Fatty acid composition of meat and genetic mapping of quantitative trait loci in 3 generations of Japanese quail populations

Abstract

The present study was carried out to evaluate the effect of different lines and generations of adult males and females of Japanese quail (Coturnix japonica) on total lipid, fatty acid (FA) composition and cholesterol content of breast muscle, as well as the identification of quantitative trait loci (QTLs) controlling the above mentioned meat quality traits. Forty-four quails (generation F 0 ), 22 Pharaoh (F-33) meat-type males and 22 Standard (S-22) laying-type females, were crossed to produce the F 1 hybrids generation. F 2 generation was created by mating one F 1 male with one F 1 female, full siblings. The birds, randomly chosen from F 0 (22 males and 22 females), F 1 (22 males and 22 females) and F 2 (84 males and 152 females), were raised to 20 weeks of age in collective cages. After slaughter the carcasses were dissected and the breast muscle was removed for the chemical analysis. Genomic DNA was extracted from the whole blood and 30 microsatellite markers located on two quail chromosomes were genotyped. S-22 quails exhibited higher amounts of total saturated fatty acids (SFA) and polyunsaturated fatty acids (PUFA), while F-33 quails had a greater content of total monounsaturated fatty acids (MUFA). F-33 quails supplied meat with lower atherogenic and thrombogenic indexes. A partial effect of sex on the FA composition of quail meat was found in the F 1 generation; while in the F 2 generation a sex-effect was more evident. F 2 females exhibited a higher PUFA content and PUFA/SFA ratio, and a lower muscle cholesterol content compared to F 2 males. In conclusion, total lipid, FA composition and cholesterol content were affected by first-and second-generation crosses. For the cholesterol content a QTL showing additive effect has been detected on CJA02 at 85 cM; no QTLs were found for total SFA, MUFA and PUFA. To current knowledge, this is the first study of a QTL associated with muscle cholesterol in quails.

The composition of the lipid, protein and mineral fractions of quail breast meat obtained from wild and farmed specimens of Common quail (Coturnix coturnix) and farmed Japanese quail (Coturnix japonica domestica)

“…The h/H ratio and the AI were not significantly influenced ( P > 0.05) by neither the quail species nor by the origin within CQ. The results observed herein on farmed quail fatty acid ratios and lipid quality indices are within the range of values previously presented by Tavaniello et al (2017) .…”

“…On the other hand, significant difference ( P = 0.031) on breast meat total cholesterol was observed between wild and farmed specimens within CQ, farmed specimens presented a superiority of 12.9% on the total cholesterol content (more 8.32 mg/g of meat). The total cholesterol content observed in quail's breast meat obtained from farmed quails (71.1–72.7 mg/100 g of meat) is inside the range of cholesterol content previously presented in quail's breast meat (67.2–76 mg/100 g of meat) for domestic quails ( Coturnix japonica ) ( Ioniţă et al, 2010 ; Fakolade, 2015 ; Tavaniello et al, 2017 ; Cullere et al, 2018 ). Whereas, the total cholesterol content observed in wild CQ (64.4 mg/100 g of meat) is quite below that range, but we found no comparison for it.…”

Carcass characteristics and meat quality assessment in different quail lines fed on canola seed supplemented diets

“…Regardless of the treatment, the effect of the line variation on nutritional composition was insignificant. A similar trend was also reported by Tavaniello et al (2017) who found no effect of different lines of Japanese quail on breast muscle total lipid content and fatty acid profiles. Repeated measures analysis showed no significant (P>0.05) diet × line interaction effect on nutritional composition of breast and thigh meat proximate composition and omega-3 and omega-6 (Table 4 and 5).…”

Effect of faba bean-based diets on the meat quality and fatty acids composition in breast muscles of broiler chickens

“…Moreover, the atherogenic index (AI) and the thrombogenic index (TI) were calculated 26 . Method used for fatty acid composition were done in the same way as it was described by Tavaniello et al 33 .…”

“…The quantitation of cholesterol content in muscles was based on the external standard method using a pure cholesterol standard (Sigma, St. Louis, MO). This method was also done according to the method of Tavaniello et al 33 .…”