (Formosa Island, in October 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Ethnomedicinal reports covering bark medicinal uses are less common, but it is worth mentioning its use as a vermicide, antimalarial, antiarthritic, and antidiarrheal agent [ 9 , 13 , 14 , 15 ]. Studies on the chemical characterization of A. africanus are basically limited to the fatty acid profiling of the leaves and flowers and their essential oils [ 16 , 17 ], isolation of the terpenes alloeudesmenol, hanocokinoside, allotaraxerolide, and alloaminoacetaldehyde also being described [ 12 ]. Characterization of the flavonoid profile of aqueous extracts obtained from the plant enabled the detection of 30 flavones, derived from apigenin and luteolin, on the leaves, apigenin derivatives being identified on the stem bark [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

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Allophylus africanus Stem Bark Extract Modulates the Mitochondrial Apoptotic Pathway in Human Stomach Cancer Cells

Ribeiro

Ferreres

Oliveira

et al. 2023

Life

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The present work aimed to detail the mechanisms elicited by Allophylus africanus P. Beauv. stem bark extract in human stomach cancer cells and to identify the bioactives underlying the cytotoxicity. MTT reduction and LDH leakage assays allowed characterizing the cytotoxic effects in AGS cells, which were further detailed by morphological analysis using phalloidin and Hoechst 33258. Proapoptotic mechanisms were elucidated through a mitochondrial membrane potential assay and by assessing the impact upon the activity of caspase-9 and -3. The extract displayed selective cytotoxicity against AGS cells. The absence of plasma membrane permeabilization, along with apoptotic body formation, suggested that pro-apoptotic effects triggered cell death. Intrinsic apoptosis pathway activation was verified, as mitochondrial membrane potential decrease and activation of caspase-9 and -3 were observed. HPLC-DAD profiling enabled the identification of two apigenin-di-C-glycosides, vicenin-2 (1) and apigenin-6-C-hexoside-8-C-pentoside (3), as well as three mono-C-glycosides-O-glycosylated derivatives, apigenin-7-O-hexoside-8-C-hexoside (2), apigenin-8-C-(2-rhamnosyl)hexoside (4) and apigenin-6-C-(2-rhamnosyl)hexoside (5). Isovitexin-2″-O-rhamnoside (5) is the main constituent, accounting for nearly 40% of the total quantifiable flavonoid content. Our results allowed us to establish the relationship between the presence of vicenin-2 and other apigenin derivatives with the contribution to the cytotoxic effects on the presented AGS cells. Our findings attest the anticancer potential of A. africanus stem bark against gastric adenocarcinoma, calling for studies to develop herbal-based products and/or the use of apigenin derivatives in chemotherapeutic drug development.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Allophylus africanus Stem Bark Extract Modulates the Mitochondrial Apoptotic Pathway in Human Stomach Cancer Cells

Ribeiro

Ferreres

Oliveira

et al. 2023

Life

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“…It is widely spread in tropical and subtropical regions of the America, Africa, Asia and Indian archipelago (Chavan and Gaikwad, 2016). Scientifically, it is reported to have strong antimalarial, antibacterial and antioxidant activities (Sofidiya et al, 2012;Balogun et al, 2016). One of the species from the same genus, Allophylus cobbe, was confirmed to have anticancer activity against human prostate cancer cell lines (Ghagane et al, 2017).…”

Section: Introductionmentioning

confidence: 98%

“…It has been reported to be effective against cervical, skin and pancreatic cancers (Graham et al, 2000;Okoye et al, 2014). A. africanus belongs to the family Sapindaceae (Balogun et al, 2016). It is widely spread in tropical and subtropical regions of the America, Africa, Asia and Indian archipelago (Chavan and Gaikwad, 2016).…”

Section: Introductionmentioning

confidence: 99%

In vitro antiproliferative potential of Annona senegalensis Pers. and Allophylus africanus P Beauv. plant extracts against selected cancer cell lines

Biseko¹,

Swai²,

Mbugua³

et al. 2019

J. Med. Plants Res.

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The medicinal plants Annona senegalensis Pers. and Allophylus africanus P Beauv. are used in Tanzania traditional medicine for the treatment of cancer. However, there is no scientific documentation on their therapeutic effectiveness. The present study evaluated antiproliferative potential as an indicator of anticancer activity of A. senegalensis and A. africanus plant species from Tanzania. A. senegalensis and A. africanus were collected from Ugweno village at Kilimanjaro, Tanzania. Different types of extracts were prepared in dichloromethane/methanol (DCM:MeOH), petroleum ether, DCM, ethyl acetate (EtOAc), MeOH and water respectively. Antiproliferative activity against HCC 1396 (breast), HEp-2 (throat) and CT 26 (colon) cancer cell lines was assessed by the MTT cell viability assay. The results of the present study showed that the antiproliferative activity varied between plant extracts and the cancer cell lines. The highest antiproliferative activity was achieved with petroleum ether extract of A. senegalensis against HEp-2 with an IC 50 value of 0.42 ± 0.09 μg/ml. This also depicted the highest selectivity to cancerous cells (SI value 94.19) compared to the other extracts. A. africanus also depicted good antiproliferative activity against HEp-2 with IC 50 values of 1.00 ± 0.41 and 2.37 ± 1.45 μg/ml for DCM:MeOH and petroleum ether extracts, respectively. The findings validate the traditional use of A. senegalensis and A. africanus in the treatment of cancer. Results also support previous studies which demonstrated the effect of extraction solvent used in extraction of bioactive agents from medicinal plants. Further studies involving the isolation of pure antiproliferative compounds against cancer cells from the two plants are recommended to elucidate bioactive molecules.

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“…Allophylus is the largest genus of a family Sapindaceae (Balogun, Oladosu, & Liu, 2016). This genus is widely distributed in tropical and subtropical regions of the America, Africa, Asia, Indian Archipelago and Pacific (Chavan & Gaikwad, 2016).…”

Section: Allophylus Africanusmentioning

confidence: 99%

Evaluation of anti-cancer potential of crude extracts of Annona senegalensis Pers. and Allophylus africanus P Beauv.

Biseko¹

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The medicinal plants Annona senegalensis Pers. and Allophylus africanus P Beav. are traditionally used for the treatment of cancer in Tanzania. However, there is no scientific documentation on their therapeutic effectiveness. To evaluate the anticancer potential of A. senegalensis and A. africanus plant species from Tanzania, stem bark of the two plants were collected from Ugweno village at Kilimanjaro, Tanzania. Pulverized plant materials were soaked in dichloromethane/methanol (DCM:MeOH), petroleum ether (PE), DCM, ethyl acetate (EtOAC), MeOH and water to obtain DCM-MeOH, PE, DCM, EtOAC and MeOH extracts respectively. Anticancer activity against breast (HCC 1396), throat (HEp- 2) and colon (CT 26) cancer cell lines was assessed by the MTT cell viability assay. Results showed that anticancer activity varied between plant extracts and the cancer cell lines. The highest anticancer activity was achieved with A. senegalensis petroleum ether extract against HEp-2. The findings justify traditional use of A. senegalensis and A. africanus in treatment of cancer. This study found petroleum ether extract of A. senegalensis to have high potential for development of an anticancer agent against throat cancer. Further studies involving the isolation of pure anticancer compounds from the two plants are recommended to elucidate bioactive molecules with anticancer activity.

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Fatty Acid Profile andin VitroBiological Activities ofAllophylus africanus(P. Beauv)

Cited by 8 publications

References 22 publications

Allophylus africanus Stem Bark Extract Modulates the Mitochondrial Apoptotic Pathway in Human Stomach Cancer Cells

Allophylus africanus Stem Bark Extract Modulates the Mitochondrial Apoptotic Pathway in Human Stomach Cancer Cells

In vitro antiproliferative potential of Annona senegalensis Pers. and Allophylus africanus P Beauv. plant extracts against selected cancer cell lines

Evaluation of anti-cancer potential of crude extracts of Annona senegalensis Pers. and Allophylus africanus P Beauv.

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