Fatty acid composition of developing tree peony (Paeonia section Moutan DC.) seeds and transcriptome analysis during seed development

Abstract

BackgroundTree peony (Paeonia section Moutan DC.) is known for its excellent ornamental and medicinal values. In 2011, seeds from P. ostii have been identified as novel resource of α-linolenic acid (ALA) for seed oil production and development in China. However, the molecular mechanism on biosynthesis of unsaturated fatty acids in tree peony seeds remains unknown. Therefore, transcriptome data is needed to better understand the underlying mechanisms.ResultsIn this study, lipid accumulation contents were measured using GC-MS methods across developing tree peony seeds, which exhibited an extraordinary ALA content (49.3%) in P. ostii mature seeds. Transcriptome analysis was performed using Illumina sequencing platform. A total of 144 million 100-bp paired-end reads were generated from six libraries, which identified 175,874 contigs. In the KEGG Orthology enrichment of differentially expressed genes, lipid metabolism pathways were highly represented categories. Using this data we identified 388 unigenes that may be involved in de novo fatty acid and triacylglycerol biosynthesis. In particular, three unigenes (SAD, FAD2 and FAD8) encoding fatty acid desaturase with high expression levels in the fast oil accumulation stage compared with the initial stage of seed development were identified.ConclusionsThis study provides the first comprehensive genomic resources characterizing tree peony seeds gene expression at the transcriptional level. These data lay the foundation for further understanding of molecular mechanism responsible for lipid biosynthesis and the high unsaturated fatty acids (especially ALA) accumulation. Meanwhile, it provides theoretical base for potential oilseed application in the respect of n-6 to n-3 ratio for human diets and future regulation of target healthy components of oils.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1429-0) contains supplementary material, which is available to authorized users.

Comparative transcriptome and coexpression network analysis of carpel quantitative variation in Paeonia rockii

“…These DEGs were reference candidate genes that synergistically regulated the carpel quantitative variation in P. rockii. Because there have been no genomic information of Paeonia yet, RNA-seq technology has become an important means of screening candidate genes associated with key traits [40,42]. However, huge genomic information (about 12.5 G) and complex genetic background make it very difficult to identify gene loci effectively by the NGS method only.…”

“…It also identified a large number of novel long non-coding RNAs and fusion transcripts [39]. At present, only NGS and SLAF-seq technologies have been applied frequently in tree peonies [40][41][42][43]. It is the first attempt to explore the carpel quantitative variation by SMLRS.…”

Consumer properties improvement of sugar cookies with fillings with non-traditional raw materials with high biological value

“…These include Sacha Inchi oil, peony oil, sea buckthorn oil, cypress oil, cress salad oil. The content of α-linolenic acid is 50; 45; 35; 30 % in these oils [17]. The sea buckthorn oil effect on the fatty acid content of lipids is little known.…”

Tolerance strategies revealed in tree peony (Paeonia suffruticosa; Paeoniaceae) ecotypes differentially adapted to desiccation

“…Transcriptome analysis that uses deep sequencing technology now permits large‐scale gene expression detection in the absence of a reference genome. Although there have been several investigations of transcriptome sequencing of tree peony (Gai et al., ; Zhou et al., ; Zhang et al., , ; Zhao et al., ; Barghini et al., ; Li et al., ; Shi et al., ; Gao et al., ; Wang et al., ), studies of drought‐responsive differential expression genes in tree peonies have not yet been reported in the literature. Two separate studies of reference gene selection in tree peony—one in plants with different flower colors and another during flower development—were recently reported (Li et al., ; Zhou et al., ).…”