Fatty acid composition of serum cholesteryl ester fractions isolated by thin-layer chromatography

Alling, Christer; Svennerholm, Lars; Tichý, J

doi:10.1016/0021-9673(68)80080-9

Cited by 12 publications

(4 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Liver total cholesterol, free cholesterol and cholesteryl ester contents were determined by the method previously described [15,16]. Plasma cholesteryl ester was divided using a thin-layer chromatography technique [17]. The fatty acid composition of plasma cholesteryl ester was determined using gas chromatography.…”

Section: Discussionmentioning

confidence: 99%

Effect of Starvation-Refeeding Status on Cholesterol Metabolism in Rats Fed High-Cholesterol Diet

Inai¹,

Matsuo

2011

FNS

View full text Add to dashboard Cite

The present study investigated the effect of starvation-refeeding status on cholesterol metabolism in rats fed a high-cholesterol diet or a cholesterol-free diet. Twenty male and 20 female Donryu rats (age 5 weeks) were fed a cho-lesterol-free diet for 14 days. Then the male and female rats were each divided into two groups: feeding and starva-tion-refeeding groups. The feeding groups were fed the experimental diet for 3 days, and the starvation-refeeding groups fasted for 2 days followed by 3 days of feeding. Half of each of groups was fed a cholesterol-free diet and the other half was fed a high-cholesterol diet. Starvation-refeeding significantly increased the plasma free cholesterol and HDL-cholesterol concentrations in both the high-cholesterol-diet-fed rats and the cholesterol-free-diet-fed rats. In the female rats, plasma total cholesterol and cholesteryl ester concentrations were significantly higher in the high-cholesterol groups than in the cholesterol-free groups, whereas TG concentration and total cholesterol/TG ratio were not significantly different among all of the groups. Liver total cholesterol and cholesteryl ester were significantly higher in the high-cholesterol groups than in the cholesterol-free groups in both male and female rats. These results suggest that starvation-refeeding affected cholesterol metabolism at least in part. The reactivity of the cholesterol me-tabolism may be different between male and female rats

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of Starvation-Refeeding Status on Cholesterol Metabolism in Rats Fed High-Cholesterol Diet

Inai¹,

Matsuo

2011

FNS

View full text Add to dashboard Cite

show abstract

“…Liver total cholesterol, free cholesterol, and cholesteryl ester contents were determined by the method described previously [24,25]. Plasma cholesteryl ester was detemined using a thin-layer chromatography technique [26]. The fatty acid composition of plasma cholesteryl ester was determined using gas chromatography.…”

Section: Discussionmentioning

confidence: 99%

“…ACAT, an integrated membrane enzyme, is involved in regulating intracellular cholesterol homeostasis by catalyzing formation of cholesteryl ester from free cholesterol and long-chain acyl-CoA. A high-cholesterol diet is associated with increased hepatic microsomal ACAT activity [28]. DeWitt et al [29] suggested that stimulation of ACAT activity was only observed with the addition of oleic acid, but not with palmitic, stearic, or linoleic acids in vitro.…”

Section: Discussionmentioning

confidence: 99%

Effects of High-Fat Diets Containing Different Fats on Cholesterol Metabolism in Starvation-Refeeding Rats

Inai¹,

Matsuo

2011

FNS

View full text Add to dashboard Cite

The present study was performed to investegate the effects of high-fat diets containing different fats on cholesterol metabolism in starvation-refeeding rats. Forty female Donryu rats were divided into two groups and then fed high-fat diets containing beef tallow or corn oil without cholesterol for 14 days. Then, 10 rats from each group were divided into high-cholesterol and cholesterol-free groups (Experiment 1). Another 10 rats from beef tallow and corn oil groups were divided into high-cholesterol and high-cholesterol-cholestyramine groups (Experiment 2). All rats were fasted for 2 days followed by 3 days of feeding. In Experiment 1, the high-cholesterol diet caused significant increases in plasma total cholesterol and cholesteryl ester concentrations in the beef tallow diet group. In Experiment 2, dietary cholestyramine markedly decreased plasma and liver cholesterol levels; however, these cholesterol levels were higher in the beef tallow diet group even if cholestyramine was added to the diet. These results suggested that the cholesterol- lowering effect of dietary corn oil may not be due solely to reabsorption of bile acids. This study suggested that high-fat diets containing different fats affected cholesterol metabolism under conditions of starvation-refeeding

show abstract

“…The amount of neutral lipids was determined by oxidation with acid dichromate reagent and that of the phospholipids by measuring their p content. Cholesterol esters were separated with n-heptane:toluene 60:25 mixture (Alling et a/., 1968) and were visualized by 10% ethanolic phosphot ungst ic acid.…”

Section: Lipid Extraction and Fractionationmentioning

confidence: 99%

Lipid analysis of cell nuclei in young and old Ehrlich ascites tumors

Z¹,

Holczinger²

1974

Intl Journal of Cancer

View full text Add to dashboard Cite

Fatty acid composition of serum cholesteryl ester fractions isolated by thin-layer chromatography

Cited by 12 publications

References 5 publications

Effect of Starvation-Refeeding Status on Cholesterol Metabolism in Rats Fed High-Cholesterol Diet

Effect of Starvation-Refeeding Status on Cholesterol Metabolism in Rats Fed High-Cholesterol Diet

Effects of High-Fat Diets Containing Different Fats on Cholesterol Metabolism in Starvation-Refeeding Rats

Lipid analysis of cell nuclei in young and old Ehrlich ascites tumors

Contact Info

Product

Resources

About