Fatty acid methyl ester analysis of
            <i>Aspergillus fumigatus</i>
            isolated from fruit pulps for biodiesel production using GC-MS spectrometry

Aşçi, Ferruh; AYDIN, Büşra; Akkuş, Gülderen Uysal; Ünal, Arzu; Erdoğmuş, Sevim Feyza; Korcan, Safiye Elif; Jahan, Israt

doi:10.1080/21655979.2020.1739379

Cited by 20 publications

(8 citation statements)

References 29 publications

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“…The finding here that medium-chain saturated capric acid was the dominant FAME in A. fumigatus and the second most abundant in Aspergillus sp. (BW4) contrasts the report from Asci et al (2020) who showed that the FAMEs profile of the six A. fumigatus strains studied were dominated by long-chain fatty acids and suitable for use as biodiesel.…”

Section: Discussioncontrasting

confidence: 61%

FAMEs Profile of Oil Produced by Oleaginous Fungi Isolated from Fermented Beverage Wastewaters and Soil

Nsa¹,

Adeloye²,

Odunsi³

et al. 2020

Nig. J. Biotechnol

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Fungal strains isolated from fermented maize (ogi) (PW) and sorghum-based brewery wastewaters (BW) and two soil isolates were evaluated for oleaginicity. The fungal isolates from the wastewater that had lipid content of at least 20% of their biomass were identified by both culture methods and internal transcribed spacer (ITS) 1-5.8S-ITS2 ribosomal DNA sequencing. The isolates were identified to be Aspergillus fumigatus (PW8), Aspergillus flavus (PW10), Candida tropicalis (PW16) and Aspergillus tubingensis (PW3), Trichosporon luoberi (BW7), Aspergillus sp. (BW4) and Candida tropicalis (BW1; BW3). FAMEs composition was determined for the four strains with the highest lipid content by acid-catalyzed transesterification and analyzed by Gas Chromatography-Flame Ionization Detector (GC-FID). Palmitoleic acid was the dominant fatty acid in M. circinelloides and T. reesei, and the best producers of capric and lauric acids were Aspergillus fumigatus and Aspergillus sp. (BW4), respectively. These fatty acids are beneficial in making cosmetics and pharmaceuticals (antimicrobials and dietary supplements). The analysis of the FAMEs profile in the species indicated low amounts or absence of some key long chain fatty acid (LCFA) constituents of biodiesels. Based on the FAMEs profile of M. circinelloides investigated, this strain could hold promise for use as feedstock for biodiesel with genetic engineering and a tailored lipid production favouring enrichment of LCFA. Keywords: Fungal lipids, wastewater, fatty acid methyl ester, GC-FID

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Section: Discussioncontrasting

confidence: 61%

FAMEs Profile of Oil Produced by Oleaginous Fungi Isolated from Fermented Beverage Wastewaters and Soil

Nsa¹,

Adeloye²,

Odunsi³

et al. 2020

Nig. J. Biotechnol

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“…In the present study, 19 different types of FAMEs in SMB were reported for the first time (Table 2). The presence of long-chain fatty acids of FAME profile are the indications of improved properties of biodiesel, and therefore, indorses high fuel efficiency [60]. While other researchers using the same feedstock reported less number of FAMEs such as Takase et al [17] reported five FAMEs, Ullah et al, [59] reported seven FAMEs, and Fadhil et al [18] reported six FAMEs.…”

Section: Qualitative and Quantitative Analysis Of Smbmentioning

confidence: 98%

Biodiesel Synthesis from Milk Thistle Extract of <em>Silybum marianum</em> (L.) Gaertn. using ZnO Nanoparticles as A Catalyst

Jan¹,

Šurina²,

Al‐Fatesh³

et al. 2022

Preprint

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Biodiesel is considered valuable to reduce dependency on petro-fuels. This work aimed to synthesize biodiesel from Silybum marianum using synthesized ZnO nanoparticles as a catalyst. The synthesized ZnO nanoparticles were examined by scanning electron microscopy and X-ray diffraction for confirmation. The synthesized biodiesel was confirmed by ASTM D-6751, H and C-NMR, GC-MS, and FT-IR spectroscopy. The optimal biodiesel yield of 91% was obtained in the oil-to-methanol ratio of 1:24, 15 mg of catalyst concentration, 60 0C temperature, and 45 minutes of reaction time. Fuel properties were determined according to the ASTM defined methods and found within the defined limits of ASTM D-6751. 1H-NMR and 13C-NMR showed characteristic peaks at 3.667 ppm, 2.000 to 2.060 ppm, 0.858-0.918 ppm, 5.288-5.407 ppm, 24.93-34.22 ppm, 172.71, 173.12, 130.16 ppm, and 128.14 ppm, respectively, which confirm biodiesel synthesis. The FAMEs composition of biodiesel was determined by GC-MS which recognized 19 peaks for different types of FAMEs. FT-IR spectroscopy showed two main peaks, first in the range of 1725-1750 cm-1 and second in the range of 1000-1300 cm-1, which confirmed that the transesterification process was completed successfully. The physicochemical characteristics of Silybum marianum confirm that it is a suitable source to produce biodiesel on industrial scale.

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“…Biodiesel is considered a renewable transportation fuel due to its excellent properties such as biodegradability, nontoxicity, and bio-renewability. Biodiesel can be directly utilized in a compression ignition engine without modification [ 96 , 97 ]. Biodiesel production using a chemical catalyst is an energy-intensive process and also generates wastewater.…”

Section: Polymeric Matrices Immobilized Enzymes For Industrial Bio-ca...mentioning

confidence: 99%

Mechano-chemical and biological energetics of immobilized enzymes onto functionalized polymers and their applications

et al. 2022

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Enzymes of commercial importance, such as lipase, amylase, laccase, phytase, carbonic anhydrase, pectinase, maltase, glucose oxidase etc ., show multifunctional features and have been extensively used in several fields including fine chemicals, environmental, pharmaceutical, cosmetics, energy, food industry, agriculture and nutraceutical etc . The deployment of biocatalyst in harsh industrial conditions has some limitations, such as poor stability. These drawbacks can be overcome by immobilizing the enzyme in order to boost the operational stability, catalytic activity along with facilitating the reuse of biocatalyst. Nowadays, functionalized polymers and composites have gained increasing attention as an innovative material for immobilizing the industrially important enzyme. The different types of polymeric materials and composites are pectin, agarose, cellulose, nanofibers, gelatin, and chitosan. The functionalization of these materials enhances the loading capacity of the enzyme by providing more functional groups to the polymeric material and hence enhancing the enzyme immobilization efficiency. However, appropriate coordination among the functionalized polymeric materials and enzymes of interest plays an important role in producing emerging biocatalysts with improved properties. The optimal coordination at a biological, physical, and chemical level is requisite to develop an industrial biocatalyst. Bio-catalysis has become vital aspect in pharmaceutical and chemical industries for synthesis of value-added chemicals. The present review describes the current advances in enzyme immobilization on functionalized polymers and composites. Furthermore, the applications of immobilized enzymes in various sectors including bioremediation, biosensor and biodiesel are also discussed.

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Fatty acid methyl ester analysis of Aspergillus fumigatus isolated from fruit pulps for biodiesel production using GC-MS spectrometry

Cited by 20 publications

References 29 publications

FAMEs Profile of Oil Produced by Oleaginous Fungi Isolated from Fermented Beverage Wastewaters and Soil

FAMEs Profile of Oil Produced by Oleaginous Fungi Isolated from Fermented Beverage Wastewaters and Soil

Biodiesel Synthesis from Milk Thistle Extract of <em>Silybum marianum</em> (L.) Gaertn. using ZnO Nanoparticles as A Catalyst

Mechano-chemical and biological energetics of immobilized enzymes onto functionalized polymers and their applications

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