Fatty Acid-Free Bovine Albumin as Plasma Replacement for In vitro Cultivation of Plasmodium falciparum

Siddiqui, Wasim A.; Richmond-Crum, Suzanne M.

doi:10.2307/3280026

Cited by 11 publications

(7 citation statements)

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“…Because of these disadvantages, numerous alternative sources of sera and nonserum substitutes have been tested in the past (1,6,13,15,19,27,28,37). Although some of these substitutes have been successfully used for the continuous cultivation of laboratory-adapted P. falciparum strains and clones, they are generally less effective than human serum and have not been adopted by many research laboratories.…”

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In Vitro Culture and Drug Sensitivity Assay of Plasmodium falciparum with Nonserum Substitute and Acute-Phase Sera

et al. 1999

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The short-term in vitro growth of Plasmodium falciparumparasites in the asexual erythrocytic stage and the in vitro activities of eight standard antimalarial drugs were assessed and compared by using RPMI 1640 medium supplemented with 10% nonimmune human serum, 10% autologous or homologous acute-phase serum, or 0.5% Albumax I (lipid-enriched bovine serum albumin). In general, parasite growth was maximal with autologous (or homologous) serum, followed by Albumax I and nonimmune serum. The 50% inhibitory concentrations (IC50s) varied widely, depending on the serum or serum substitute. The comparison of IC50s between assays with autologous and nonimmune sera showed that monodesethylamodiaquine, halofantrine, pyrimethamine, and cycloguanil had similar IC50s. Although the IC50s of chloroquine, monodesethylamodiaquine, and dihydroartemisinin were similar with Albumax I and autologous sera, the IC50s of all test compounds obtained with Albumax I differed considerably from the corresponding values obtained with nonimmune serum. Our results suggest that Albumax I and autologous and homologous sera from symptomatic, malaria-infected patients may be useful alternative sources of serum for in vitro culture of P. falciparum isolates in the field. However, autologous sera and Albumax I do not seem to be suitable for the standardization of isotopic in vitro assays for all antimalarial drugs.

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confidence: 99%

In Vitro Culture and Drug Sensitivity Assay of Plasmodium falciparum with Nonserum Substitute and Acute-Phase Sera

et al. 1999

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“…On the basis of a different line of investigations-the analysis of involuntary changes in the posture of the human arm following changes in load-arm movements have been hypothesized to result from shifts in the equilibrium point of the muscle-load system (6). Finally, certain motor disorders that depend upon damage to the left hemisphere have been interpreted to result from pathological disruption of the process controlling sequential attainment of postures (7 We previously reported that the owl monkeys (Aotus trivirgatus griseimembra) could be immunized against a human malaria parasite (Plasmodium falciparum) infection (1), and this result was confirmed (2). In those studies Freund's complete adjuvant was essential for effective immunization.…”

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“…The immunizing antigen was prepared by short-term in vitro cultivation of this parasite in RPMI 1640 medium supplemented with fetal calf serum (FCS) and fatty acid-free bovine albumin (FAF albumin) (7). Parasitized blood from owl monkeys was cultured in sterile 500-ml side-arm flasks fitted with stoppers, with entry ports for a gas mixture of 90 percent N2, 8 percent CO2, and 2 percent 02.…”

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Vaccination of Experimental Monkeys Against Plasmodium falciparum : A Possible Safe Adjuvant

Siddiqui

Taylor

Kan

et al. 1978

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108

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Owl monkeys (Aotus trivirgatus griseimembra) were effectively immunized against a human malaria parasite, Plasmodium falciparum. Two injections of antigen, primarily mature segmenters with fully developed merozoites, mixed with adjuvant (6-O-stearoyl-N-acetylmuramyl-L-alanyl-D-isoglutamine and liposomes) were administered intramuscularly at a 4-week interval. Approximately 2 weeks after the second vaccination, the monkeys were challenged with the homologous strain of P. falciparum. All immunized monkeys survived the challenge. The substitution of Freund's complete adjuvant is an encouraging step toward the development of an effective and safe vaccine for human malaria.

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“…Because of the difficulties in obtaining type AB human sera from non-immune donors for malaria parasite cultivation, a number of studies have evaluated alternative sources of animal sera and non-serum substitutes. [18][19][20][21][22][23] Although some of these alternatives support parasite growth, their use has generally been confined to a few laboratories and have not replaced human serum for routine use. An important breakthrough was attained when Ofulla and others have identified serum albumin and lipids as the key serum components necessary for optimal parasite growth.…”

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confidence: 99%

Molecular Epidemiology of Malaria in Cameroon. Xv. Experimental Studies on Serum Substitutes and Supplements and Alternative Culture Media for in Vitro Drug Sensitivity Assays Using Fresh Isolates of Plasmodium Falciparum

Basco¹

2003

The American Journal of Tropical Medicine and Hygiene

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In vitro drug sensitivity assay is an important tool for various on-going studies aiming to establish the correlation between candidate molecular markers for drug resistance and drug response in laboratory-adapted strains and field isolates of Plasmodium falciparum. A widespread use of this technique in the field would require a suitable substitute that can replace human serum. In this study, several alternative sources of serum substitutes and supplements were evaluated for their capacity to sustain parasite growth for a single life cycle and their compatibility with in vitro assays for clinical isolates that have not been adapted to in vitro culture. Albumax, a commercial preparation of lipid-enriched bovine albumin, did not support parasite growth as much as human serum and fetal calf serum in several isolates. Other serum supplements (AmnioMax and Ultroser) supported parasite growth relatively well. The 50% inhibitory concentrations (IC50s) of chloroquine and antifolates determined with 0.05% Albumax were generally two or three times higher than with human serum. With 10% fetal calf serum, IC50s for chloroquine and antifolates were approximately two times higher and three times lower than with human serum, respectively. The use of AmnioMax and OptiMAb resulted in a greater than two-fold increase in IC50s and several uninterpretable assays. Despite possible batch-to-batch differences, fetal calf serum may be a suitable substitute for in vitro drug assays while awaiting the results of further studies on other serum substitutes and supplements.

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Fatty Acid-Free Bovine Albumin as Plasma Replacement for In vitro Cultivation of Plasmodium falciparum

Cited by 11 publications

References 0 publications

In Vitro Culture and Drug Sensitivity Assay of Plasmodium falciparum with Nonserum Substitute and Acute-Phase Sera

In Vitro Culture and Drug Sensitivity Assay of Plasmodium falciparum with Nonserum Substitute and Acute-Phase Sera

Vaccination of Experimental Monkeys Against Plasmodium falciparum : A Possible Safe Adjuvant

Molecular Epidemiology of Malaria in Cameroon. Xv. Experimental Studies on Serum Substitutes and Supplements and Alternative Culture Media for in Vitro Drug Sensitivity Assays Using Fresh Isolates of Plasmodium Falciparum

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