Fatty acid metabolism in human preimplantation embryos

Haggarty, Paul; Wood, Maureen J.; Ferguson, Elizabeth M.; Hoad, Gwen; Srikantharajah, Arasaratnam; Milne, Eric; Hamilton, Margaret Elizabeth; Bhattacharya, Siladitya

doi:10.1093/humrep/dei385

Cited by 119 publications

(93 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Also of note was the high percentage of unsaturated FAs, in particular linoleic acid (C18:2n6c), in blastocysts, which was strikingly greater than that in the in vivo derived oocytes. Working with the discarded human pre-implantation embryos, Haggarty et al (2006) observed those that developed beyond the four-cell stage to have a higher relative percentage of unsaturated FAs, particularly linoleic acid, although, unlike sheep blastocysts in the current study, saturated FAs at around 62% TFA remained the largest component. Furthermore, these authors reported that, relative to 13 C palmitic acid, the uptake of added 13 C linoleic acid increased in embryos that developed beyond the eightcell stage.…”

Section: Effects Of N-3 and N-6 Hdls During Embryo Culturementioning

confidence: 47%

Dietary omega-3 and -6 polyunsaturated fatty acids affect the composition and development of sheep granulosa cells, oocytes and embryos

Wonnacott¹,

Kwong²,

Hughes³

et al. 2010

Reproduction

129

104

View full text Add to dashboard Cite

The evidence that omega-3 (n-3) and -6 (n-6) polyunsaturated fatty acids (PUFAs) have differential effects on ovarian function, oocytes and embryo quality is inconsistent. We report on the effects of n-3 versus n-6 PUFA-enriched diets fed to 36 ewes over a 6-week period, prior to ovarian stimulation and follicular aspiration, on ovarian steroidogenic parameters and embryo quality. Follicle number and size were unaltered by diet, but follicular-fluid progesterone concentrations were greater in n-3 PUFA-fed ewes than in n-6 PUFA-fed ewes. The percentage of saturated FAs (mostly stearic acid) was greater in oocytes than in either granulosa cells or plasma, indicating selective uptake and/or de novo synthesis of saturated FAs at the expense of PUFAs by oocytes. High-density lipoproteins (HDLs) fractionated from sera of these ewes increased granulosa cell proliferation and steroidogenesis relative to the FA-free BSA control during culture, but there was no differential effect of n-3 and n-6 PUFAs on either oestradiol or progesterone production. HDL was ineffective in delivering FAs to embryos during culture, although n-6 PUFA HDL reduced embryo development. All blastocysts, irrespective of the treatment, contained high levels of unsaturated FAs, in particular linoleic acid. Transcripts for HDL and low-density lipoprotein (LDL) receptors (SCARB1 and LDLR) and stearoyl-CoA desaturase (SCD) are reported in sheep embryos. HDL reduced the expression of transcripts for LDLR and SCD relative to the BSA control. The data support a differential effect of n-3 and n-6 PUFAs on ovarian steroidogenesis and pre-implantation development, the latter in the absence of a net uptake of FAs.

show abstract

Section: Effects Of N-3 and N-6 Hdls During Embryo Culturementioning

confidence: 47%

Dietary omega-3 and -6 polyunsaturated fatty acids affect the composition and development of sheep granulosa cells, oocytes and embryos

Wonnacott¹,

Kwong²,

Hughes³

et al. 2010

Reproduction

129

104

View full text Add to dashboard Cite

show abstract

“…Additionally, palmitic acid and stearic acid were shown to have adverse effects on bovine granulosa cell in vitro growth and function , a phenomenon also found in human granulosa cells (Mu et al 2001) apoptosis. Moreover, previous studies report that competent human oocytes generally have lower levels of SFAs (Haggarty et al 2006). Comparison of the follicular fluid from the dominant and subordinate follicles showed that there was significantly less palmitic acid (40%) associated with the dominant follicle in cows.…”

Section: Discussionmentioning

confidence: 88%

Metabolite concentrations in follicular fluid may explain differences in fertility between heifers and lactating cows

Bender

Walsh²,

Evans³

et al. 2010

Reproduction

134

102

View full text Add to dashboard Cite

There has been a marked decline in the fertility of dairy cows over the past decades, and metabolomic analysis offers a potential to investigate the underlying causes. Metabolite composition of the follicular fluid, which presents the intrafollicular environment, may be an important factor affecting oocyte maturation and subsequent early embryo development. The aim of the present study was to investigate the metabolic differences between follicular fluid from the dominant follicle of lactating cows and heifers using gas chromatography mass spectrometry (GC-MS)-based metabolomics. Follicular fluid and serum were collected from cows and heifers over three phases of follicle development: newly selected dominant follicles, preovulatory follicles prior to oestrus and post-LH surge follicles. Analysis of the fatty acids revealed that there were 24 fatty acids and 9 aqueous metabolites significantly different between cows and heifers. Of particular interest were the higher concentrations of saturated fatty acids (palmitic acid, PZ0.001; stearic acid, PZ0.005) in follicular fluid from cows and higher docosahexaenoic acid levels (PZ0.022) in follicular fluid from heifers. Analysis of the metabolite composition of serum revealed that follicular fluid had a unique lipid composition. The higher concentrations of detrimental saturated fatty in cows will have a negative impact on oocyte maturation and early embryo development. Overall, the results suggest that the follicle microenvironment in cows potentially places their oocytes at a developmental disadvantage compared with heifers, and that this may contribute to well-characterised differences in fertility.

show abstract

“…It binds to various water‐insoluble substances like lipids. The lipids that are carried by albumin become an energy source and biosynthetic substances for an embryo 139, 140, 141. In addition, serum albumin serves as an antioxidant, osmotic regulator, and neutralizer of toxins.…”

Section: Current Issues With Culture Mediamentioning

confidence: 99%

Animal‐cell culture media: History, characteristics, and current issues

Yao

Asayama

2017

Reprod Medicine & Biology

346

278

View full text Add to dashboard Cite

BackgroundCell culture technology has spread prolifically within a century, a variety of culture media has been designed. This review goes through the history, characteristics and current issues of animal‐cell culture media.MethodsA literature search was performed on PubMed and Google Scholar between 1880 and May 2016 using appropriate keywords.ResultsAt the dawn of cell culture technology, the major components of media were naturally derived products such as serum. The field then gradually shifted to the use of chemical‐based synthetic media because naturally derived ingredients have their disadvantages such as large batch‐to‐batch variation. Today, industrially important cells can be cultured in synthetic media. Nevertheless, the combinations and concentrations of the components in these media remain to be optimized. In addition, serum‐containing media are still in general use in the field of basic research. In the fields of assisted reproductive technologies and regenerative medicine, some of the medium components are naturally derived in nearly all instances.ConclusionsFurther improvements of culture media are desirable, which will certainly contribute to a reduction in the experimental variation, enhance productivity among biopharmaceuticals, improve treatment outcomes of assisted reproductive technologies, and facilitate implementation and popularization of regenerative medicine.

show abstract

Fatty acid metabolism in human preimplantation embryos

Abstract: Preimplantation human embryos actively take up individual fatty acids at different rates at different stages of development. The high unsaturated concentration at the later stages of development may be explained by preferential uptake of linoleic acid.

Cited by 119 publications

References 24 publications

Dietary omega-3 and -6 polyunsaturated fatty acids affect the composition and development of sheep granulosa cells, oocytes and embryos

Dietary omega-3 and -6 polyunsaturated fatty acids affect the composition and development of sheep granulosa cells, oocytes and embryos

Metabolite concentrations in follicular fluid may explain differences in fertility between heifers and lactating cows

Animal‐cell culture media: History, characteristics, and current issues

Contact Info

Product

Resources

About