Fatty acid composition of lipids in tongue and hindleg muscles of muscular dystrophic mice

Futo, Tomomichi; Hitaka, Teruaki; Mizutani, Takaharu; Okuyama, Harumi; Watanabe, Kimi; Totsuka, Tsuyoshi

doi:10.1016/0022-510x(89)90062-2

Cited by 7 publications

(5 citation statements)

References 16 publications

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“…It has been reported that the concentrations of lipid peroxides and malondialdehyde are high in dystrophic muscle [10,[28][29][30]. These results are in agreement with our previous findings of a low docosahexaenoic acid content in phospholipids [13]. Thus membranes in dystrophic muscles are regarded as being exposed to such a toxic oxidative environment.…”

Section: Discussionsupporting

confidence: 91%

“…Our previous paper demonstrated that the content of docosahexaenoic acid in phospholipid was low in skeletal muscle ofdystrophic mice whereas there was no difference in its content between tongue muscles of dystrophic and normal mice [13]. This result supports the bone-muscle imbalance hypothesis proposed previously by Totsuka [26].…”

Section: Discussionsupporting

confidence: 87%

“…Polyunsaturated fatty acids in the phospholipids of membranes are especially susceptible to such damage by peroxidation. We have previously reported a decrease in the docosahexaenoic acid content in phospholipids of skeletal muscles in dystrophic mice [13]. It has also been reported that the content of GSHPx relevant to the decomposition of hydroperoxide is increased in human dystrophic muscles [11,14].…”

Section: Introductionmentioning

confidence: 86%

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The high content of natural suppressor serine tRNA in dystrophic mouse muscle

Hitaka

Mizutani

Watanabe

et al. 1990

Biochemical Journal

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In order to gain an insight into the pathogenesis of mouse muscular dystrophy, we investigated the natural suppressor serine tRNA. The natural suppressor seryl-tRNA was distinguished from the other seryl-tRNAs on the basis of its specific property of being converted into phosphoseryl-tRNA by a tRNA kinase. On a wet-weight basis, the content of total tRNA in dystrophic muscles was 47% of that in normal muscles. Although the serine-accepting activities of tRNA were similar in muscles of 3-month-old dystrophic and normal mice, the ratio of [32P]phosphoseryl-tRNA (suppressor tRNA) to the total serine tRNA was significantly enhanced in dystrophic muscles compared with that in normal muscles. This high content of suppressor tRNA in dystrophic muscles was further confirmed by dot-blot hybridization experiments with the DNA probes CGTAGTCGGCAGGAT and CGCCCGAAAGGTGGAA for major tRNA(IGASer) and suppressor tRNA respectively. At the early postnatal age of 3 weeks, when only a week had elapsed since the first manifestation of the dystrophic symptom (hindleg dragging), the ratio of suppressor tRNA to major tRNAs in dystrophic hindleg muscles was abnormally increased. Thereafter it decreased with age in normal mice but remained almost unchanged in dystrophic mice. Consequently, at 3 months old, it was 1.7 times higher in dystrophic than in normal mice. The suppressor tRNA is now accepted to play a role in the synthesis of glutathione peroxidase. The present study showed that the content of this enzyme was abnormally elevated in dystrophic mice. Previously we had demonstrated that the docosahexaenoic (C22:6) acid content in phospholipids was decreased, possibly resulting from the enhanced oxidative milieu caused by the dystrophic condition. Thus far, the findings suggest that an increase in the contents of suppressor tRNA and glutathione peroxidase in dystrophic muscle may have been secondarily induced by such a highly oxidative state in the dystrophic condition. However, it is difficult to exclude the possibility that the natural suppressor tRNA plays a primary role in the pathogenesis of muscular dystrophies.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 86%

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The high content of natural suppressor serine tRNA in dystrophic mouse muscle

Hitaka

Mizutani

Watanabe

et al. 1990

Biochemical Journal

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“…large-scale replacement of contractile tissue with fat and connective tissue [28,29]. These effects may be associated with changes of local fiber organization, resulting in reduced diffusion anisotropy, i.e., increased randomness of fiber direction.…”

Section: Discussionmentioning

confidence: 99%

Three-Dimensional Muscular Architecture of the Human Tongue Determined In Vivo With Diffusion Tensor Magnetic Resonance Imaging

Gilbert

Napadow

2005

Dysphagia

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The myoarchitecture of the tongue is believed to consist of a complex network of interwoven fibers, which function together to produce a near limitless array of functional deformations. These deformations contribute mechanically to speech production and to oral cavity food handling during swallowing. We have previously imaged the 3D myoarchitecture of the mammalian tongue in excised tissue with diffusion tensor MRI, a technique which derives the 3D orientation of intramural fibers as a function of the extent to which a direction-specific MR signal attenuates under diffusion-encoding magnetic gradients. The resulting 3D diffusion tensor defines the relative orientations of the myofiber populations within a region of tissue. In this study, we have extended the use of this method to assess lingual myoarchitecture in normal human subjects in vivo. Subjects were imaged using a diffusion-sensitive stimulated-echo pulse sequence with single-shot echo-planar spatial encoding in the midsagittal plane. Differences in lingual fiber orientation were manifested by graduated changes in fiber direction throughout the tissue, without clear anatomical demarcations between regions of the tissue. The anterior tissue was composed generally of orthogonally oriented fibers surrounded by an axially oriented ring of tissue, whereas the posterior portion of the tissue was composed mostly of fibers projecting in the superior and posterior directions. The bulk of the tissue displayed a highly homogeneous, vertically oriented set of fibers, including the anteroinferior region of the tissue and extending nearly to the superior surface. Further analysis of the tissue in terms of diffusion anisotropy demonstrated that the tissue could be represented by varying degrees of anisotropy, with a tendency toward high anisotropy in the dorsal and anteroventral periphery and low anisotropy in the central region of the tissue. These findings demonstrate that the muscular anatomy of the tongue can be displayed as a continuous array of structural units, or tensors, representing fibers of varying orientations throughout the tissue.

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“…4) Meanwhile, docosahexaenoic acid (22 : 6, DHA), n- Vol. 49 (2003) 3 fatty acid, is rich in the retina, brain and muscle, [5][6][7] and is important to maintain the good condition of these organs. 8) Eicosapentaenoic acid (20 : 5, EPA), another n-3 fatty acid, also plays a key role in preventing thrombosis in veins.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Dietary Fatty Acids on the Expression Levels and Activities of Hepatic Drug Metabolizing Enzymes.

Katô

Ikemoto

Mizutani

2003

JOURNAL OF HEALTH SCIENCE

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Analysis of the fatty acid composition in the microsomes of rats fed the oil rich in α-linolenic acid (n-3, perilla oil, Per) or linoleic acid (n-6, safflower oil, Saf) showed changes according to those compositions in the diets. The level of phase I enzyme in microsomes prepared from rat liver was measured by the Western blotting method. The levels of CYP1A1, 1A2 and 2E1 were similar between the two oil groups, Per and Saf. Meanwhile, the level of CYP4A1 in the microsomes of rats fed the Per diet was significantly higher than that from rats fed Saf. As a second step, we measured the level and activity of UDP-glucuronosyltransferase (UGT). The results showed that the level of UGT activity in microsomes from rats fed the Per diet was 2.4-fold higher than that from rats fed the Saf diet. The protein level of UGT in microsomes from rats fed the Per diet was also 1.7-fold greater than that from rats fed the Saf diet. The low level of protein amount and activity of UGT in rats fed Saf was recovered by the administration of the Per diet. Furthermore, the UGT activity in microsomes from rats fed high docosahexaenoic acid, another n-3 fatty acid, was higher than that in rats fed Per oil. These results suggest that the fatty acid composition, n-3 or n-6, in foods influences the level and activity of CYP4A and UGT. Thus, monitoring of the drug level in the blood of patients in consideration of oil intake should be undertaken.

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Fatty acid composition of lipids in tongue and hindleg muscles of muscular dystrophic mice

Cited by 7 publications

References 16 publications

The high content of natural suppressor serine tRNA in dystrophic mouse muscle

The high content of natural suppressor serine tRNA in dystrophic mouse muscle

Three-Dimensional Muscular Architecture of the Human Tongue Determined In Vivo With Diffusion Tensor Magnetic Resonance Imaging

The Effect of Dietary Fatty Acids on the Expression Levels and Activities of Hepatic Drug Metabolizing Enzymes.

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